Page 1 of 27
MICROBIOLOGY MIDTERM EXAM COMPLETE REVIEW
WITH VERIFIED QUESTIONS DETAILED RATIONALES
GRADED A+
Define Micro-
Less than 1mm and cannot see with the naked eye
Need a microscope to see
Microbes, microorganisms, germs, bugs
What are the 5 groups of microorganisms studied?
Bacteria--most famous
Virus--2nd
Fungus--3rd
Protozoa--some but few
Algae--not a human pathogen
What generates 1/2 of the O2 that we breathe and would make life impossible without?
Microbes
What is the smallest simplest single-celled organism?
Bacteria
What is not a cell, can't live by itself and needs to invade a cell?
Virus
What are the two different classification of fungus?
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Molds and yeast--micro
Mushrooms--Macro
What is a mostly single-celled organism that is Animal like?
Protozoa
What are parasites?
Different organisms that range from worms, insects and protozoa that need a host to survive
What are the different applied microbiology fields of study
Immunology
Epidemiology--control spread of disease
Food Micro--relationship between bacteria, food and drink
Agriculture Micro--relationship between bacteria and crop
Industrial Micro--microbes to produce vitamins, AA, Enzymes, etc.
Eukaryotic cells are...
more complete
Prokaryotic cells are...
all micro organisms and lack a nucleus
Characteristics of microorganisms
Small size
Unicellular simplicity
High Growth rate
Adaptability
microscope
instrument used for enlargement of small objects
simple microscope
single lens and a few working parts. not strong
compound microscope
2 magnifying lenses, a visible light sours, a condenser which collects light to direct toward the
object
magnification
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capacity of an optical system to enlarge small objects
ocular lens
one we look through. 10X
objective lens
4 different, 4X, 10X, 40X, 100X
What is resoltion?
Capacity of optical systems to distinguish or separate 2 adjacent objects/points from each other.
represents clarity of image
Types of microscope?
Light--visible light, bright field, dark field, phase contrast, diff interference
Ultraviolet--florescence
Electron--scanning, transmission
what type of microscope do we use in the lab?
bright field light microscope
how does a microscope work?
light source is on the bottom. The light travels from the lamp, hits the condenser lents which
collects light and points it toward the object. the light passes through the objective lens and the
real image of the object forms behind the ocular lens. the image we see is the virtual image
after it asses through the ocular lens and is at the total magnification
Highest magnification for all light microscopes?
2,000X and 200 nm resolution
eubacteria
the common bacteria of the 2 types. (-) WITH cell walls, (+) WITH cell walls, and no cell wall
(mycoplasm)
archebacteria
less common type of bacteria. do not produce peptidoglycan and can live in extreme
environments
appendages
MICROBIOLOGY MIDTERM EXAM COMPLETE REVIEW
WITH VERIFIED QUESTIONS DETAILED RATIONALES
GRADED A+
Define Micro-
Less than 1mm and cannot see with the naked eye
Need a microscope to see
Microbes, microorganisms, germs, bugs
What are the 5 groups of microorganisms studied?
Bacteria--most famous
Virus--2nd
Fungus--3rd
Protozoa--some but few
Algae--not a human pathogen
What generates 1/2 of the O2 that we breathe and would make life impossible without?
Microbes
What is the smallest simplest single-celled organism?
Bacteria
What is not a cell, can't live by itself and needs to invade a cell?
Virus
What are the two different classification of fungus?
,Page 2 of 27
Molds and yeast--micro
Mushrooms--Macro
What is a mostly single-celled organism that is Animal like?
Protozoa
What are parasites?
Different organisms that range from worms, insects and protozoa that need a host to survive
What are the different applied microbiology fields of study
Immunology
Epidemiology--control spread of disease
Food Micro--relationship between bacteria, food and drink
Agriculture Micro--relationship between bacteria and crop
Industrial Micro--microbes to produce vitamins, AA, Enzymes, etc.
Eukaryotic cells are...
more complete
Prokaryotic cells are...
all micro organisms and lack a nucleus
Characteristics of microorganisms
Small size
Unicellular simplicity
High Growth rate
Adaptability
microscope
instrument used for enlargement of small objects
simple microscope
single lens and a few working parts. not strong
compound microscope
2 magnifying lenses, a visible light sours, a condenser which collects light to direct toward the
object
magnification
, Page 3 of 27
capacity of an optical system to enlarge small objects
ocular lens
one we look through. 10X
objective lens
4 different, 4X, 10X, 40X, 100X
What is resoltion?
Capacity of optical systems to distinguish or separate 2 adjacent objects/points from each other.
represents clarity of image
Types of microscope?
Light--visible light, bright field, dark field, phase contrast, diff interference
Ultraviolet--florescence
Electron--scanning, transmission
what type of microscope do we use in the lab?
bright field light microscope
how does a microscope work?
light source is on the bottom. The light travels from the lamp, hits the condenser lents which
collects light and points it toward the object. the light passes through the objective lens and the
real image of the object forms behind the ocular lens. the image we see is the virtual image
after it asses through the ocular lens and is at the total magnification
Highest magnification for all light microscopes?
2,000X and 200 nm resolution
eubacteria
the common bacteria of the 2 types. (-) WITH cell walls, (+) WITH cell walls, and no cell wall
(mycoplasm)
archebacteria
less common type of bacteria. do not produce peptidoglycan and can live in extreme
environments
appendages
