IMMUNOHEMATOLOGY MIDTERM EXAM
QUESTIONS AND ANSWERS
Hemagglutination is a two step process involving sensitization and agglutination.
Choose the answer below that influences stage 2 agglutination.
a) Temperature
b) Ionic strength
c) Time
d) pH
e) Immunoglobulin Class - Correct Answers -Immunoglobulin class
Which describes the function of PEG, a potentiator used in immunohematologic testing?
a) Generates vasoactive amines
b) Reduces net negative charge on RBCs
c) Reduces pH of test environment
d) Decreases water molecules surrounding the RBCs
e) Increases the dielectric constant of the medium - Correct Answers -Decreases water
molecules surrounding the RBCs
These molecules are usually not antigenic substances:
a) Glycoproteins
b) Protein
c) Lipids
d) Carbohydrates - Correct Answers -Lipids
Anti-A: 4+ | Anti-B: - | A1 cells: - | B cells: 4+ | Screen cells: 2+ | Autocontrol: -
These results are most likely due to:
a) Alloantibody
b) Autoantibody
c) Idioantibody
d) Rouleax - Correct Answers -Alloantibody
Individuals who are Le(a-b-) can develop anti-Lewis without exposure to Lewis antigens
are called
a) Immune mediated
b) Biphasic
c) Naturally occurring
, d) Autoantibodies - Correct Answers -Naturally occurring
If a patient has an antibody in the Kidd system, which panel cells with serum will detect
this antibody only after which 2 stages of the IAT?
a) Incubation at 4C and addition of anti-Jk
b) Incubation at 25C and the addition of A cells
c) Incubation at 37C and the addition of AHG
d) Incubation at 37C and the addition of check cells - Correct Answers -Incubation at
37C and the addition of AHG
The IAT is used to detect ______ sensitization of RBCs - Correct Answers -In vitro
The statement: "Contains anti-IgG and anti-C3d" best describes which of the following
antiglobulin reagents?
a) Triclonal antihuman globulin
b) Polyspecific antihuman globulin
c) Monoclonal antihuman globulin
d) Murine hybridoma trisomal - Correct Answers -Polyspecific antihuman globulin
Which of the following is *not true* of the H antigen?
a) the amount of H antigen varies for different ABO types
b) inheritance of H is necessary for the normal expression of ABO antigens
c) anti-H antibody is naturally occurring in the serum of most adults
d) H antigen may be present in secretions - Correct Answers -anti-H antibody is
naturally occurring in the serum of most adults
The immunodominant sugar that confers A blood group specificity is: - Correct Answers
-N-acetylgalactosamine
The ____ antigen is found in 85% of the white population, 92% in the black population,
is always matched for transfusion, has strong expression at birth & is not destroyed by
enzymes. It is also very immunogenic.
a) K
b) Fya
c) k
d) C
e) D - Correct Answers -D
If a patient has multiple antibodies, percentage of compatible units must be determined.
Percentage of compatible units is ____ or ____% of units that are compatible with a
patient that requires E-, M-, c- units.
QUESTIONS AND ANSWERS
Hemagglutination is a two step process involving sensitization and agglutination.
Choose the answer below that influences stage 2 agglutination.
a) Temperature
b) Ionic strength
c) Time
d) pH
e) Immunoglobulin Class - Correct Answers -Immunoglobulin class
Which describes the function of PEG, a potentiator used in immunohematologic testing?
a) Generates vasoactive amines
b) Reduces net negative charge on RBCs
c) Reduces pH of test environment
d) Decreases water molecules surrounding the RBCs
e) Increases the dielectric constant of the medium - Correct Answers -Decreases water
molecules surrounding the RBCs
These molecules are usually not antigenic substances:
a) Glycoproteins
b) Protein
c) Lipids
d) Carbohydrates - Correct Answers -Lipids
Anti-A: 4+ | Anti-B: - | A1 cells: - | B cells: 4+ | Screen cells: 2+ | Autocontrol: -
These results are most likely due to:
a) Alloantibody
b) Autoantibody
c) Idioantibody
d) Rouleax - Correct Answers -Alloantibody
Individuals who are Le(a-b-) can develop anti-Lewis without exposure to Lewis antigens
are called
a) Immune mediated
b) Biphasic
c) Naturally occurring
, d) Autoantibodies - Correct Answers -Naturally occurring
If a patient has an antibody in the Kidd system, which panel cells with serum will detect
this antibody only after which 2 stages of the IAT?
a) Incubation at 4C and addition of anti-Jk
b) Incubation at 25C and the addition of A cells
c) Incubation at 37C and the addition of AHG
d) Incubation at 37C and the addition of check cells - Correct Answers -Incubation at
37C and the addition of AHG
The IAT is used to detect ______ sensitization of RBCs - Correct Answers -In vitro
The statement: "Contains anti-IgG and anti-C3d" best describes which of the following
antiglobulin reagents?
a) Triclonal antihuman globulin
b) Polyspecific antihuman globulin
c) Monoclonal antihuman globulin
d) Murine hybridoma trisomal - Correct Answers -Polyspecific antihuman globulin
Which of the following is *not true* of the H antigen?
a) the amount of H antigen varies for different ABO types
b) inheritance of H is necessary for the normal expression of ABO antigens
c) anti-H antibody is naturally occurring in the serum of most adults
d) H antigen may be present in secretions - Correct Answers -anti-H antibody is
naturally occurring in the serum of most adults
The immunodominant sugar that confers A blood group specificity is: - Correct Answers
-N-acetylgalactosamine
The ____ antigen is found in 85% of the white population, 92% in the black population,
is always matched for transfusion, has strong expression at birth & is not destroyed by
enzymes. It is also very immunogenic.
a) K
b) Fya
c) k
d) C
e) D - Correct Answers -D
If a patient has multiple antibodies, percentage of compatible units must be determined.
Percentage of compatible units is ____ or ____% of units that are compatible with a
patient that requires E-, M-, c- units.
