2027 Exams Includes Frequently Tested Questions With
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1. Which of the fllowing describes genetic engineering?
-The alteration of an organism's genetic, or hereditary, material in a laboratory
-The alteration of an organism's genetic, or hereditary, material in nature
-Allowed in the U.S.
-A precursor step in producing recombinant proteins for therapeutic use
-All of these - Correct Answer: All of these
2. Description of Inverted Microscope - Correct Answer: Light Source is
located above the specimen
3. Definition of Bright Field Microscope - Correct Answer: Objectives are
located above the stage
4. Definition of Confocal Microscope - Correct Answer: Uses a laser light to
scan samples that have been dyed
5. Electron Microscope - Correct Answer: Uses electrons to scan samples in
near-vacuum conditions.
,6. Which of the following is an accurate description of gram-positive bacteria
after Gram staining?
-Pink, because gram-positive bacteria have a thin layer of peptidoglycan in the cell
wall.
-Purple, because gram-positive bacteria have a thick layer of peptidoglycan in the
cell wall.
-Purple, because gram-positive bacteria have a thin layer of peptidoglycan in the
cell wall.
-Pink, because gram-positive bacteria have a thick layer of peptidoglycan in the
cell wall. - Correct Answer: Purple, because gram-positive bacteria have a
thick layer of peptidoglycan in the cell wall.
7. When transferring bacteria using aseptic technique, the correct method is to
move the culture tube over the sterile loop, rather than moving the loop into the
tube, to minimize contamination.
True or False - Correct Answer: True
8. Which of the following should NOT be used to disinfect an area that is
potentially contaminated by DNA?
-Alcohol
-UV Light
-Bleach - Correct Answer: Alcohol
9. A pH meter operates by using the relationship between a solution's pH and the
measured:
,-Viscosity
-Salinity
-Voltage
-Resistivity - Correct Answer: Voltage
10. Why is it necessary to include a "blank" when using a spectrophotometer?
To serve as a positive control
To increase the sensitivity of the spectrophotometer
To dilute the sample
To provide a zero reference - Correct Answer: To provide a zero reference
11. A solution has an absorbance of 0.5 and a concentration of 0.2 M. What
would the absorbance be if the concentration of the solution is doubled?
0.25
2.0
1.0
0.5 - Correct Answer: 1.0
12. Plasmid DNA is purified using anion exchange column chromatography
because DNA is positively charged.
True or False - Correct Answer: False
13. Which of the following should be included in a gel electrophoresis to
determine the sizes of the DNA fragments on the gel?
, DNA Polymerase
Loading dye
Amino Acid sequences
Molecular Weight Markers - Correct Answer: Molecular Weight Markers
14. Gels are used to separate pieces of RNA, DNA, and protein. What type of gel is
typically used for protein electrophoresis?
Polyvinyl acetate
Agar
Polyacrylamide
Agarosamide - Correct Answer: Polyacrylamide
16. If a bacterial cell did not contain restriction enzymes, which of the following
would be likely to occur?
The cell would not be able to replicate DNA.
The cell would create incomplete plasmids.
The cell would be easily infected and lysed by bacteriophages. - Correct
Answer: The cell would be easily infected and lysed by bacteriophages.
17. Two DNA samples with different sequences are cut with the same restriction
enzyme. When they are run on an agarose gel, the resulting bands will match.
True or False - Correct Answer: False