Biochem Exam 1 - LA3 2026 With
Questions And Answers Written
Correctly
the ratio of enzyme activity relative to total protein is called - correct answer
<<<<<correct answer with dettailed pass ✔✔specific activity
which of the following is often the first step in protein purification from a homogenate - correct answer
<<<<<correct answer with dettailed pass ✔✔centrifugation
which type of protein purification relies on the attraction of a protein for a particular chemical group -
correct answer <<<<<correct answer with dettailed pass ✔✔affinity chromatography
Proteins can be separated from small molecules and ions by - correct answer <<<<<correct answer
with dettailed pass ✔✔dialysis, gel filtration
___ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric
focusing - correct answer <<<<<correct answer with dettailed pass
✔✔polyacrylamide/bisacrylamide, a small pore
What is the purpose of adding SDS to gel electrophoersis - correct answer
<<<<<correct answer with dettailed pass ✔✔provides a negative charge to proteins, disrupts
almost all non covalent protein interactions, allows proteins to be separated on the basis of
approximate mass
why is an assay necessary for protein purification studies - correct answer <<<<<correct answer with
dettailed pass ✔✔to track the protein of interest in the purification process, to accurately measure
the amount of the desired protein, to identify the purification steps needed for purification of protein of
interest
Questions And Answers Written
Correctly
the ratio of enzyme activity relative to total protein is called - correct answer
<<<<<correct answer with dettailed pass ✔✔specific activity
which of the following is often the first step in protein purification from a homogenate - correct answer
<<<<<correct answer with dettailed pass ✔✔centrifugation
which type of protein purification relies on the attraction of a protein for a particular chemical group -
correct answer <<<<<correct answer with dettailed pass ✔✔affinity chromatography
Proteins can be separated from small molecules and ions by - correct answer <<<<<correct answer
with dettailed pass ✔✔dialysis, gel filtration
___ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric
focusing - correct answer <<<<<correct answer with dettailed pass
✔✔polyacrylamide/bisacrylamide, a small pore
What is the purpose of adding SDS to gel electrophoersis - correct answer
<<<<<correct answer with dettailed pass ✔✔provides a negative charge to proteins, disrupts
almost all non covalent protein interactions, allows proteins to be separated on the basis of
approximate mass
why is an assay necessary for protein purification studies - correct answer <<<<<correct answer with
dettailed pass ✔✔to track the protein of interest in the purification process, to accurately measure
the amount of the desired protein, to identify the purification steps needed for purification of protein of
interest
