BLOOD BANK FINAL EXAM UPDATED ACTUAL QUESTIONS AND CORRECT ANSWERS

BLOOD BANK FINAL EXAM UPDATED ACTUAL
QUESTIONS AND CORRECT ANSWERS

Question:
1. What are the three ways you can do a crossmatch?
Answer:
- Instant spin
- AHG
- Electronic

Question:
2. What is the weak D phenotype?
Answer:
a quantitative reduction in the amount of D antigen on a RBC
---- no antibody made/normal D epitope -----

Question:
3. What is the partial D phenotype?
Answer:
a qualitative change in the expression of the D epitope or missing parts of the D epitope

Question:
4. What happens in the "regulator pathway" that forms a Rh Null phenotype?
Answer:
There is a mutation in the RHAG gene and/or RhAG protein, so even though the RHD and RHCE genes
are normal their proteins do no get expressed




Question:
5. What happens in the "amorph pathway" that forms a Rh Null phenotype?
Answer:
The RHAG gene and Protein are normal and functioning correctly but a mutation in the RHD/RHCE genes
results in their proteins not being expressed

,Question:
6. weak expression of Kell BGS antigens due to point mutations in the KEL gene
Answer:
Kmod

Question:
7. weak expression of Kell BGS dues to the lack of XK protein
Answer:
McLeod phenotype

Question:
8. What null phenotype can be found in Polynesians and Pacific Islanders?
Answer:
JK (a-b-)

Question:
9. What null phenotype is found in the African American population? What evolutionary advantage comes
with it?
Answer:
Fy (a-b-) Resistant to Plasmodium vivax

Question:
10. What is the F antigen?
Answer:
- present when c and e antigen are present (r and R0)
- anti-f is often seen with anti-e and anti-c

Question:
11. What is the G antigen?
Answer:
Present when either D or C are present

Question:
12. What is the clinical significance of G antibodies?

, Answer:
•Patients with anti-G should receive units negative for D and C antigens
•Pregnant women with anti-D and -C should be investigated for anti-G. If it is only anti-G, they are still
candidates for RhIg.

Question:
13. What pre-analytical factors are required for pre-transfusion testing?
Answer:
- Full name, MRN, and DOB should be on tube and easy to read
- Must be able to obtain the time of draw, date of draw, and the initials of the person who drew it
- SAMPLE MUST BE LABELED BEDSIDE

Question:
14. What does AHG bind?
Answer:
The Fc portion of IgG

Question:
15. List characteristic components of a DAT
Answer:
- In-vivo
- Testing patient RBC's
- No enhancement used
- AHG
- Validate negative results with check cells

Question:
16. List characteristic components of a IAT
Answer:
- In-vitro
- Testing patient plasma/serum
- Liss commonly used for enhancement
- IS ’ 37 ’ AHG
- Validate negative results with check cells

Question:
17. What is used to neutralized α-ABO ?
Answer:
Saliva

Question:
18. What is used to neutralize α-Le ?
Answer:
Saliva

Question:
19. What is used to neutralize α-P ?