Acs Biochemistry Final Paper With Complete Solutions

ACS BIOCHEMISTRY FINAL PAPER WITH
COMPLETE SOLUTIONS

◉ Ramachandran plots.
Answer: Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance


◉ Protein structure of a helix, B sheet, reverse turn.
Answer: a: LEMARKHQ
B: VICYFTW
RT: PSDNG


◉ how many amino acids in alpha turn helix.
Answer: 3.6


◉ Super secondary structure.
Answer: helix turn helix


◉ parallel vs. antiparallel.
Answer: parallel wedges face same way

,antiparallel face opposite


◉ Isoelectric point (pI).
Answer: pH at which a particular molecule carries no net electrical
charge.


◉ Protein Separation Techniques (ALL).
Answer: Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG


◉ Protein Separation Techniques (H).
Answer: Homogenization
disrupt cells, sonicator, centrifuge


◉ Protein Separation Techniques (S).
Answer: Salting out
different proteins precipitate out at different salt concentrations

, ◉ Protein Separation Techniques (A).
Answer: Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the
group


◉ Protein Separation Techniques (I).
Answer: ion exchange
separation based on charge; + proteins stick to - beads


◉ Protein Separation Techniques (SG).
Answer: Size Exclusion/Gel Filtration
separation based on size


◉ Cuts (protein separation techniques).
Answer: cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep
pellet)


◉ Dialysis.
Answer: protein separation based on bad with pores
1. Moles in bag + moles in buffer