2026-2027 Updated.
Bacteriophage - Answer Viruses that infect bacterial cells
Bacteriophages are... - Answer Obligate intracellular parasites
What do bacteriophages require? - Answer A host
Why do bacteriophages require hosts? - Answer They lack specific requirements for replication
and reproduction - use host cell machinery
What phages infect E. coli B? - Answer T2 and T4
What phage infects E. coli K12? - Answer T4
Why is phage typing important? - Answer Can help determine infectious strains within a
species of bacteria
Phage therapy has been used for what? - Answer To treat antibiotic resistance
What encases the nucleic acid in bacteriophages? - Answer Nucleocapsid
Protein sheath has ___________________ to inject its DNA or RNA into the cell - Answer
contractile mechanisms
T/F: Do bacteriophages have a hollow core in their sheaths? - Answer True
What is a base plate made of, and what are they used for? - Answer Tail fibers that are used
for attaching to cells
What does the spike do? - Answer Penetrates the cell wall
What are the steps of the lytic cycle? - Answer - Phage injects its DNA/RNA
- Bacterial cell's DNA is hydrolyzed
- Bacterial cell's machinery produces the components of phage virions
, - Phage causes the production of lysozyme that lyses the bacterial cell
What is burst size? - Answer Amount of virions released per cell lysis
What causes plaques? - Answer If the phage is applied to a bacterial lawn that is susceptible to
it
What are plaques? - Answer Clearing in the agar
What is fermentation? - Answer A means of food preservation due to the acids and alcohols
produced by some microbes
What is fermentation of wine carried out by? - Answer Saccharomyces cerevisiae var.
ellipsoideus
As the _________ consumes the simple sugars in grape juice, they are broken down for energy,
and the _____________ produces _____________ and ________________________. - Answer
Yeast; yeast; ethanol and carbon dioxide
Psychrophiles - Answer -5 to 20 degrees Celsius
Mesophiles - Answer 20 to 50 degrees Celsius
Thermophiles - Answer 50 to 80 degrees Celsius (soil bacteria)
Hyperthermophiles - Answer Above 80 degrees Celsius
What are psychrotrophs? - Answer Cold tolerant bacteria (ex. Listeria)
What produces a red pigment called prodigiosin? - Answer Serratia marcescens
What properties does prodigiosin exhibit? - Answer Antibacterial and anti-tumor properties
What reagent do you use for the Indole production test of SIM? - Answer Kovac's reagent
How long does the VP test usually take? - Answer 30 minutes