ACS Biochemistry Exam 2027 Questions and Answers | Verified
| A+
Question 1.
Buffer Order and Henderson Hasselbach
Correct Answer: Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease
as temp increase so pH decrease
Question 2.
Ramachandran plots
Correct Answer: Φ = N-R ψ = C-R optimal angle to prevent steric hinderance
Question 3.
Protein structure of a helix, B sheet, reverse turn
Correct Answer: a: LEMARKHQ B: VICYFTW RT: PSDNG
Question 4.
how many amino acids in alpha turn helix
Correct Answer: 3.6
Question 5.
Super secondary structure
Correct Answer: helix turn helix
Question 6.
parallel vs. antiparallel
Correct Answer: parallel wedges face same way antiparallel face opposite
Question 7.
Isoelectric point (pI)
Correct Answer: pH at which a particular molecule carries no net electrical
charge.
Question 8.
Protein Separation Techniques (ALL)
Correct Answer: Homogenization Salting Out Affinity Chromatgraphy Ion
Exchange Size Exclusion/Gel Filtration H, S, A, I, SG He said all individuals SinG
, Question 9.
Protein Separation Techniques (H)
Correct Answer: Homogenization disrupt cells, sonicator, centrifuge
• Protein Separation Techniques (S) -✓✓Salting out different proteins
precipitate out at different salt concentrations
Question 10.
Protein Separation Techniques (A)
Correct Answer: Affinity chromatography add a tag; histidine binds; see how
well there is an affinity for the group
Question 11.
Protein Separation Techniques (I)
Correct Answer: ion exchange separation based on charge; + proteins stick to
- beads
Question 12.
Protein Separation Techniques (SG)
Correct Answer: Size Exclusion/Gel Filtration separation based on size
Question 13.
Cuts (protein separation techniques)
Correct Answer: cut out what dont need/isnt protein (use 40% concen. then
keep liquid; if use 60% concen. then keep pellet)
Question 14.
Dialysis
Correct Answer: protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Question 15.
Electrophoresis
Correct Answer: gels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page pH separation is by pI and charge in 2d
| A+
Question 1.
Buffer Order and Henderson Hasselbach
Correct Answer: Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease
as temp increase so pH decrease
Question 2.
Ramachandran plots
Correct Answer: Φ = N-R ψ = C-R optimal angle to prevent steric hinderance
Question 3.
Protein structure of a helix, B sheet, reverse turn
Correct Answer: a: LEMARKHQ B: VICYFTW RT: PSDNG
Question 4.
how many amino acids in alpha turn helix
Correct Answer: 3.6
Question 5.
Super secondary structure
Correct Answer: helix turn helix
Question 6.
parallel vs. antiparallel
Correct Answer: parallel wedges face same way antiparallel face opposite
Question 7.
Isoelectric point (pI)
Correct Answer: pH at which a particular molecule carries no net electrical
charge.
Question 8.
Protein Separation Techniques (ALL)
Correct Answer: Homogenization Salting Out Affinity Chromatgraphy Ion
Exchange Size Exclusion/Gel Filtration H, S, A, I, SG He said all individuals SinG
, Question 9.
Protein Separation Techniques (H)
Correct Answer: Homogenization disrupt cells, sonicator, centrifuge
• Protein Separation Techniques (S) -✓✓Salting out different proteins
precipitate out at different salt concentrations
Question 10.
Protein Separation Techniques (A)
Correct Answer: Affinity chromatography add a tag; histidine binds; see how
well there is an affinity for the group
Question 11.
Protein Separation Techniques (I)
Correct Answer: ion exchange separation based on charge; + proteins stick to
- beads
Question 12.
Protein Separation Techniques (SG)
Correct Answer: Size Exclusion/Gel Filtration separation based on size
Question 13.
Cuts (protein separation techniques)
Correct Answer: cut out what dont need/isnt protein (use 40% concen. then
keep liquid; if use 60% concen. then keep pellet)
Question 14.
Dialysis
Correct Answer: protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Question 15.
Electrophoresis
Correct Answer: gels - move by size with electric current
1. Isoelectric focusing - purely pH
2. SDS page pH separation is by pI and charge in 2d
