WGU Biochemistry OA | Questions with 100% Verified Answers | Latest Update 2026/2027

WGU Biochemistry OA | Questions with 100% Verified
Answers | Latest Update 2026/2027
Question: Bohr Effect chart (low pH)
Answer: C- High CO2
H- High H+
A- Acidic
R-Release of O2(rt. Shift)
T- tense state (in tissues)

Question: Primary structure
Answer: Sequence of amino acids that make up a protein

Question: Secondary Structure
Answer: Sequence of amino acids fold.

Question: Tertiary Structure
Answer: -more folding in a 3D shape.
-R groups (side chains)

Question: Quaternary Structure
Answer: Protein has more than 1 polypeptide chain.

Question: Chemical reaction
Answer: -absorb and release energy
-activation energy
-energy is released (heat & light) when bonds are broken.

Question: Exothermic reaction
Answer: Exo=to release
Thermo=heat
-chemical reaction that releases more energy than it absorbs (as heat & light)

Question: Example of exothermic reaction
Answer: Cellular respiration- breaks down glucose to make cellular energy

Question: Endothermic reaction
Answer: Endo=absorb
Thermos=heat
-absorbs more energy than it releases

Question: Example of endothermic reaction
Answer: Photosynthesis- takes in light to break down to create glucose

,Question: Catalyst
Answer: Substance that will decrease the activation energy needed to start a chemical
reaction

Question: Enzymes
Answer: -catalysts used by cells
-increase the rate of chemical reaction
-specific to substrates

Question: Enzyme structure
Answer: -they are proteins
-twist, fold, bend to final shape
-shape attracts specific molecules

Question: Substrate
Answer: Molecules that bind to the enzyme

Question: Active site
Answer: -location on enzyme where substrate binds

Question: Function of DNA polymerase in process of PCR.
Answer: -increase expression
-2 genes
-SIRT1 & SIRT2

Question: Function of a molecule
Answer: Structural component of the lipid bilayer

Question: How would lipid production in a cell change in order to
maintain fluidity of its cell membrane as it adapts to lower
temperatures?
Answer: Produce lipids with shorter fatty acid chains

Question: Function of cholesterol
Answer: Maintains membrane fluidity

Question: Lipid (fat) soluble vitamins
Answer: A, D, E, K

Question: What stimulates beta-oxidation of fatty acids?
Answer: An increase in NAD+ concentration in the mitochondrial matrix

Question: What occurs in an otherwise healthy person whose diet has
very few carbs and high levels of fats?
Answer: Acetone is produced in the blood

, Question: Amino acids
Answer: -Non-polar (hydrophobic)
-Polar charged
-Polar uncharged

Question: Which type of mammalian DNA damage repair requires the
presence of the chromosome that is homologous to the
damaged chromosome?
Answer: Recombination

Question: Xeroxerma Pigmentosum (XP)
Answer: A recessive genetic disease that occurs when one or more of the genes that perform
nucleotide excision repair are nonfunctional

Question: Why do XP patients have a much higher incidence of skin
cancer than the general population?
Answer: The mutation of all other genes is higher due to failure to repair.

Question: Assuming 100% reaction efficiency, how many DNA copies are
created after the completion of 4 complete PCR cycles?
Answer: 16

Question: What is the function of DNA polymerase in the process of
PCR?
Answer: It recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.

Question: What difference in the regions of the SIRT1 & SIRT2 genes in
people addicted to cocaine increases their expression?
Answer: The nucleosomes become more widely spaced.

Question: What occurs during the process of alternative splicing of
RNA?
Answer: Alternate combinations of Exons within the same gene are linked together.

Question: Polypeptides
Answer: -functional proteins
-made of folded up polymers

Question: Elements
Answer: Substance that cannot be broken down into another substance by chemical reaction.

Question: Ionic bond
Answer: Actions share + & - charge