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Exam (elaborations)

MICR 3060 MIDTERM QUESTIONS WITH VERIFIED ANSWERS

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MICR 3060 MIDTERM QUESTIONS WITH VERIFIED ANSWERS

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MICR 3060 MIDTERM QUESTIONS WITH VERIFIED ANSWERS




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1 of 70

Term



what is the purpose of the durham tube?



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it is an inverted tube that
it is a pH indicator; turns from red to
captures gas if it is produced
yellow in acidic pH
during fermentation




to see if bacteria produce neutral
to determine if baceria can use
products (butandiol) from
citrate as a sole carbon source
fermentation


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2 of 70

,Term



why is it important to not allow your carb test to incubate longer than
24 hours?



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makes the bacteria harder to identify bubbling when HOOH added




can result in false negative to determine if baceria can use
result citrate as a sole carbon source


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3 of 70

Term



acid fast stain



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to stain mycobacterium and other high heat, chemicals, acids, UV
bacteria with high lipid content in radiation, starvation, dessication,
their cell walls extreme freezing

, M. smegmatis (acid fast) were
use of single basic dye and all
red bacilli. S. aureus (non acid
bacteria stain same color
fast) were blue
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4 of 70

Definition



to determine if baceria can use citrate as a sole carbon source



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what is the purpose of the durham what is the purpose of the
tube? citrate test?




what is the purpose of the Vogues- what is the purpose of the mixed acid
proskauer test? (MR-VP) test? (MR-VP)


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5 of 70

Term



what does SIM stand for?



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, it fixes the organims to the slide and
sulfide indole motility keeps it from washing off during the
stain




makes the image brighter M. tuberculosis and M. laprae


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6 of 70

Definition



becuse it turns yellow if in acidic enviorment, and fermentation of
glucose causes the media to become acidic



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what is the purpose of the mixed acid why is phenol red used in the
test? (MR-VP) carbohydrate test?




why was the acid fast stain what is the purpose of the Vogues-
developed? proskauer test? (MR-VP)


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7 of 70

Term

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