BIOL 3201 Final Exam 2 Questions and
All Solved Solutions 2026-2027 Updated.
Methyl Red (MR) tests for what things? - Answer lactic, acetic, succinic, and formic acids
produced by various G- genera
Methyl Red lowers the pH to ____ or _____. - Answer 5.0 or less.
Methyl Red Ph indicator turns what if acid is present? - Answer red
If the Methyl Red indicator turns red it is positive for what ability? - Answer the ability to
produce and maintain stable acid end products from glucose fermentation
Voges-Proskaur (VP) tests for what? - Answer butandiol fermentation.
Voges-Proskaur (VP) indicates the fermentation of ________ which produces a natural end
product of 2, ______________. - Answer Glucose; 3-butanedoil
Voges-Proskaur's (VP)end product is converted to what? Which reacts with VP1 and VP2
(******added after 3-5 days******). - Answer acetoin
SIM stand for what? - Answer Sulfide, Indole, Motility
Sulfide is the reduction of what element? - Answer sulfur to H2S
SIM medium contains what and what? - Answer ferrous ammonium sulfate and sodium
thiosulfate
In SIM H2S production interacts with these compounds(ferrous ammonium sulfate and sodium
thiosulfate), forming a ________ precipitate. - Answer black
Does the Sulfide in SIM use a reagent, if so which one? - Answer no reagent used for the
sulfide test
Does Indole Production in SIM use a reagent, if so which one? - Answer yes and Kovac's
reagent
, Indole Production is the ability to degrade tryptophan to produce indole. T/F - Answer True
A positive Indole Production turns what when Kovac's Reagent is added? - Answer red
Does the test tube change color if it is negative for Indole production? - Answer no color
change
In the motility test of SIM is there a reagent used, if so what reagent? - Answer no reagent is
used in motility
With a positive motility test the tube will show what? - Answer turbidity
With a negative motility test the tube with show what? - Answer growth limited to the stab
line
In the citrate test, it uses CITRATE as the sole carbon source and AMMONIUM SALTS as nitrogen
source. T/F - Answer true
In the citrate test production of what causes the medium to become alkaline? - Answer
Ammonia
For the Citrate test what is the pH indicator? - Answer Bromothymol blue
In Citrate the pH indicator in the medium turns from dark green to what when positive? -
Answer blue
IMViC stands for what? - Answer Indole, Methyl red, Voges-Proskauer, Citrate
What is the first tests you should do after determining a bacteria to be in the
Enterobacteriaceae family? - Answer IMViC
The IMViC test usually narrows the 7 organisms down to how many? - Answer 2
For the IMViC tests how many tubes will you use? - Answer 3 tubes (one for each test: SIM,
MRVP, CITRATE)
How many tests is the IMViC? - Answer 6 tests
All Solved Solutions 2026-2027 Updated.
Methyl Red (MR) tests for what things? - Answer lactic, acetic, succinic, and formic acids
produced by various G- genera
Methyl Red lowers the pH to ____ or _____. - Answer 5.0 or less.
Methyl Red Ph indicator turns what if acid is present? - Answer red
If the Methyl Red indicator turns red it is positive for what ability? - Answer the ability to
produce and maintain stable acid end products from glucose fermentation
Voges-Proskaur (VP) tests for what? - Answer butandiol fermentation.
Voges-Proskaur (VP) indicates the fermentation of ________ which produces a natural end
product of 2, ______________. - Answer Glucose; 3-butanedoil
Voges-Proskaur's (VP)end product is converted to what? Which reacts with VP1 and VP2
(******added after 3-5 days******). - Answer acetoin
SIM stand for what? - Answer Sulfide, Indole, Motility
Sulfide is the reduction of what element? - Answer sulfur to H2S
SIM medium contains what and what? - Answer ferrous ammonium sulfate and sodium
thiosulfate
In SIM H2S production interacts with these compounds(ferrous ammonium sulfate and sodium
thiosulfate), forming a ________ precipitate. - Answer black
Does the Sulfide in SIM use a reagent, if so which one? - Answer no reagent used for the
sulfide test
Does Indole Production in SIM use a reagent, if so which one? - Answer yes and Kovac's
reagent
, Indole Production is the ability to degrade tryptophan to produce indole. T/F - Answer True
A positive Indole Production turns what when Kovac's Reagent is added? - Answer red
Does the test tube change color if it is negative for Indole production? - Answer no color
change
In the motility test of SIM is there a reagent used, if so what reagent? - Answer no reagent is
used in motility
With a positive motility test the tube will show what? - Answer turbidity
With a negative motility test the tube with show what? - Answer growth limited to the stab
line
In the citrate test, it uses CITRATE as the sole carbon source and AMMONIUM SALTS as nitrogen
source. T/F - Answer true
In the citrate test production of what causes the medium to become alkaline? - Answer
Ammonia
For the Citrate test what is the pH indicator? - Answer Bromothymol blue
In Citrate the pH indicator in the medium turns from dark green to what when positive? -
Answer blue
IMViC stands for what? - Answer Indole, Methyl red, Voges-Proskauer, Citrate
What is the first tests you should do after determining a bacteria to be in the
Enterobacteriaceae family? - Answer IMViC
The IMViC test usually narrows the 7 organisms down to how many? - Answer 2
For the IMViC tests how many tubes will you use? - Answer 3 tubes (one for each test: SIM,
MRVP, CITRATE)
How many tests is the IMViC? - Answer 6 tests
