Biochemistry ACS Exam | Actual Questions and Answers Latest
Updated 2026/2027 (Graded A+)
• Question
Glycolysis
Correct Answer: Near-universal pathway in which glucose molecule is oxidized to two
molecules of pyruvate, with energy conserved as ATP and NADH
• Question
Glycolytic breakdown
Correct Answer: Glucose is the sole source of metabolic energy in some mammalian
tissues and cell types
• Question
Fermentation
Correct Answer: general form for the anaerobic degradation of glucose or other organic
nutrients to obtain energy, conserved as ATP
• Question
Preparatory phase
Correct Answer: ATP is invested to convert glucose to fructose 1,6-bisphosphate.
C3 & C4 broken to yield two molecules of triose phosphate.
• Question
Glycolysis: Preparatory phase
Correct Answer: 1. Glucose phosphorylated at OH on C-6, ATP used *hexokinase*
2. glucose 6-phosphate thus formed is converted to fructose 6-phosphate.
*phosphohexose isomerase*
3. Fructose 6-phosphate is phorylated at C-1 to yield Fructose
1,6-bisphosphate, ATP used *PFK-1*
4. Fructose 6-phosphate splits to 2 three carbon molecules, DHAP and GAP
*aldolase*
5. DHAP isomerize to GAP
*triose phosphate isomerase* (2 GAP molecules)
Updated 2026/2027 (Graded A+)
• Question
Glycolysis
Correct Answer: Near-universal pathway in which glucose molecule is oxidized to two
molecules of pyruvate, with energy conserved as ATP and NADH
• Question
Glycolytic breakdown
Correct Answer: Glucose is the sole source of metabolic energy in some mammalian
tissues and cell types
• Question
Fermentation
Correct Answer: general form for the anaerobic degradation of glucose or other organic
nutrients to obtain energy, conserved as ATP
• Question
Preparatory phase
Correct Answer: ATP is invested to convert glucose to fructose 1,6-bisphosphate.
C3 & C4 broken to yield two molecules of triose phosphate.
• Question
Glycolysis: Preparatory phase
Correct Answer: 1. Glucose phosphorylated at OH on C-6, ATP used *hexokinase*
2. glucose 6-phosphate thus formed is converted to fructose 6-phosphate.
*phosphohexose isomerase*
3. Fructose 6-phosphate is phorylated at C-1 to yield Fructose
1,6-bisphosphate, ATP used *PFK-1*
4. Fructose 6-phosphate splits to 2 three carbon molecules, DHAP and GAP
*aldolase*
5. DHAP isomerize to GAP
*triose phosphate isomerase* (2 GAP molecules)
