ASCP CG CERTIFICATION PRACTICE EXAMS
WITH CORRECT ANSWERS
1. What is the best agent to use against mycoplasma? - ANSWER-BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
2. For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWER-comparing the new lot to a lot already in use
3. Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes -
break down tissues into single-cell suspensions
4. When harvesting peripheral blood cultures, asceptic technique no
longer matters following incubation with: - ANSWER-Colcemid - mitotic
spindle poison that halts cells at metaphase
5. Samples that arrive at the cytogenetics lab should first be: - ANSWER-
Logged in to the system
6. Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER-T-cell lymphocytes
7. To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ANSWER-
Expiration dates of reagents used
8. Which type of microscope is used when cleaning a CVS (chorionic
villus) sample? - ANSWER-Dissection
,9. Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ANSWER-Decidua (maternal tissue)
10. When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause?
- ANSWER-innappropriate slide drying time
11. What will help prevent microbial contamination and cross
contamination of cultures? - ANSWER-Asceptic technique
12. When there are not enough mets to complete the study of a
patient/case, what needs to be done next? - ANSWER-prepare
additional slides from any remaining sample
13. When looking at a dropped slide under a phase scope and mets
are very spread out and sparse, what could help fix the issue? -
ANSWER-Increading the drying time by using warm slides
14. What is true of an inverted microscope? - ANSWER-the objectives
are beneath the stage
15. High-resolution banding is indicated for a possible diagnosis of: -
ANSWER-DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip
indent), tubular nose, wide spread eyes, etc
, 16. When verifying if a specimen is appropriate for cytogenetic
analysis, what is the MOST important data to accompany the
specimen? - ANSWER-Reason for referral
17. Which type of specimen should be requested for a cytogenetic
analysis for turner syndrome? - ANSWER-Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
18. A blood sample can be used to study the cytogenetics of a
malignancy only if: - ANSWER-Dividing leukemic cells are present in
circulation
19. All of the cultures in a CO2 incubator failed, what aspects of the
incubator are important to monitor to assess the source of the
problem? - ANSWER-Temperature,
CO2 level, and
Humidity level
20. The most important factor affecting the quality of chromosomes
viewed under a phase contrast scope is: - ANSWER-Slide drying after
the cells have been dropped
21. What is the modal number (default number of chromosomes in a
cell) for a Down syndrome translocation carrier? - ANSWER-45
22. What is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion miscarriages? -
ANSWER-Chorionic villi
WITH CORRECT ANSWERS
1. What is the best agent to use against mycoplasma? - ANSWER-BM
cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
2. For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWER-comparing the new lot to a lot already in use
3. Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes -
break down tissues into single-cell suspensions
4. When harvesting peripheral blood cultures, asceptic technique no
longer matters following incubation with: - ANSWER-Colcemid - mitotic
spindle poison that halts cells at metaphase
5. Samples that arrive at the cytogenetics lab should first be: - ANSWER-
Logged in to the system
6. Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER-T-cell lymphocytes
7. To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ANSWER-
Expiration dates of reagents used
8. Which type of microscope is used when cleaning a CVS (chorionic
villus) sample? - ANSWER-Dissection
,9. Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ANSWER-Decidua (maternal tissue)
10. When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely cause?
- ANSWER-innappropriate slide drying time
11. What will help prevent microbial contamination and cross
contamination of cultures? - ANSWER-Asceptic technique
12. When there are not enough mets to complete the study of a
patient/case, what needs to be done next? - ANSWER-prepare
additional slides from any remaining sample
13. When looking at a dropped slide under a phase scope and mets
are very spread out and sparse, what could help fix the issue? -
ANSWER-Increading the drying time by using warm slides
14. What is true of an inverted microscope? - ANSWER-the objectives
are beneath the stage
15. High-resolution banding is indicated for a possible diagnosis of: -
ANSWER-DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip
indent), tubular nose, wide spread eyes, etc
, 16. When verifying if a specimen is appropriate for cytogenetic
analysis, what is the MOST important data to accompany the
specimen? - ANSWER-Reason for referral
17. Which type of specimen should be requested for a cytogenetic
analysis for turner syndrome? - ANSWER-Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
18. A blood sample can be used to study the cytogenetics of a
malignancy only if: - ANSWER-Dividing leukemic cells are present in
circulation
19. All of the cultures in a CO2 incubator failed, what aspects of the
incubator are important to monitor to assess the source of the
problem? - ANSWER-Temperature,
CO2 level, and
Humidity level
20. The most important factor affecting the quality of chromosomes
viewed under a phase contrast scope is: - ANSWER-Slide drying after
the cells have been dropped
21. What is the modal number (default number of chromosomes in a
cell) for a Down syndrome translocation carrier? - ANSWER-45
22. What is the best tissue to use for both a good success rate and
abnormality detection in spontaneous abortion miscarriages? -
ANSWER-Chorionic villi
