PCB 3023L Midterm PCB 3063 Florida State University | UPDATED
Questions with 100% Verified Answers
Question:
Types of Pipetteman
Answer:
1. P20 (1-20 ul)
2. P200 (20-200 ul)
3. P1000 (200-100 ul)
Question:
Advantage of small volumes in lab
Answer:
Minimizing use of costly agents and offers the possibility of automation
Question:
Disposable polypropylene conical centrifuge tubes
Answer:
All biochemical research and molecular biological research is conducted in these
Question:
Cell biology
Answer:
The analysis of cellular function: proteins, enzymes, nucleic acids
Question:
Purpose of a standard curve
Answer:
A series of samples that have known concentration of protein and can be used to
determine the concentrations of unknown samples
Question:
Requirements of a standard curve
, Answer:
1. Pure sample of known concentration using a standard and bovine serum albumen
(BSA)
2. Method to assess the samples being measured such as a UV or Visible light
spectrophotometer
Question:
Components of a standard curve graph
Answer:
1. x axis: protein BSA in ug
2. y axis: OD at 595 nm
3. y=mx+b where m=slope, b=y-intercept, y=OD, x=protein (unknown)
Question:
Spectrophotometry
Answer:
A technique used to measure the amount of a particular compound in a solution based
on the amount of light of a specific wavelength that is absorbed as the light passes
through the solution
Question:
Mechanics of a spectophotometer
Answer:
1. light source
2. prism
3. light wavelengths
4. monochometer
5. sample
6. photodetector
7. potentiometer
Question:
The effect of the amount of molecules in a sample to the
absorbance
Questions with 100% Verified Answers
Question:
Types of Pipetteman
Answer:
1. P20 (1-20 ul)
2. P200 (20-200 ul)
3. P1000 (200-100 ul)
Question:
Advantage of small volumes in lab
Answer:
Minimizing use of costly agents and offers the possibility of automation
Question:
Disposable polypropylene conical centrifuge tubes
Answer:
All biochemical research and molecular biological research is conducted in these
Question:
Cell biology
Answer:
The analysis of cellular function: proteins, enzymes, nucleic acids
Question:
Purpose of a standard curve
Answer:
A series of samples that have known concentration of protein and can be used to
determine the concentrations of unknown samples
Question:
Requirements of a standard curve
, Answer:
1. Pure sample of known concentration using a standard and bovine serum albumen
(BSA)
2. Method to assess the samples being measured such as a UV or Visible light
spectrophotometer
Question:
Components of a standard curve graph
Answer:
1. x axis: protein BSA in ug
2. y axis: OD at 595 nm
3. y=mx+b where m=slope, b=y-intercept, y=OD, x=protein (unknown)
Question:
Spectrophotometry
Answer:
A technique used to measure the amount of a particular compound in a solution based
on the amount of light of a specific wavelength that is absorbed as the light passes
through the solution
Question:
Mechanics of a spectophotometer
Answer:
1. light source
2. prism
3. light wavelengths
4. monochometer
5. sample
6. photodetector
7. potentiometer
Question:
The effect of the amount of molecules in a sample to the
absorbance
