Bio 189
Questions
Exam 4and
–Study
Practice
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Bio 189 Exam 4 – Practice Questions and Study Guide
Bio 189 Exam 4 – Practice Questions and Study Guide
Bio 189 Exam 4 – Practice Questions and Study Guide
What i s a gene? Where i s i t found i n? A l i near sequence of DNA nucl eoti des; found i n chromati n/ strands of DNA
What happens when a cel l expresses a gene? I t makes protei ns
A gene sequence changed, what i s expected to happen to the protei n product Wi l l not functi on properl y
Overal l f l ow of i nf ormati on duri ng gene expressi on/ protei n synthesi s? DNA➡RNA➡Ami no Aci ds
1. mRN A: messenger, uses DNA as a templ ate to pol ymeri ze RNA; transcri pti on
2. rRNA: ri bosome, acts as enz yme compl ex to pol ymeri ze ami no aci ds i nto
3 forms of RNA; rol es; whi ch are i nvol ved wi th transcri pti on/ transl ati on? pol ypepti des; transl ati on
3. tRNA: transf er, transf ers ami no aci ds f rom cytopl asm to ri bosomes for
pol ymeri zati on; transl ati on
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,Bio 189 Exam 4 – Practice
Bio 189
Questions
Exam 4and
–Study
Practice
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What i s a codon? What RNA i s codon found i n? 3-l ettered word sequence used to make ami no aci ds; found i n mRN A
What i s an anti codon? Whi ch RNA i s i t found i n? 3 l ettered word sequence compl ementary to codon, found i n tRNA
Transcri pti on & where does i t occur i n? Makes mRN A i n nucl eus
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, Bio 189 Exam 4 – Practice
Bio 189
Questions
Exam 4and
–Study
Practice
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1. I ni ti ati on: RNA pol ymerase & transcri pti on factors attach to promoter (@TATA)
to unz i p DNA hel i x
3 sequenti al processes of transcri pti on & what happens i n each step? 2. El ongati on: RNA pol ymerase reads DNA templ ate 3'➡5' and pol ymeri zes RNA
5'➡3'. F orms 1° transcri pt
3. Termi nati on: RNA pol ymerase detaches, rel easi ng 1° RNA transcri pt
Promoter Si te on DNA that RNA pol ymerase can bi nd to, to i ni ti ate transcri pti on
What must happen to mRN A after i t i s transcri bed Must be processed further
Transcri be thi s sequence i nto mRN A
AUG-UAU-UUU-CCG-GGC-UAG
TAC-ATA-AAA-GGC-CCG-ATC
SPLI CESOMES HAPPEN
1. I ntrons are exci sed out
What happens duri ng RNA processi ng? STEPS
2. Exons are spl i ced together
3. Cap & tai l i s added to ends
Where does RNA processi ng occur? Nucl eus
What cel l ul ar components carry out RNA processi ng? Spl i cesomes
What are Exons? Are the exci sed or spl i ced? DNA nucl eoti de sequences used to make protei ns; spl i ced together
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