MICROBIO USF EXAM 2 STUDY GUIDE UPDATED
ACTUAL QUESTIONS AND CORRECT ANSWERS
Question:
1. The viable and total cell counts of bacteria when treated with bacteriostatic antimicrobials are
_____________.
Answer:
The same.
Question:
2. T/F Prokaryotic genomes are typically comprised of a linear DNA chromosome and sometimes a
circular plasmid.
Answer:
False
Question:
3. In bacteria, the RNA polymerase holoenzyme is typically comprised of what?
Answer:
1σ, 2α, β, β' and a ω subunit
Question:
4. For sterilization, autoclaving depends upon a combination of what?
Answer:
High temperature and pressure
Question:
5. T/F Sigma factors guide RNA polymerase to promoters to terminate transcription.
Answer:
False
Question:
6. Why are high levels of resistance to antimicrobials is generally uncommon?
Answer:
Antimicrobials target several different cellular structures
Question:
7. Bacteria condense their DNA into a series of protein-bound domains referred to as __________.
Answer:
The nucleoid
Question:
8. Factor-independent transcriptional termination commonly requires what?
Answer:
Stem-loops
, Question:
9. T/F The process of pasteurization can be used to sterilize liquid food products, such as milk.
Answer:
False
Question:
10. Which of the following introduces supercoiling in DNA?
Answer:
Topo II
Question:
11. Even though the human genome is 1000X the size of the E. coli genome it only has 8X the number of
genes (i.e. 30,000 genes in humans vs. 4,000 genes in E. coli). Why is this?
Answer:
None of the above
Question:
12. T/F A promoter is a non-coding region of DNA that controls the expression of a downstream gene(s).
Answer:
True
Question:
13. Which of the following is NOT a building block of DNA?
Answer:
Ribose
Question:
14. The -35 and -10 sites of a promoter interact with what part of RNA polymerase?
Answer:
The σ subunit
Question:
15. Polymerization of the two strands of DNA during replication___________.
Answer:
All the above
Question:
16. T/F RNA polymerase can initiate transcription in the absence of sigma factor.
Answer:
False
Question:
17. Topoisomerase I ___________.
Answer:
Cuts a single strand of DNA
ACTUAL QUESTIONS AND CORRECT ANSWERS
Question:
1. The viable and total cell counts of bacteria when treated with bacteriostatic antimicrobials are
_____________.
Answer:
The same.
Question:
2. T/F Prokaryotic genomes are typically comprised of a linear DNA chromosome and sometimes a
circular plasmid.
Answer:
False
Question:
3. In bacteria, the RNA polymerase holoenzyme is typically comprised of what?
Answer:
1σ, 2α, β, β' and a ω subunit
Question:
4. For sterilization, autoclaving depends upon a combination of what?
Answer:
High temperature and pressure
Question:
5. T/F Sigma factors guide RNA polymerase to promoters to terminate transcription.
Answer:
False
Question:
6. Why are high levels of resistance to antimicrobials is generally uncommon?
Answer:
Antimicrobials target several different cellular structures
Question:
7. Bacteria condense their DNA into a series of protein-bound domains referred to as __________.
Answer:
The nucleoid
Question:
8. Factor-independent transcriptional termination commonly requires what?
Answer:
Stem-loops
, Question:
9. T/F The process of pasteurization can be used to sterilize liquid food products, such as milk.
Answer:
False
Question:
10. Which of the following introduces supercoiling in DNA?
Answer:
Topo II
Question:
11. Even though the human genome is 1000X the size of the E. coli genome it only has 8X the number of
genes (i.e. 30,000 genes in humans vs. 4,000 genes in E. coli). Why is this?
Answer:
None of the above
Question:
12. T/F A promoter is a non-coding region of DNA that controls the expression of a downstream gene(s).
Answer:
True
Question:
13. Which of the following is NOT a building block of DNA?
Answer:
Ribose
Question:
14. The -35 and -10 sites of a promoter interact with what part of RNA polymerase?
Answer:
The σ subunit
Question:
15. Polymerization of the two strands of DNA during replication___________.
Answer:
All the above
Question:
16. T/F RNA polymerase can initiate transcription in the absence of sigma factor.
Answer:
False
Question:
17. Topoisomerase I ___________.
Answer:
Cuts a single strand of DNA
