ASCB MB : EXTRA – PRACTICE EXAM WITH
CORRECT ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST ALREADY
GRADED A+
Play your way to mastery with fun games
Match Blocks Charms NEW
Practice questions for this set
Learn 1 /7 Study with Learn
ΔCt = 3.3
Choose an answer
When comparing 2 samples in a qPCR
The Klenow fragment of E. coli DNA
1 2 Amplification plot, a log difference
polymerase I is used:
can be seen as approximately:
, _____ after digestion of bacterial What method uses a 3' fluorophore
chromosomes with restriction acceptor probe and a 5' catalyst
3 4
enzymes is used to determine donor probe that fluoresce when the
similarity between bacterial isolates. donor and acceptor come close?
Don't know?
Terms in this set (199)
The Klenow fragment of E. coli DNA to label DNA probes by random primer labeling.
polymerase I is used:
What is a solution hybridization gel mobility shift assay
method for the detection of nucleic
acid:protein interactions?
_____ involves mixing a labeled Gel mobility shift assay
oligonucleotide with a protein extract
to see if a specific interaction occurs,
visible with an apparent increase in
molecular size on a gel due to the
protein:nucleic acid interaction.
Which probe is most often used to alpha satellite DNA probe
detect trisomy 21 by interphase FISH?
, _____ detect specific chromosomes Alpha satellite DNA probe
and numbers of the specific
chromosomes.
Which reagent generates a signal molecular beacon
during the annealing stage of a
quantitative real time PCR reaction?
Methylation of cytosine residues is bisulfite sequencing
involved in the alteration of gene
expression in a number of cancers.
Which sequencing technique is best
suited to determine if methylation of
cytosine residues has taken place in
the promoter region of a given gene?
_____ is a modified chain terminator Bisulfite sequencing
method specifically designed to
detect methylated nucleotides.
Which method is best suited to single strand conformational polymorphism (SSCP)
screen for mutations in a gene having
multiple possible mutations?
_____ is a screening test in which the Single strand conformational polymorphism (SSCP)
exact nature of a mutation does not
need to be known in order to detect
the presence of a mutation.
What is the best method to rule out use of upstream and downstream primers that span
the possibility of genomic DNA giving an exon-intron-exon region of the target
a false-positive result in an RT-PCR
reaction? *amplicaons generated from genomic DNA would
be larger than the specific amplicon generated from
a mature, spliced mRNA
A common use for pulsed-field gel epidemiological typing of bacterial strains.
electrophoresis is:
CORRECT ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST ALREADY
GRADED A+
Play your way to mastery with fun games
Match Blocks Charms NEW
Practice questions for this set
Learn 1 /7 Study with Learn
ΔCt = 3.3
Choose an answer
When comparing 2 samples in a qPCR
The Klenow fragment of E. coli DNA
1 2 Amplification plot, a log difference
polymerase I is used:
can be seen as approximately:
, _____ after digestion of bacterial What method uses a 3' fluorophore
chromosomes with restriction acceptor probe and a 5' catalyst
3 4
enzymes is used to determine donor probe that fluoresce when the
similarity between bacterial isolates. donor and acceptor come close?
Don't know?
Terms in this set (199)
The Klenow fragment of E. coli DNA to label DNA probes by random primer labeling.
polymerase I is used:
What is a solution hybridization gel mobility shift assay
method for the detection of nucleic
acid:protein interactions?
_____ involves mixing a labeled Gel mobility shift assay
oligonucleotide with a protein extract
to see if a specific interaction occurs,
visible with an apparent increase in
molecular size on a gel due to the
protein:nucleic acid interaction.
Which probe is most often used to alpha satellite DNA probe
detect trisomy 21 by interphase FISH?
, _____ detect specific chromosomes Alpha satellite DNA probe
and numbers of the specific
chromosomes.
Which reagent generates a signal molecular beacon
during the annealing stage of a
quantitative real time PCR reaction?
Methylation of cytosine residues is bisulfite sequencing
involved in the alteration of gene
expression in a number of cancers.
Which sequencing technique is best
suited to determine if methylation of
cytosine residues has taken place in
the promoter region of a given gene?
_____ is a modified chain terminator Bisulfite sequencing
method specifically designed to
detect methylated nucleotides.
Which method is best suited to single strand conformational polymorphism (SSCP)
screen for mutations in a gene having
multiple possible mutations?
_____ is a screening test in which the Single strand conformational polymorphism (SSCP)
exact nature of a mutation does not
need to be known in order to detect
the presence of a mutation.
What is the best method to rule out use of upstream and downstream primers that span
the possibility of genomic DNA giving an exon-intron-exon region of the target
a false-positive result in an RT-PCR
reaction? *amplicaons generated from genomic DNA would
be larger than the specific amplicon generated from
a mature, spliced mRNA
A common use for pulsed-field gel epidemiological typing of bacterial strains.
electrophoresis is:
