BISC 101 Final Exam Study Questions
with Verified Answers Already Graded
A+ | Brand New Edition
1. Pasteur pipette - ANSWER purpose- to dispense liquids when accuracy is
less important and to gently mix the liquid mixtures by pipetteing liquid in
and out
procedure:
1) attach pipette to rubber bulb
2)draw liquid up slowly
3)dispense liquid into receiving container
2. Eppendorf microcentrifuge - ANSWER purpose: to separate particles within
a mixture into layers according to their relative densities by spinning the
mixture at extremely high speeds -- densest particles found at bottom of tube
procedure:
1)label to falcon tubes
2)add a volume of your sample to the tube and an equal part to to second
tube
3)turn on main power switch
4)press open to centrifuge lid and load tubes MUST BE BALANCED
5)close centrifuge and lock it
6)set speed and timer
7)press start button
3. diffusion - ANSWER Movement of molecules from an area of higher
concentration to an area of lower concentration.
,4. osmosis - ANSWER movement of water through a selectively permeable
membrane from lower to higher concentration
5. You place a cell in a solution that is hypertonic. The membrane is
impermeable to all solutes present. What do you expect will happen in the
first few minutes? - ANSWER Water diffuses out of the cell, causing the
cell to shrivel.
6. Nacl
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER no in water it is
na and cl
7. ethylene glycol
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER yes does not
dissolve
8. distilled water
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER yes does not
dissolve
9. How will you measure RBC membrane permeability in the lab? - ANSWER
By observing the time it takes for the RBCs to lyse in solutions of varying
temperatures.
10.How would a change in temperature increase membrane permeability? -
ANSWER Higher temperatures can disrupt the lipid bilayer, making the
membrane more permeable.
,11.preparing wet mounts on glass slides - ANSWER purpose-to spread a
Sample thin enough to view clearly under microscope
procedure:
1)place a small drop of sample in the middle of glass slide
2)gently place glass cover slip on the side so that the cover slip touches the
sample
3)lower the opposite edge of the cover slip with pencil or Pasteur pipette to
avoid forming bubbles under the cover slip
4)use a tissue to soak up any excess liquid until the cover slip does not move
when the slide is tilted
12.cheesecloth and funnel filter - ANSWER purpose: to filter out larger solids
while allowing liquids and smaller particles to pass through
procedure:
1)label clean test tube rack or set out a clean beaker
2)place funnel on top and place cheese cloth on top of funnel
3)pour the sample slowly into the middle of the cheesecloth
13.spectrometer - ANSWER purpose: to measure how much light of each h
specific wavelength and colour is absorbed by a sample
procedure:
1)transfer sample into new cuvette
2)at spectrometer set up a new file by clicking untitled on top left of screen
then select new experiment, then select absorbance and then vs wavelength
the spectrometer will now warm up
3)when asked, place a cuvette containing a blank solution
4)transfer sample to new cuvette
5) remove blank cuvette, insert sample cuvette
, 6)at top of screen hit collect and then stop
7)on the graphs click the curve of wavelengths of interest
14.compound microscope - ANSWER purpose: to view objects at 40x, 100x,
and 400x magnification
procedure:
1)rotate the objective lenses until the red 4x objective is pointing straight
down then place slide on microscope stage
2)secure using metal spring
3)move slide using stage until it is centered under the 4x lens
4)turn the light intensity adjustment dial down to 5 and turn on microscope
light
5)focus the microscope START WITH STAGE AT TOP AND THEN
LOWER IT SLOWLY
6)without changing focus rotate to 10x lens, use FINE FOCUS knob to
refocus on the object and centre what you wish to see
15.What is the independent variable in RBC lab? - ANSWER Temperature of
the solution
What is the dependent variable in RBC lab? - ANSWER RBC membrane
permeability as indicated by lysis time.
explain why a change in temperature might increase membrane permeability? -
ANSWER A change of temperature effects the permeability of the membrane
because higher temperatures change allow for the phospholipids from the
membrane to move around more freely which increases the cells permeability
which means the higher the temperature, the more that can enter the cell.
with Verified Answers Already Graded
A+ | Brand New Edition
1. Pasteur pipette - ANSWER purpose- to dispense liquids when accuracy is
less important and to gently mix the liquid mixtures by pipetteing liquid in
and out
procedure:
1) attach pipette to rubber bulb
2)draw liquid up slowly
3)dispense liquid into receiving container
2. Eppendorf microcentrifuge - ANSWER purpose: to separate particles within
a mixture into layers according to their relative densities by spinning the
mixture at extremely high speeds -- densest particles found at bottom of tube
procedure:
1)label to falcon tubes
2)add a volume of your sample to the tube and an equal part to to second
tube
3)turn on main power switch
4)press open to centrifuge lid and load tubes MUST BE BALANCED
5)close centrifuge and lock it
6)set speed and timer
7)press start button
3. diffusion - ANSWER Movement of molecules from an area of higher
concentration to an area of lower concentration.
,4. osmosis - ANSWER movement of water through a selectively permeable
membrane from lower to higher concentration
5. You place a cell in a solution that is hypertonic. The membrane is
impermeable to all solutes present. What do you expect will happen in the
first few minutes? - ANSWER Water diffuses out of the cell, causing the
cell to shrivel.
6. Nacl
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER no in water it is
na and cl
7. ethylene glycol
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER yes does not
dissolve
8. distilled water
Based on the chemical properties of each species, do you expect these
species to be able to cross the RBC membrane? - ANSWER yes does not
dissolve
9. How will you measure RBC membrane permeability in the lab? - ANSWER
By observing the time it takes for the RBCs to lyse in solutions of varying
temperatures.
10.How would a change in temperature increase membrane permeability? -
ANSWER Higher temperatures can disrupt the lipid bilayer, making the
membrane more permeable.
,11.preparing wet mounts on glass slides - ANSWER purpose-to spread a
Sample thin enough to view clearly under microscope
procedure:
1)place a small drop of sample in the middle of glass slide
2)gently place glass cover slip on the side so that the cover slip touches the
sample
3)lower the opposite edge of the cover slip with pencil or Pasteur pipette to
avoid forming bubbles under the cover slip
4)use a tissue to soak up any excess liquid until the cover slip does not move
when the slide is tilted
12.cheesecloth and funnel filter - ANSWER purpose: to filter out larger solids
while allowing liquids and smaller particles to pass through
procedure:
1)label clean test tube rack or set out a clean beaker
2)place funnel on top and place cheese cloth on top of funnel
3)pour the sample slowly into the middle of the cheesecloth
13.spectrometer - ANSWER purpose: to measure how much light of each h
specific wavelength and colour is absorbed by a sample
procedure:
1)transfer sample into new cuvette
2)at spectrometer set up a new file by clicking untitled on top left of screen
then select new experiment, then select absorbance and then vs wavelength
the spectrometer will now warm up
3)when asked, place a cuvette containing a blank solution
4)transfer sample to new cuvette
5) remove blank cuvette, insert sample cuvette
, 6)at top of screen hit collect and then stop
7)on the graphs click the curve of wavelengths of interest
14.compound microscope - ANSWER purpose: to view objects at 40x, 100x,
and 400x magnification
procedure:
1)rotate the objective lenses until the red 4x objective is pointing straight
down then place slide on microscope stage
2)secure using metal spring
3)move slide using stage until it is centered under the 4x lens
4)turn the light intensity adjustment dial down to 5 and turn on microscope
light
5)focus the microscope START WITH STAGE AT TOP AND THEN
LOWER IT SLOWLY
6)without changing focus rotate to 10x lens, use FINE FOCUS knob to
refocus on the object and centre what you wish to see
15.What is the independent variable in RBC lab? - ANSWER Temperature of
the solution
What is the dependent variable in RBC lab? - ANSWER RBC membrane
permeability as indicated by lysis time.
explain why a change in temperature might increase membrane permeability? -
ANSWER A change of temperature effects the permeability of the membrane
because higher temperatures change allow for the phospholipids from the
membrane to move around more freely which increases the cells permeability
which means the higher the temperature, the more that can enter the cell.
