MGG EXAM 4 UPDATED ACTUAL QUESTIONS AND CORRECT ANSWERS

MGG EXAM 4 UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS

Question:
1. A FASTA file always begins with a:
a. <
b. @
c. /
d. >
Answer:
D

Question:
2. Besides the > sign, the first line of a FASTA file includes:
a. the read quality data
b. the sequence
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence
Answer:
C

Question:
3. The second line of a FASTA file comprises:
a. the sequence
b. the read quality data
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence
Answer:
A

Question:
4. A FASTQ file always begins with a:
a. <
b. >
c. @
d. /
Answer:
C

Question:
5. The second line of a FASTQ file comprises:
a.the read quality data
b. the accession number for the sequence
c. the sequence
d. the type of sequencing equipment used in obtaining the sequence

, Answer:
C

Question:
6. The fourth line of FASTQ line comprises:
a. the accession number for the sequence
b. the type of sequencing equipment used in obtaining the sequence
c. the read quality data
d. the sequence
Answer:
C

Question:
7. A PHRED score of 40 for a particular base call corresponds to the probability of 1 base call error in how
many base calls? [Note: this is a Review Quiz question; you won't be asked to memorize the formula for
the exam]
a. 40
b. 4,000
c. 10,000
d. 40,000
Answer:
C

Question:
8. What does a PHRED score of 30 or above indicate?
a. Low error rate for the entire read; acceptable quality
b. Low error rate for that base call; acceptable quality
c. High error rate for that base call; unacceptable quality
d. High error rate for the entire read; unacceptable quality
Answer:
B

Question:
9. Which symbol represents a PHRED score of 36 in the Sanger FASTQ system for using ASCII symbols
to represent PHRED scores? [Note: this is a Review Quiz question; you won't be asked to memorize the
table in the exam]
a. $
b. E
c. @
d. m
Answer:
B