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  • Universiteit Antwerpen (UA)
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  • Analytische biochemie I
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Samenvatting Formularium Analytische Biochemie I | BMW | UA | 2026/27

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2025/2026 Written for academic year
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Universiteit Antwerpen (UA)
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Analytische biochemie I
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Dit formularium voor Analytische Biochemie I van Zeno De Paus bevat alle essentiële formules en constanten voor het vak bij de Universiteit Antwerpen. Het document behandelt zes hoofdstukken: inleiding tot analytische biochemie, celcultuur en celfractionatie, filtratie en precipitatie, centrifugatie, chromatografie, en elektroforese, met alle relevante berekeningsmethoden. Onmisbaar voor examens en praktische opgaven - bespaard tijd bij het zoeken naar formules en biedt een overzichtelijke referentie voor alle kernconcepten van het vak.

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Zeno De Paus Formularium: Analytische biochemie 1 2de Bach. BMW
Hoofdstuk 1: Inleiding tot analytische biochemie
𝑥 𝑔 𝑠𝑡𝑜𝑓
Weight per volume %: 𝑥% (𝑤/𝑣) = 100 𝑚𝑙 𝑜𝑝𝑙𝑜𝑠𝑠𝑖𝑛𝑔

𝑥 𝑔 𝑠𝑡𝑜𝑓
Weight per weight %: 𝑥% (𝑤/𝑤) = 100 𝑔 𝑠𝑡𝑜𝑐𝑘

𝑥 𝑔 𝑜𝑝𝑙𝑜𝑠𝑠𝑖𝑛𝑔
Volume per volume %: 𝑥% (𝑣/𝑣) = 100 𝑚𝑙 𝑜𝑝𝑙𝑜𝑠𝑠𝑖𝑛𝑔

𝑥 𝑐𝑚3 𝑠𝑡𝑜𝑓
Parts per million (ppm): 𝑥 𝑝𝑝𝑚 = 1 000 000 𝑐𝑚3

𝑥 𝑐𝑚3 𝑠𝑡𝑜𝑓
Parts per billion (ppb): 𝑥 𝑝𝑝𝑏 = 1 000 000 000 (!!! Billion = milliard in NL)
𝑛
Molariteit: 𝐶 = 𝑉
𝑚 𝑁
Molariteit: 𝑛 = 𝑀𝑀 = 𝑁
𝐴


Verdunningsreeks: 𝐶1 𝑉1 = 𝐶2 𝑉2
𝑚
Dichtheid: 𝜌 = 𝑉

1
Ionische sterkte: 𝜇 = 2 ∑ 𝑐𝑛 𝑧𝑛2

Activiteit: 𝐴𝑥 = 𝛾𝑥 [𝑋]

Hoofdstuk 2: Celcultuur en celfractionatie

Groeikinetiek van cellen: 𝑁(𝑡) = 𝑁0 . 2𝑘𝑡
𝑙𝑒𝑒𝑓𝑏𝑎𝑟𝑒 𝑐𝑒𝑙𝑙𝑒𝑛 𝑜𝑛𝑔𝑒𝑘𝑙𝑒𝑢𝑟𝑑𝑒 𝑐𝑒𝑙𝑙𝑒𝑛 𝑖𝑛 𝑛 𝑣𝑖𝑒𝑟𝑘𝑎𝑛𝑡𝑒𝑛
Aantal leefbare cellen: = . 𝑣𝑟𝑑𝑛𝑔𝑠𝑓𝑐𝑡𝑟 . 104
𝑚𝑙 𝑛

𝑑𝑝𝑣𝑔𝑒𝑚 𝑅𝑒 < 2300 → 𝑆𝑡𝑟𝑜𝑚𝑖𝑛𝑔 𝑖𝑠 𝑙𝑎𝑚𝑖𝑛𝑎𝑖𝑟
Reynoldsgetal: 𝑅𝑒 = →{
𝜂 𝑅𝑒 > 2300 → 𝑆𝑡𝑟𝑜𝑚𝑖𝑛𝑔 𝑘𝑎𝑛 𝑡𝑢𝑟𝑏𝑢𝑙𝑒𝑛𝑡 𝑧𝑖𝑗𝑛

Hoofdstuk 3: Filtratie en precipitatie
𝐶𝑓
ln( ) 𝑅 ≈ 1 → 𝐼𝑑𝑒𝑎𝑎𝑙 𝑚𝑒𝑚𝑏𝑟𝑎𝑎𝑛
𝐶𝑜
Selectiviteit van een (ultrafiltratie) membraan: 𝑅 = →{
𝑉
ln( 0 )
𝑅 ≈ 0 → 𝑁𝑖𝑒𝑡 − 𝑠𝑒𝑙𝑒𝑐𝑡𝑖𝑒𝑓
𝑉𝑓




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