BIOL 3201 Lab Practical Questions and
All Correct Answers 2026-2027 Updated.
where are gloves disposed? - Answer biohazard can
where should slides/swabs be disposed? - Answer sharps container
where are tubes disposed? - Answer discard cart
where are plates disposed? - Answer biohazard can
Biosafety Level 1 (BSL-1) - Answer microorganisms not known to cause disease in healthy
adults
PPE (personal protective equipment) is NOT required for this BSL - Answer BSL 1
Biosafety level 2 - Answer indigenous microorganisms that can lead to diseases of varying
severity in healthy adults
PPE must be worn including lab coats and gloves. eye protection and face shields are optional
for this BSL - Answer BSL 2
Biosafety level 3 - Answer indigenous or exotic microorganisms that cause serious or
potentially lethal disease through respiratory transmission
PPE must be worn for this BSL including lab coats, gloves, eye protection, and respirators -
Answer BSL 3
Biosafety level 4 - Answer microorganisms that are dangerous and exotic with high risk of
aerosol transmitted infections. rarely are there treatments or vaccines for these
microorganisms, and the disease they cause are frequently fatal
This BSL requires a Class III biosafety cabinet or a fully body positive pressure suit to be worn -
Answer BSL 4
microorganism - Answer living organisms that cannot be seen without a microscope.
, ubiquity - Answer a term meaning microorganisms are everywhere
what is used in order to prevent contamination from microorganisms? - Answer aseptic
technique
This can destroy vegetative cells and viruses but may not destroy endospores - Answer
disinfectant
what is used to clean work area before and after work? - Answer lysol
what are loops and needles used for? - Answer to transfer cultures and to streak or inoculate
petri plates with bacteria
when should you flame your loop? - Answer before and after inoculation
how long should you let the loop cool after flaming? - Answer 15-20 seconds
what side of the plate should you label? - Answer Bottom side (agar side)
brightfield - Answer simplest form of microscopy where light is either passed through, or
reflected off, a specimen. Requires the use of stains to visualize cells
darkfield - Answer contrast is created by a bright specimen on a dark background. ideal for
external, not great for internal structures. stains not required.
fluorescent - Answer uses high intensity illumination to excite fluorescent molecules
(fluorophores) in the samples. requires the use of fluorophores
what type of microscopy allows for the visualization of morphology, external structures, and
some internal structures? - Answer phase contrast
what type of microscope is ideal for revealing morphology and external structures but doesn't
provide a great deal of information about internal structure? - Answer darkfield
Phase contrast - Answer converts the differences in optical density (refractive index) of cells
into different shades of brightness (contrast). stains not required
framework - Answer structure of microscope and composed of the arm and base
All Correct Answers 2026-2027 Updated.
where are gloves disposed? - Answer biohazard can
where should slides/swabs be disposed? - Answer sharps container
where are tubes disposed? - Answer discard cart
where are plates disposed? - Answer biohazard can
Biosafety Level 1 (BSL-1) - Answer microorganisms not known to cause disease in healthy
adults
PPE (personal protective equipment) is NOT required for this BSL - Answer BSL 1
Biosafety level 2 - Answer indigenous microorganisms that can lead to diseases of varying
severity in healthy adults
PPE must be worn including lab coats and gloves. eye protection and face shields are optional
for this BSL - Answer BSL 2
Biosafety level 3 - Answer indigenous or exotic microorganisms that cause serious or
potentially lethal disease through respiratory transmission
PPE must be worn for this BSL including lab coats, gloves, eye protection, and respirators -
Answer BSL 3
Biosafety level 4 - Answer microorganisms that are dangerous and exotic with high risk of
aerosol transmitted infections. rarely are there treatments or vaccines for these
microorganisms, and the disease they cause are frequently fatal
This BSL requires a Class III biosafety cabinet or a fully body positive pressure suit to be worn -
Answer BSL 4
microorganism - Answer living organisms that cannot be seen without a microscope.
, ubiquity - Answer a term meaning microorganisms are everywhere
what is used in order to prevent contamination from microorganisms? - Answer aseptic
technique
This can destroy vegetative cells and viruses but may not destroy endospores - Answer
disinfectant
what is used to clean work area before and after work? - Answer lysol
what are loops and needles used for? - Answer to transfer cultures and to streak or inoculate
petri plates with bacteria
when should you flame your loop? - Answer before and after inoculation
how long should you let the loop cool after flaming? - Answer 15-20 seconds
what side of the plate should you label? - Answer Bottom side (agar side)
brightfield - Answer simplest form of microscopy where light is either passed through, or
reflected off, a specimen. Requires the use of stains to visualize cells
darkfield - Answer contrast is created by a bright specimen on a dark background. ideal for
external, not great for internal structures. stains not required.
fluorescent - Answer uses high intensity illumination to excite fluorescent molecules
(fluorophores) in the samples. requires the use of fluorophores
what type of microscopy allows for the visualization of morphology, external structures, and
some internal structures? - Answer phase contrast
what type of microscope is ideal for revealing morphology and external structures but doesn't
provide a great deal of information about internal structure? - Answer darkfield
Phase contrast - Answer converts the differences in optical density (refractive index) of cells
into different shades of brightness (contrast). stains not required
framework - Answer structure of microscope and composed of the arm and base
