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Gain of Function Mutation - CORRECT ANSWER- increased or new protein function
(hypermorphic or neomorphic)
THINK DOMINANT
Loss of Function Mutation - CORRECT ANSWER- No or decreased proetin function (null or
neomorphic)
THINK RECESSIVE
Lac Operon LoF mutations - CORRECT ANSWER- I- (repressor cannot repress)
Z- (B-gal doesn't break down galactose)
Y- (permease doesn't let lactose into cell)
, I^s LoF or GoF? - CORRECT ANSWER- GoF - represses better than wildtype
The lac operon with the repressor is inducible and (negatively/positively) regulated? - CORRECT
ANSWER- negatively, repression must be removed
What will lead to constitutive (continual) transcription of Z, Y, and A? - CORRECT ANSWER-
Repressor cant bind operator, operator cant bind repressor
Merzygote - CORRECT ANSWER- a strain of bacteria that is made diploid by inserting F' factors
(These are small circular pieces of DNA - plasmids that can be taken up by bacteria) .
7 levels of gene regulation expression - CORRECT ANSWER- Regulation of:
1. chromatin remodeling (chromatin to DNA)
2. transcription (to pre-mRNA)
3. splicing and processing (mRNA)
4.transport (nucleus to ribosome)
5. degradation of mRNA (cytoplasm)
6. translational regulation
7. protein modification (product)
Gene X is made at a high level in one cell type and a low level in another cell type. Why? -
CORRECT ANSWER- The two cell types have a different set of transcription factors
(activators/repressors)
If we look at ? gene in different cell types, we will see the exact same DNA- including the
promoter and all its regulatory elements. - CORRECT ANSWER- the same
If we look at ? in one cell, we will see they have different DNA sequences- including the
promoter and all its regulatory elements. - CORRECT ANSWER- two different genes