Practice Questions and Answers | HESI
A2 Biology Review | Rationales and
Complete Exam Preparation | Latest
2026/2027 Update
Description
This comprehensive HESI A2 Biology Exam Practice Set contains 200 multiple-choice
questions (MCQs) with correct answers marked with ✅ and detailed rationales for
complete exam preparation. It covers all major HESI A2 Biology content areas including
biomolecules, cellular structure, cell cycle, DNA and protein synthesis, genetics,
metabolism, body systems, and ecology. Perfect for nursing school entrance exam
review, this set is aligned with the latest 2026/2027 HESI A2 test blueprint.
Keywords
HESI A2 Biology, HESI A2 exam, HESI A2 practice questions, HESI A2 review,
biology MCQs, nursing entrance exam, biomolecules, cell structure, DNA, protein
synthesis, genetics, body systems, metabolism, HESI 2026, HESI 2027, HESI A2
study guide, HESI A2 Version 2
SECTION 1: BIOMOLECULES AND BASIC CHEMISTRY
(Questions 1–25)
,1. Which of the following is the primary function of carbohydrates in the human
body?
A. Long-term energy storage
B. Immediate energy source
C. Enzyme catalysis
D. Genetic information storage
Correct Answer: B ✅
Rationale: Carbohydrates, especially glucose, are the body's preferred immediate
energy source. They are broken down quickly during cellular respiration. Lipids are used
for long-term energy storage, proteins function as enzymes, and nucleic acids store
genetic information.
2. Which biomolecule is composed of amino acids linked by peptide bonds?
A. Carbohydrates
B. Lipids
C. Proteins
D. Nucleic acids
Correct Answer: C ✅
Rationale: Proteins are polymers of amino acids joined by peptide bonds.
Carbohydrates are made of monosaccharides, lipids of glycerol and fatty acids, and
nucleic acids of nucleotides.
,3. What is the monomer of a nucleic acid?
A. Monosaccharide
B. Amino acid
C. Nucleotide
D. Fatty acid
Correct Answer: C ✅
Rationale: Nucleic acids (DNA and RNA) are polymers of nucleotides, each consisting
of a phosphate group, a five-carbon sugar, and a nitrogenous base.
4. Which of the following is NOT a function of lipids?
A. Insulation
B. Long-term energy storage
C. Carrying genetic information
D. Forming cell membranes
Correct Answer: C ✅
Rationale: Carrying genetic information is the function of nucleic acids, not lipids.
Lipids function in insulation, energy storage, and membrane formation (phospholipid
bilayer).
5. Which element is found in all organic compounds?
A. Nitrogen
B. Carbon
, C. Phosphorus
D. Sulfur
Correct Answer: B ✅
Rationale: Carbon is the backbone of all organic compounds due to its ability to form
four covalent bonds.
6. A solution with a pH of 3 is considered:
A. Neutral
B. Basic
C. Acidic
D. Alkaline
Correct Answer: C ✅
Rationale: A pH below 7 is acidic. A pH of 7 is neutral, and above 7 is basic/alkaline.
7. Which of the following is a disaccharide?
A. Glucose
B. Sucrose
C. Starch
D. Glycogen
Correct Answer: B ✅