BIOL 3201 Midterm (Auburn) Exam with
Complete Answers 2026/2027 Update.
Gloves to be disposed of where? - Answer biohazard can
Slides/swabs to be disposed of where? - Answer sharps container
Tubes to be disposed of where? - Answer discard cart
Plates to be disposed of where? - Answer biohazard can
BSL 1 - Answer microorganisms not known to cause disease in healthy adults
BSL 2 - Answer indigenous microorganisms that can lead to diseases of varying severity in
healthy adults
BSL 3 - Answer indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission
BSL 4 - Answer microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections; rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal
Ubiquity - Answer a term meaning microorganisms are everywhere
What is used in order to prevent contamination from microorganisms? - Answer aseptic
technique
Disinfectants - Answer can destroy vegetative cells and viruses but may not destroy
endospores
What is used to clean work area before and after work? - Answer lysol
How long should you let the loop cool after flaming? - Answer 10-15 seconds
What side of the plate should you label - Answer bottom/agar side
, Brightfield - Answer simplest form of microscopy where light is either passed through, or
reflected off, a specimen; requires the use of stains to visualize cells
Darkfield - Answer contrast is created by a bright specimen on a dark background; ideal for
external, not great for internal structures; stains not required
Fluorescent - Answer uses high intensity illumination to excite fluorescent molecules
(fluorophores) in the sample
Framework - Answer structure of the microscope and composed of the arm and base
Stage - Answer horizontal area that supports the microscope slide
Oculars - Answer eyepieces that typically provide magnification of 10x
Stage adjustment knobs - Answer allow for the holding and moving of the slide around the
area that supports the slide
Condenser - Answer collects and directs the light from the light source to the slide and is
located under the are that supports the slide
Objectives - Answer attached to a rotating nosepiece; have powers of 10x, 40x, 100x
Which lens use immersion oil? - Answer 100x
Diaphragm - Answer controls the amount of light that reaches the slide
Coarse focus knob - Answer used first to bring objects on slide into focus
Fine focus knob - Answer after moving to 40x and 100x, this is used to bring objects on slide
into focus
Numerical aperture - Answer a mathematical expression that describes how the condenser
lens concentrates and focuses the light rays from the light source
Complete Answers 2026/2027 Update.
Gloves to be disposed of where? - Answer biohazard can
Slides/swabs to be disposed of where? - Answer sharps container
Tubes to be disposed of where? - Answer discard cart
Plates to be disposed of where? - Answer biohazard can
BSL 1 - Answer microorganisms not known to cause disease in healthy adults
BSL 2 - Answer indigenous microorganisms that can lead to diseases of varying severity in
healthy adults
BSL 3 - Answer indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission
BSL 4 - Answer microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections; rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal
Ubiquity - Answer a term meaning microorganisms are everywhere
What is used in order to prevent contamination from microorganisms? - Answer aseptic
technique
Disinfectants - Answer can destroy vegetative cells and viruses but may not destroy
endospores
What is used to clean work area before and after work? - Answer lysol
How long should you let the loop cool after flaming? - Answer 10-15 seconds
What side of the plate should you label - Answer bottom/agar side
, Brightfield - Answer simplest form of microscopy where light is either passed through, or
reflected off, a specimen; requires the use of stains to visualize cells
Darkfield - Answer contrast is created by a bright specimen on a dark background; ideal for
external, not great for internal structures; stains not required
Fluorescent - Answer uses high intensity illumination to excite fluorescent molecules
(fluorophores) in the sample
Framework - Answer structure of the microscope and composed of the arm and base
Stage - Answer horizontal area that supports the microscope slide
Oculars - Answer eyepieces that typically provide magnification of 10x
Stage adjustment knobs - Answer allow for the holding and moving of the slide around the
area that supports the slide
Condenser - Answer collects and directs the light from the light source to the slide and is
located under the are that supports the slide
Objectives - Answer attached to a rotating nosepiece; have powers of 10x, 40x, 100x
Which lens use immersion oil? - Answer 100x
Diaphragm - Answer controls the amount of light that reaches the slide
Coarse focus knob - Answer used first to bring objects on slide into focus
Fine focus knob - Answer after moving to 40x and 100x, this is used to bring objects on slide
into focus
Numerical aperture - Answer a mathematical expression that describes how the condenser
lens concentrates and focuses the light rays from the light source
