AAB MT ACTUAL PAPER Questions with Correct Answers (Grade
A+)
Question 1: What disorder does a defect in heme synthesis lead to?
Answer: Porphyria
Question 2: Porphyria
Answer: Deficiency in the enzymes of the porphyrin pathway leads to insufficient production of heme. The
principal problem in these deficiencies is the accumulation of porphyrins, the heme precursors, which are
toxic to tissue in high concentrations.
Question 3: What is methemoglobin?
Answer: Methemoglobin is a form of hemoglobin that contains ferric [Fe3+] iron and has a decreased
ability to bind oxygen. However, the ferric iron has an increased affinity for bound oxygen. When
methemoglobin concentration is elevated in red blood cells, tissue hypoxia can occur.
Question 4: What is the function of the enzyme methemoglobin-cytochrome C reductase in a red blood
cell?
Answer: To keep iron in the ferrous state
Question 5: What product is formed when ferrous iron of hemoglobin is oxidized to the ferric state?
Answer: Methemoglobin
Question 6: What is carboxyhemoglobin?
Answer: A stable complex of carbon monoxide and hemoglobin that forms in RBCs when carbon monoxide
is inhaled or produced in normal metabolism. Large quantities of it hinder delivery of O2 to the body.
Tobacco smoking (through carbon monoxide inhalation) raises the blood levels of COHb by a factor of
several times from its normal concentrations.
Question 7: Which hemoglobin has a 200 times greater binding capacity for the hemoglobin molecule
than oxygen?
Answer: Carboxyhemoglobin
Question 8: Which hemoglobin derivative causes irreversible changes in RBCs?
Answer: Sulfhemoglobin -- makes blood incapable of carrying oxygen.
Page 1
,Question 9: Which of the following hemoglobins is NOT normally present in a normal adult?
Answer: Hb-S
Question 10: Which embryonic hemoglobin is normally found in newborns?
Answer: Hb-F
Question 11: What is the most abundant hemoglobin in a newborn?
Answer: Hb-F
Question 12: What is the molecular structure of Hemoglobin S?
Answer: Valine replaces glutamic acid in the 6th position of the beta chain
Question 13: Which of the following hemoglobins is insoluble under lowered oxygen tension?
Answer: Hb-S
Question 14: What laboratory test is used to confirm the specific diagnosis of sickle cell anemia?
Answer: Hemoglobin electrophoresis
Question 15: In the solubility test for hemoglobin S, what is the reagent responsible for the reduction of
the hemoglobin molecule?
Answer: Sodium dithionite
Question 16: In hemoglobin C, what replaces glutamic acid?
Answer: Lysine
Question 17: Which red blood cell inclusion is characteristic of hemoglobin C disease?
Answer: Rod-shaped crystals
Question 18: What is hemoglobin F composed of?
Answer: 2 alpha and 2 gamma chains
Question 19: Which hemoglobin is resistant to alkali denaturation?
Answer: Hb-F
Page 2
, Question 20: Which hemoglobins are differentiated by the Kleihauer-Betke technique?
Answer: Hb-A1 from Hb-F
Question 21: What is the order of migration of hemoglobins, from fastest to slowest, on cellulose acetate
at pH 8.4?
Answer: A1, F, S, C
Question 22: Why is citrate agar used when abnormal hemoglobins are identified on cellulose acetate at
pH 8.6?
Answer: It separates hemoglobin S from hemoglobin D
Question 23: The cyanmethemoglobin method measures all hemoglobin pigments EXCEPT which one?
Answer: Sulfhemoglobin
Question 24: What is the diluent used in the cyanmethemoglobin method for the determination of
hemoglobin?
Answer: Drabkin's solution
Question 25: What is the normal plasma hemoglobin concentration?
Answer: 2-3 mg/dL
Question 26: Which plasma protein binds free hemoglobin?
Answer: Haptoglobin
Question 27: Which one of the following statements concerning hemolytic anemia is false?
Answer: The serum haptoglobin is increased
Question 28: In this type of anemia RBCs are normocytic and normochromic. RBCs, hemoglobin, and
hematocrit are decreased and the indirect bilirubin is increased.
Answer: Hemolytic anemia
Question 29: Myoglobin can be separated from hemoglobin by saturating the specimen with which one
of the following reagents?
Answer: Ammonium sulfite
Page 3
A+)
Question 1: What disorder does a defect in heme synthesis lead to?
Answer: Porphyria
Question 2: Porphyria
Answer: Deficiency in the enzymes of the porphyrin pathway leads to insufficient production of heme. The
principal problem in these deficiencies is the accumulation of porphyrins, the heme precursors, which are
toxic to tissue in high concentrations.
Question 3: What is methemoglobin?
Answer: Methemoglobin is a form of hemoglobin that contains ferric [Fe3+] iron and has a decreased
ability to bind oxygen. However, the ferric iron has an increased affinity for bound oxygen. When
methemoglobin concentration is elevated in red blood cells, tissue hypoxia can occur.
Question 4: What is the function of the enzyme methemoglobin-cytochrome C reductase in a red blood
cell?
Answer: To keep iron in the ferrous state
Question 5: What product is formed when ferrous iron of hemoglobin is oxidized to the ferric state?
Answer: Methemoglobin
Question 6: What is carboxyhemoglobin?
Answer: A stable complex of carbon monoxide and hemoglobin that forms in RBCs when carbon monoxide
is inhaled or produced in normal metabolism. Large quantities of it hinder delivery of O2 to the body.
Tobacco smoking (through carbon monoxide inhalation) raises the blood levels of COHb by a factor of
several times from its normal concentrations.
Question 7: Which hemoglobin has a 200 times greater binding capacity for the hemoglobin molecule
than oxygen?
Answer: Carboxyhemoglobin
Question 8: Which hemoglobin derivative causes irreversible changes in RBCs?
Answer: Sulfhemoglobin -- makes blood incapable of carrying oxygen.
Page 1
,Question 9: Which of the following hemoglobins is NOT normally present in a normal adult?
Answer: Hb-S
Question 10: Which embryonic hemoglobin is normally found in newborns?
Answer: Hb-F
Question 11: What is the most abundant hemoglobin in a newborn?
Answer: Hb-F
Question 12: What is the molecular structure of Hemoglobin S?
Answer: Valine replaces glutamic acid in the 6th position of the beta chain
Question 13: Which of the following hemoglobins is insoluble under lowered oxygen tension?
Answer: Hb-S
Question 14: What laboratory test is used to confirm the specific diagnosis of sickle cell anemia?
Answer: Hemoglobin electrophoresis
Question 15: In the solubility test for hemoglobin S, what is the reagent responsible for the reduction of
the hemoglobin molecule?
Answer: Sodium dithionite
Question 16: In hemoglobin C, what replaces glutamic acid?
Answer: Lysine
Question 17: Which red blood cell inclusion is characteristic of hemoglobin C disease?
Answer: Rod-shaped crystals
Question 18: What is hemoglobin F composed of?
Answer: 2 alpha and 2 gamma chains
Question 19: Which hemoglobin is resistant to alkali denaturation?
Answer: Hb-F
Page 2
, Question 20: Which hemoglobins are differentiated by the Kleihauer-Betke technique?
Answer: Hb-A1 from Hb-F
Question 21: What is the order of migration of hemoglobins, from fastest to slowest, on cellulose acetate
at pH 8.4?
Answer: A1, F, S, C
Question 22: Why is citrate agar used when abnormal hemoglobins are identified on cellulose acetate at
pH 8.6?
Answer: It separates hemoglobin S from hemoglobin D
Question 23: The cyanmethemoglobin method measures all hemoglobin pigments EXCEPT which one?
Answer: Sulfhemoglobin
Question 24: What is the diluent used in the cyanmethemoglobin method for the determination of
hemoglobin?
Answer: Drabkin's solution
Question 25: What is the normal plasma hemoglobin concentration?
Answer: 2-3 mg/dL
Question 26: Which plasma protein binds free hemoglobin?
Answer: Haptoglobin
Question 27: Which one of the following statements concerning hemolytic anemia is false?
Answer: The serum haptoglobin is increased
Question 28: In this type of anemia RBCs are normocytic and normochromic. RBCs, hemoglobin, and
hematocrit are decreased and the indirect bilirubin is increased.
Answer: Hemolytic anemia
Question 29: Myoglobin can be separated from hemoglobin by saturating the specimen with which one
of the following reagents?
Answer: Ammonium sulfite
Page 3
