HESI A2 BIOLOGY PRACTICE TEST COMPREHENSIVE REVIEW
2026/2027 - QUESTIONS AND ANSWERS 100% VERIFIED
COMPLETE VERIFIED ANSWERS - PASS GUARANTEED - A+
GRADED
149 Questions with Answers and Detailed Rationales
100 PERCENT GUARANTEED PASS
INSTANT DOWNLOAD ANSWERS INCLUDED
IMPORTANCE OF THIS DOCUMENT
This comprehensive examination preparation guide has been meticulously developed to help you succeed in the
HESI A2 BIOLOGY PRACTICE TEST COMPREHENSIVE REVIEW 2026/2027 - QUESTIONS AND ANSWERS
100% VERIFIED COMPLETE VERIFIED ANSWERS - PASS GUARANTEED - A+ GRADED. It contains 149
carefully selected questions that reflect the most current exam content and testing strategies. Each question is
accompanied by a correct answer and a detailed rationale that explains the underlying pathophysiology,
pharmacology, or clinical reasoning.
Self-Assessment – Test your knowledge and Exam Preparation – Familiarize yourself with the
identify areas requiring further question format and content
study areas
Concept Reinforcement – Deepen your Confidence Building – Develop test-taking
understanding through strategies and reduce
evidence-based exam anxiety
rationales
Time Management – Practice answering
questions under simulated
exam conditions
Review Summary 149 Questions
Foundations - Application - HESI A2 Biology Comprehensive Review 2026/2027 AND 100 Complete PASS
Guaranteed A Biology Undergraduate Pre-health/pre-nursing HESI A2 Preparation
All answers with rationales
,Table of Contents
Content Area Questions Key Topics
HESI A2 Biology 1-25 Enzyme, Directly, Meiosis, Mutation, Consequence
Comprehensive Review
2026/2027 AND 100 Complete
PASS Guaranteed A Biology
Undergraduate
Pre-health/pre-nursing HESI
A2 Preparation
Frequency 26-50 Enzyme, Expected, Researcher, Explains, Placed
Solution 51-75 Describes, Allele, Hypertonic Solution, Placed, Organism
Describes 76-100 Frequency, Recessive, Membrane, Individuals, Generation
Heterozygous 101-125 Directly, Hypertonic Solution, Placed, Describes, Phase
Enzyme 126-149 Heterozygous Individuals, Frequency, Describes, Recessive,
Expected
TOTAL 149 All questions include answers and detailed rationales
,Section A - HESI A2 Biology Comprehensive Review
2026/2027 AND 100 Complete PASS Guaranteed A Biology
Undergraduate Pre-health/pre-nursing HESI A2 Preparation
Q1.
A researcher treats cultured cells with a drug that prevents the dissociation of the 60S and
40S ribosomal subunits after translation termination. Which outcome is most directly
expected?
A. Accumulation of free ribosomal subunits B. Inhibition of new translation initiation due
in the cytoplasm to subunit sequestration
C. Enhanced recycling of elongation factors D. Impaired tRNA charging by
aminoacyl-tRNA synthetases
Correct: B - Inhibition of new translation initiation due to subunit sequestration
Rationale:If the 60S and 40S subunits cannot dissociate after termination, they remain as
80S ribosomes and are unavailable for new initiation, which requires free subunits. This
directly blocks translation initiation. The other options do not follow because subunit
dissociation is not required for elongation factor recycling or tRNA charging.
Q2.
In a diploid organism, a cell undergoes meiosis. If nondisjunction of one homologous pair
occurs during meiosis I, what fraction of resulting gametes will be aneuploid?
A. 0% B. 50%
C. 100% D. 25%
Correct: C - 100%
Rationale:Nondisjunction in meiosis I means homologous chromosomes fail to separate, so
all four resulting gametes will have an abnormal chromosome number: two with an extra
chromosome (n+1) and two missing one (n-1). Thus, 100% of gametes are aneuploid.
Options A, B, and D misrepresent the segregation pattern.
Q3.
Which statement best explains why a cell placed in a hypertonic solution shrinks, whereas
a plant cell in the same solution typically does not lyse?
A. Plant cells have contractile vacuoles that B. The cell wall provides rigid support that
pump out excess water. resists expansion but not shrinkage.
Page 3
, Section A - HESI A2 Biology Comprehensive Review 2026/2027 AND 100 Complete PASS Guaranteed A Biology Undergraduate
Pre-health/pre-nursing HESI A2 Preparation
C. Animal cells lack aquaporins, making D. Plant cells actively transport water out to
them more permeable to water. maintain turgor pressure.
Correct: B - The cell wall provides rigid support that resists expansion but not shrinkage.
Rationale:In a hypertonic solution, water leaves both cell types, causing shrinkage. The plant
cell's rigid cell wall prevents bursting (lysis) under hypotonic conditions, but it does not
prevent water loss. The other options misstate mechanisms: contractile vacuoles are not
present in most plant cells, aquaporins are present in animal cells, and water movement is
passive, not active.
Q4.
A mutation in the gene encoding the enzyme phosphofructokinase-1 (PFK-1) reduces its
affinity for fructose-6-phosphate. Which metabolic consequence is most likely?
A. Increased flux through the pentose B. Impaired glycolysis and reduced ATP
phosphate pathway yield from glucose
C. Enhanced gluconeogenesis from lactate D. Accumulation of acetyl-CoA and
increased Krebs cycle activity
Correct: B - Impaired glycolysis and reduced ATP yield from glucose
Rationale:PFK-1 catalyzes the committed step of glycolysis. Reduced affinity for its substrate
slows glycolysis, decreasing ATP production from glucose. The other options are not direct
consequences; the pentose phosphate pathway, gluconeogenesis, and Krebs cycle are not
primarily driven by PFK-1 activity.
Q5.
In the light-dependent reactions of photosynthesis, the proton gradient across the
thylakoid membrane is used to:
A. Directly reduce NADP+ to NADPH B. Drive ATP synthesis via ATP synthase
C. Fix carbon dioxide into glucose D. Split water molecules to release oxygen
Correct: B - Drive ATP synthesis via ATP synthase
Rationale:The proton gradient generated by electron transport across the thylakoid
membrane powers ATP synthase to produce ATP, a process called photophosphorylation.
NADP+ reduction and water splitting occur at photosystem I and II, respectively, and carbon
fixation occurs in the Calvin cycle, not directly from the proton gradient.
Q6.
A DNA template strand has the sequence 3'-TAC GGA TTC-5'. What is the sequence of the
mRNA transcript and the corresponding anticodon on the tRNA that would recognize the
first codon?
Page 4
2026/2027 - QUESTIONS AND ANSWERS 100% VERIFIED
COMPLETE VERIFIED ANSWERS - PASS GUARANTEED - A+
GRADED
149 Questions with Answers and Detailed Rationales
100 PERCENT GUARANTEED PASS
INSTANT DOWNLOAD ANSWERS INCLUDED
IMPORTANCE OF THIS DOCUMENT
This comprehensive examination preparation guide has been meticulously developed to help you succeed in the
HESI A2 BIOLOGY PRACTICE TEST COMPREHENSIVE REVIEW 2026/2027 - QUESTIONS AND ANSWERS
100% VERIFIED COMPLETE VERIFIED ANSWERS - PASS GUARANTEED - A+ GRADED. It contains 149
carefully selected questions that reflect the most current exam content and testing strategies. Each question is
accompanied by a correct answer and a detailed rationale that explains the underlying pathophysiology,
pharmacology, or clinical reasoning.
Self-Assessment – Test your knowledge and Exam Preparation – Familiarize yourself with the
identify areas requiring further question format and content
study areas
Concept Reinforcement – Deepen your Confidence Building – Develop test-taking
understanding through strategies and reduce
evidence-based exam anxiety
rationales
Time Management – Practice answering
questions under simulated
exam conditions
Review Summary 149 Questions
Foundations - Application - HESI A2 Biology Comprehensive Review 2026/2027 AND 100 Complete PASS
Guaranteed A Biology Undergraduate Pre-health/pre-nursing HESI A2 Preparation
All answers with rationales
,Table of Contents
Content Area Questions Key Topics
HESI A2 Biology 1-25 Enzyme, Directly, Meiosis, Mutation, Consequence
Comprehensive Review
2026/2027 AND 100 Complete
PASS Guaranteed A Biology
Undergraduate
Pre-health/pre-nursing HESI
A2 Preparation
Frequency 26-50 Enzyme, Expected, Researcher, Explains, Placed
Solution 51-75 Describes, Allele, Hypertonic Solution, Placed, Organism
Describes 76-100 Frequency, Recessive, Membrane, Individuals, Generation
Heterozygous 101-125 Directly, Hypertonic Solution, Placed, Describes, Phase
Enzyme 126-149 Heterozygous Individuals, Frequency, Describes, Recessive,
Expected
TOTAL 149 All questions include answers and detailed rationales
,Section A - HESI A2 Biology Comprehensive Review
2026/2027 AND 100 Complete PASS Guaranteed A Biology
Undergraduate Pre-health/pre-nursing HESI A2 Preparation
Q1.
A researcher treats cultured cells with a drug that prevents the dissociation of the 60S and
40S ribosomal subunits after translation termination. Which outcome is most directly
expected?
A. Accumulation of free ribosomal subunits B. Inhibition of new translation initiation due
in the cytoplasm to subunit sequestration
C. Enhanced recycling of elongation factors D. Impaired tRNA charging by
aminoacyl-tRNA synthetases
Correct: B - Inhibition of new translation initiation due to subunit sequestration
Rationale:If the 60S and 40S subunits cannot dissociate after termination, they remain as
80S ribosomes and are unavailable for new initiation, which requires free subunits. This
directly blocks translation initiation. The other options do not follow because subunit
dissociation is not required for elongation factor recycling or tRNA charging.
Q2.
In a diploid organism, a cell undergoes meiosis. If nondisjunction of one homologous pair
occurs during meiosis I, what fraction of resulting gametes will be aneuploid?
A. 0% B. 50%
C. 100% D. 25%
Correct: C - 100%
Rationale:Nondisjunction in meiosis I means homologous chromosomes fail to separate, so
all four resulting gametes will have an abnormal chromosome number: two with an extra
chromosome (n+1) and two missing one (n-1). Thus, 100% of gametes are aneuploid.
Options A, B, and D misrepresent the segregation pattern.
Q3.
Which statement best explains why a cell placed in a hypertonic solution shrinks, whereas
a plant cell in the same solution typically does not lyse?
A. Plant cells have contractile vacuoles that B. The cell wall provides rigid support that
pump out excess water. resists expansion but not shrinkage.
Page 3
, Section A - HESI A2 Biology Comprehensive Review 2026/2027 AND 100 Complete PASS Guaranteed A Biology Undergraduate
Pre-health/pre-nursing HESI A2 Preparation
C. Animal cells lack aquaporins, making D. Plant cells actively transport water out to
them more permeable to water. maintain turgor pressure.
Correct: B - The cell wall provides rigid support that resists expansion but not shrinkage.
Rationale:In a hypertonic solution, water leaves both cell types, causing shrinkage. The plant
cell's rigid cell wall prevents bursting (lysis) under hypotonic conditions, but it does not
prevent water loss. The other options misstate mechanisms: contractile vacuoles are not
present in most plant cells, aquaporins are present in animal cells, and water movement is
passive, not active.
Q4.
A mutation in the gene encoding the enzyme phosphofructokinase-1 (PFK-1) reduces its
affinity for fructose-6-phosphate. Which metabolic consequence is most likely?
A. Increased flux through the pentose B. Impaired glycolysis and reduced ATP
phosphate pathway yield from glucose
C. Enhanced gluconeogenesis from lactate D. Accumulation of acetyl-CoA and
increased Krebs cycle activity
Correct: B - Impaired glycolysis and reduced ATP yield from glucose
Rationale:PFK-1 catalyzes the committed step of glycolysis. Reduced affinity for its substrate
slows glycolysis, decreasing ATP production from glucose. The other options are not direct
consequences; the pentose phosphate pathway, gluconeogenesis, and Krebs cycle are not
primarily driven by PFK-1 activity.
Q5.
In the light-dependent reactions of photosynthesis, the proton gradient across the
thylakoid membrane is used to:
A. Directly reduce NADP+ to NADPH B. Drive ATP synthesis via ATP synthase
C. Fix carbon dioxide into glucose D. Split water molecules to release oxygen
Correct: B - Drive ATP synthesis via ATP synthase
Rationale:The proton gradient generated by electron transport across the thylakoid
membrane powers ATP synthase to produce ATP, a process called photophosphorylation.
NADP+ reduction and water splitting occur at photosystem I and II, respectively, and carbon
fixation occurs in the Calvin cycle, not directly from the proton gradient.
Q6.
A DNA template strand has the sequence 3'-TAC GGA TTC-5'. What is the sequence of the
mRNA transcript and the corresponding anticodon on the tRNA that would recognize the
first codon?
Page 4
