BIOL 3201 Final exam With Guaranteed
Pass Solutions 2026-2027 Edition.
BSL 1 - Answer microorganism not known to cause disease in healthy adults
-standard microbiological practices followed
-personal protective equipment not required
-sink available for hand washing
BSL 2 - Answer Indigenous microorganisms that can lead to diseases of varying severity in
healthy adults.
-contaminated waste is autoclaved
-PPE must be worn
BSL 3 - Answer Indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission
BSL 4 - Answer Microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal.
microorganism - Answer general term for living organism that cannot be seen without a
microscope (even though some people include viruses and prions in the group)
ubiquity of microorganisms - Answer refers to the concept that microorganisms are
everywhere
-not entirely true but microorganisms do occupy an amazing range of environments
microbes - Answer _______ are found in air we breathe, food we eat, water we drink
labeling plates - Answer 1. TSA (kind of medium)
2.first initial and full last name
3. date
4. lab day / time
5. incubation temperature
labeling the agar side - Answer 1. will always know the identity of the plate even if the lid
becomes separated
, 2. you incubate plates agar side up , if condensation occurs it will develop on the lid not the agar
surface
why practice aseptic technique - Answer 1. contaminate others with microorganisms that
could lead to infection
2. contaminate themselves with microorganisms which leads to infection
3. contaminate media and / or microorganisms being studied in the lab
disinfectants - Answer can destroy vegetative cells and viruses but may not destroy
endospores
-cannot be used to sterilize surfaces or equipment
-ex: Lysol , 70% ethanol , 10% bleach , quarternary ammonia
flaming your loop / needle - Answer accomplished by placing the loop or needle into the
Bunsen Burner flame until red hot , entire loop or needle must be heated this way
-any contamination will be incinerated leaving it sterile
cool - Answer It is absolutely necessary to let the loop or needle ______ before transferring it
into a inoculum of bacteria
pinky finger - Answer how do you remove the cap of a tube before inserting a cooled loop /
needle?
Middleburg, Holland - Answer where was the first microscope invented?
brightfield microscope - Answer simplest form of microscopy, light is either passed through or
reflected off a specimen
-illumination is not altered by devices that change the properties of light
-requires the use of stains to visualize cells
phase contrast microscope - Answer converts the differences in optical density (refractive
index) of cells into different shades of brightness (contrast)
-visualization of morphology, external structures, some internal structures
-stains not required
darkfield microscope - Answer contrast is created by a bright specimen on a dark background
-ideal for revealing morphology and external structures
Pass Solutions 2026-2027 Edition.
BSL 1 - Answer microorganism not known to cause disease in healthy adults
-standard microbiological practices followed
-personal protective equipment not required
-sink available for hand washing
BSL 2 - Answer Indigenous microorganisms that can lead to diseases of varying severity in
healthy adults.
-contaminated waste is autoclaved
-PPE must be worn
BSL 3 - Answer Indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission
BSL 4 - Answer Microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal.
microorganism - Answer general term for living organism that cannot be seen without a
microscope (even though some people include viruses and prions in the group)
ubiquity of microorganisms - Answer refers to the concept that microorganisms are
everywhere
-not entirely true but microorganisms do occupy an amazing range of environments
microbes - Answer _______ are found in air we breathe, food we eat, water we drink
labeling plates - Answer 1. TSA (kind of medium)
2.first initial and full last name
3. date
4. lab day / time
5. incubation temperature
labeling the agar side - Answer 1. will always know the identity of the plate even if the lid
becomes separated
, 2. you incubate plates agar side up , if condensation occurs it will develop on the lid not the agar
surface
why practice aseptic technique - Answer 1. contaminate others with microorganisms that
could lead to infection
2. contaminate themselves with microorganisms which leads to infection
3. contaminate media and / or microorganisms being studied in the lab
disinfectants - Answer can destroy vegetative cells and viruses but may not destroy
endospores
-cannot be used to sterilize surfaces or equipment
-ex: Lysol , 70% ethanol , 10% bleach , quarternary ammonia
flaming your loop / needle - Answer accomplished by placing the loop or needle into the
Bunsen Burner flame until red hot , entire loop or needle must be heated this way
-any contamination will be incinerated leaving it sterile
cool - Answer It is absolutely necessary to let the loop or needle ______ before transferring it
into a inoculum of bacteria
pinky finger - Answer how do you remove the cap of a tube before inserting a cooled loop /
needle?
Middleburg, Holland - Answer where was the first microscope invented?
brightfield microscope - Answer simplest form of microscopy, light is either passed through or
reflected off a specimen
-illumination is not altered by devices that change the properties of light
-requires the use of stains to visualize cells
phase contrast microscope - Answer converts the differences in optical density (refractive
index) of cells into different shades of brightness (contrast)
-visualization of morphology, external structures, some internal structures
-stains not required
darkfield microscope - Answer contrast is created by a bright specimen on a dark background
-ideal for revealing morphology and external structures
