BIO 100 M1 LAB ASSIGNMENT ACT II
SCIENTIFIC REASONING COMPLETE ANALYSIS
WITH ACCURATE ANSWERS
◉ What does PCR stand for?
Answer: Polymerase chain reaction
◉ Which of the following will be components in your PCR mixture?
Answer: Master mix
Human DNA
◉ What are you trying to amplify using PCR in this experiment?
Answer: Short tandem repeats from specific genes
◉ Match the definitions with the PCR terminology.
Denaturation
Annealing
Extension
,Thermocycler
Answer: Denaturation: The DNA template is heated so the strands
separate.
Annealing: The reaction is cooled so the primers can stick to the
DNA template.
Extension: The temperature is changed to the ideal temperature for
Taq polymerase to build (synthesize) a new strand based on the
template.
Thermocycler:
The machine that controls the temperature of the reaction and has
been programmed to move through the heating and cooling steps.
◉ When you arrive at lab, you need to be wearing [a] shoes. When it
is time to start experiments, you must wear [b] and, to keep you safe,
they must remain over your [c] while [d] in the lab is doing
experiments.
Answer: When you arrive at lab, you need to be wearing close-toed
shoes. When it is time to start experiments, you must wear goggles
and, to keep you safe, they must remain over your eyes while anyone
in the lab is doing experiments.
, ◉ Match the function to the name of the lab equipment.
Centrifuge
Thermocycler
Micropipette
Chelex beads
Answer: Centrifuge: Spins at a high speed to separate things by
density (denser stuff moves lower in the tube).
Thermocycler: Can be programmed to keep samples/reactions at
specific temperatures for specific lengths of time.
Micropipette: Measures and transfers very specific, usually small,
volumes of fluid accurately.
Chelex beads: Bind metal ions so they can be removed from the
sample.
◉ [x] uses a/an [y] called Taq polymerase to create [z] of copies of a
specific region of [w].
SCIENTIFIC REASONING COMPLETE ANALYSIS
WITH ACCURATE ANSWERS
◉ What does PCR stand for?
Answer: Polymerase chain reaction
◉ Which of the following will be components in your PCR mixture?
Answer: Master mix
Human DNA
◉ What are you trying to amplify using PCR in this experiment?
Answer: Short tandem repeats from specific genes
◉ Match the definitions with the PCR terminology.
Denaturation
Annealing
Extension
,Thermocycler
Answer: Denaturation: The DNA template is heated so the strands
separate.
Annealing: The reaction is cooled so the primers can stick to the
DNA template.
Extension: The temperature is changed to the ideal temperature for
Taq polymerase to build (synthesize) a new strand based on the
template.
Thermocycler:
The machine that controls the temperature of the reaction and has
been programmed to move through the heating and cooling steps.
◉ When you arrive at lab, you need to be wearing [a] shoes. When it
is time to start experiments, you must wear [b] and, to keep you safe,
they must remain over your [c] while [d] in the lab is doing
experiments.
Answer: When you arrive at lab, you need to be wearing close-toed
shoes. When it is time to start experiments, you must wear goggles
and, to keep you safe, they must remain over your eyes while anyone
in the lab is doing experiments.
, ◉ Match the function to the name of the lab equipment.
Centrifuge
Thermocycler
Micropipette
Chelex beads
Answer: Centrifuge: Spins at a high speed to separate things by
density (denser stuff moves lower in the tube).
Thermocycler: Can be programmed to keep samples/reactions at
specific temperatures for specific lengths of time.
Micropipette: Measures and transfers very specific, usually small,
volumes of fluid accurately.
Chelex beads: Bind metal ions so they can be removed from the
sample.
◉ [x] uses a/an [y] called Taq polymerase to create [z] of copies of a
specific region of [w].
