IMMUNOHEMATOLOGY LAB PRACTICAL 1
REVIEW EXAM
what is a 3% red cell suspension? - Correct Answers -an approximation achieves the
appropriate serum-to-cell ratio for the most test procedures an adequate number of red
cells so one can read and grade reactions.
- agglutination of antigen-antibody complex requires optimal proportions.
What is the purpose of adding LISS to IAT? - Correct Answers -use of a LISS additive
accelerates antibody binding to red cells
What are acceptable positive reactions for forward typing? - Correct Answers -3-4+
what are acceptable positive reactions for reverse typing? - Correct Answers -2-4+
A discrepancy may be present if: - Correct Answers -- Agglutination is weaker than
expected
- Expected reactions are missing
- Extra reactions are noted
reasons for different current ABO group from historic ABO group - Correct Answers --
patient/sample identification error during collection
- patient is victim or perpetrator of identity theft
- sample mix-up during testing
- patient has received an ABO nonidentical bone marrow transplant
What phenotypes will agglutinate when combined with the lectin Dolichos biflorus? -
Correct Answers -A1 and A1B
What is the only antigen of the Rh system that is included in routine blood bank tests? -
Correct Answers -D
forward grouping is defined as - Correct Answers -using known sources of reagent
antisera/antibodies to detect antigens on an individual's red cells
reverse grouping is defined as - Correct Answers -using reagent red cells with known
ABO antigens and testing the serum of a patient for ABO antibodies
reactions of 2+ or less may indicate - Correct Answers -the reaction is due to an
antibody other than anti-A or anti-B
REVIEW EXAM
what is a 3% red cell suspension? - Correct Answers -an approximation achieves the
appropriate serum-to-cell ratio for the most test procedures an adequate number of red
cells so one can read and grade reactions.
- agglutination of antigen-antibody complex requires optimal proportions.
What is the purpose of adding LISS to IAT? - Correct Answers -use of a LISS additive
accelerates antibody binding to red cells
What are acceptable positive reactions for forward typing? - Correct Answers -3-4+
what are acceptable positive reactions for reverse typing? - Correct Answers -2-4+
A discrepancy may be present if: - Correct Answers -- Agglutination is weaker than
expected
- Expected reactions are missing
- Extra reactions are noted
reasons for different current ABO group from historic ABO group - Correct Answers --
patient/sample identification error during collection
- patient is victim or perpetrator of identity theft
- sample mix-up during testing
- patient has received an ABO nonidentical bone marrow transplant
What phenotypes will agglutinate when combined with the lectin Dolichos biflorus? -
Correct Answers -A1 and A1B
What is the only antigen of the Rh system that is included in routine blood bank tests? -
Correct Answers -D
forward grouping is defined as - Correct Answers -using known sources of reagent
antisera/antibodies to detect antigens on an individual's red cells
reverse grouping is defined as - Correct Answers -using reagent red cells with known
ABO antigens and testing the serum of a patient for ABO antibodies
reactions of 2+ or less may indicate - Correct Answers -the reaction is due to an
antibody other than anti-A or anti-B
