HISTOTECHNOLOGY EXAM STUDY GUIDE WITH VERIFIED
QUESTIONS CORRECT ANSWERS | 2026/2027 LATEST UPDATE |
ALREADY GRADED A+
Borrelia burgdorferi organisms are demonstrated well with this procedure:
Fite
Gridley
Steiner
auramin -
ANSWER
Steiner
A major disadvantage in the use of osmium tetroxide as a fixative is its:
destruction of tissue lipids
tendency to interfere with staining
hardening effect on tissue
coagulation of both nuclear and cytoplasmic proteins. -
ANSWER
tendency to interfere with staining
Select the solutions used to "blue" the nuclei in tissue sections after a hematoxylin stain procedure.
More than one
ANSWER
may be correct.
Ammonium water
Acetic acid in alcohol
Scott solution
Lithium carbonate -
ANSWER
Ammonium water
,Scott solution
Lithium carbonate
A question on a quiz requires the candidate to calculate the change in temperature from Celsius to
Fahrenheit. The question is considered which of the following taxonomy levels?
1
2
3
4-
ANSWER
2
Amebas can be demonstrated with H&E stain and the:
Grocott methenamine silver procedure
Kinyoun acid-fast procedure
Vassar-Culling procedure
periodic acid-Schiff procedure -
ANSWER
periodic acid-Schiff procedure
The elastic stain, Aldehyde Fuchsin, will stain these other tissue elements. Select all that apply.
Acidic mucosubstances
Beta cell granules
Collagen
Sulfated mucosubstances -
ANSWER
Beta cell granules
Sulfated mucosubstances
, A good fixation is one that
makes tissues more permeable to subsequent reagents
preserves tissues in a manner which is not life-like
enhances putrefaction
does not harden tissues quickly -
ANSWER
makes tissues more permeable to subsequent reagents
This staining procedure is probably the most appropriate method for demonstrating acid-fast
organisms that are difficult to stain by other methods
Ziehl-Neelsen
Gridley
Kinyoun
Wade -
ANSWER
Wade
Which of the following special stains demonstrates amyloid deposits in tissue resulting in green
birefringence when viewed with a polarized lens?
Thioflavine T
Congo Red
Movat Pentachrome
Crystal Violet -
ANSWER
Congo Red
Application of these nonenzymatic (classic) staining procedures can be used in the diagnosis of
muscle disease. Select ALL that apply:
QUESTIONS CORRECT ANSWERS | 2026/2027 LATEST UPDATE |
ALREADY GRADED A+
Borrelia burgdorferi organisms are demonstrated well with this procedure:
Fite
Gridley
Steiner
auramin -
ANSWER
Steiner
A major disadvantage in the use of osmium tetroxide as a fixative is its:
destruction of tissue lipids
tendency to interfere with staining
hardening effect on tissue
coagulation of both nuclear and cytoplasmic proteins. -
ANSWER
tendency to interfere with staining
Select the solutions used to "blue" the nuclei in tissue sections after a hematoxylin stain procedure.
More than one
ANSWER
may be correct.
Ammonium water
Acetic acid in alcohol
Scott solution
Lithium carbonate -
ANSWER
Ammonium water
,Scott solution
Lithium carbonate
A question on a quiz requires the candidate to calculate the change in temperature from Celsius to
Fahrenheit. The question is considered which of the following taxonomy levels?
1
2
3
4-
ANSWER
2
Amebas can be demonstrated with H&E stain and the:
Grocott methenamine silver procedure
Kinyoun acid-fast procedure
Vassar-Culling procedure
periodic acid-Schiff procedure -
ANSWER
periodic acid-Schiff procedure
The elastic stain, Aldehyde Fuchsin, will stain these other tissue elements. Select all that apply.
Acidic mucosubstances
Beta cell granules
Collagen
Sulfated mucosubstances -
ANSWER
Beta cell granules
Sulfated mucosubstances
, A good fixation is one that
makes tissues more permeable to subsequent reagents
preserves tissues in a manner which is not life-like
enhances putrefaction
does not harden tissues quickly -
ANSWER
makes tissues more permeable to subsequent reagents
This staining procedure is probably the most appropriate method for demonstrating acid-fast
organisms that are difficult to stain by other methods
Ziehl-Neelsen
Gridley
Kinyoun
Wade -
ANSWER
Wade
Which of the following special stains demonstrates amyloid deposits in tissue resulting in green
birefringence when viewed with a polarized lens?
Thioflavine T
Congo Red
Movat Pentachrome
Crystal Violet -
ANSWER
Congo Red
Application of these nonenzymatic (classic) staining procedures can be used in the diagnosis of
muscle disease. Select ALL that apply:
