MMSC 436 EXAM 1 QUESTIONS WITH VERIFIED
ANSWERS
A physician orders several laboratory tests on a 55-year-old male who is complaining of
generalized (all-over) pain, stiffness, fatigue, and headaches. Based on the following
serum test results, what is the most likely diagnosis: ALP -- significantly increases, GGT
- normal? - Answers - bone disease
Which is the most sensitive enzyme for monitoring chronic alcoholic liver disease? -
Answers - GGT
The recommended assay, because of its specificity and sensitivity for the measurement
of the activity of the CK-MB isoenzyme, is the: - Answers - monoclonal sandwich
immunoassay
The usefulness of analyzing lipoprotein-associated phospholipase A2 for the
identification of individuals at risk for future cardiovascular events is because of its -
Answers - Upregulation in atherosclerotic lesions, particularly complex plaque
Which of the following products are formed from the forward reaction catalyzed by ALT?
- Answers - Pyruvate and glutamate
When a purified enzyme is used as a reagent, such as urease in the measurement of
urea, the enzyme must be ___ concentration so that the reaction follows ___ kinetics -
Answers - Excess, first
The major cellular origin of acid phosphatase activity in serum is: - Answers - prostate
which of the following methods of measuring enzyme activity utilizes an increase in
absorbance at 340 nm as an indicator reaction? - Answers - Forward LD reaction
Which of the following enzymes has multiple molecular forms (isoenzymes) whose
clinical significance is used in diagnosis? - Answers - Lactate dehydrogenase, creatine
kinase, and alkaline phosphatase
One extracellular enzyme whose serum activity is measured clinically is - Answers -
amylase
If you were asked by your laboratory director to set up an enzyme profile (group of
tests) specifically to assess hepatobiliary (biliary obstruction) disease, which one of the
following sets of enzymes would be most appropriate? - Answers - NTP, ATLP, GGT
Activators that are required for normal CK activity include: - Answers - magnesium only
, which of the following enzymes detects very early changes in liver cell damage? -
Answers - GGT
Which of the following enzyme tests is most affected by "hemolysis"? - Answers -
lactate dehydrogenase
heart tissue contains which of the following CK isoenzymes - Answers - MM and MB
Why is heparin the only acceptable anticoagulant to use when obtaining blood samples
for ALP analysis? - Answers - Other anticoagulants contain inhibitors of magnesium
which is a required cofactor for ALP activation
which of the following enzymes requires chloride (Cl-) as an activator? - Answers -
amylase
The enzyme that is inhibited by hemoglobin is - Answers - lipase
Of the following the only clinically analyzed enzyme in the urine is - Answers - amylase
Which one of the following enzymes catalyzes the transfer of an amino group to alpha-
ketoglutarate with the formation of oxaloacetate and glutamate? - Answers - aspartate
aminotransferase
A reaction rate assay measures enzyme ___; an endpoint assay measures enzyme ___
- Answers - activity; activity
Calculate the enzyme activity in IU/L, given the following information
Change in absorbance over 7 minutes = 0.350
Molar absorptivity = 6.22 x 103 L/mol*cm
Light path = 1 cm
Total assay volume = 3.0 mL
Total sample volume = 0.5 mL - Answers - 48 IU/L
enzyme activity calculation - Answers -
which of the following factors that influence enzymatic activity demonstrate able shaped
curve when relating the factor to enzyme activity - Answers - ph and temp
Which type of enzyme inhibitor binds to the active site and is considered to be a
reversible inhibitor? - Answers - competitive
The principal source of serum cholinesterase (CHE) in blood is - Answers - liver
Tartrate-resistant acid phosphatase (TR-ACP) that is present in unhemolyzed serum is
synthesized mainly in - Answers - osteoclasts
ANSWERS
A physician orders several laboratory tests on a 55-year-old male who is complaining of
generalized (all-over) pain, stiffness, fatigue, and headaches. Based on the following
serum test results, what is the most likely diagnosis: ALP -- significantly increases, GGT
- normal? - Answers - bone disease
Which is the most sensitive enzyme for monitoring chronic alcoholic liver disease? -
Answers - GGT
The recommended assay, because of its specificity and sensitivity for the measurement
of the activity of the CK-MB isoenzyme, is the: - Answers - monoclonal sandwich
immunoassay
The usefulness of analyzing lipoprotein-associated phospholipase A2 for the
identification of individuals at risk for future cardiovascular events is because of its -
Answers - Upregulation in atherosclerotic lesions, particularly complex plaque
Which of the following products are formed from the forward reaction catalyzed by ALT?
- Answers - Pyruvate and glutamate
When a purified enzyme is used as a reagent, such as urease in the measurement of
urea, the enzyme must be ___ concentration so that the reaction follows ___ kinetics -
Answers - Excess, first
The major cellular origin of acid phosphatase activity in serum is: - Answers - prostate
which of the following methods of measuring enzyme activity utilizes an increase in
absorbance at 340 nm as an indicator reaction? - Answers - Forward LD reaction
Which of the following enzymes has multiple molecular forms (isoenzymes) whose
clinical significance is used in diagnosis? - Answers - Lactate dehydrogenase, creatine
kinase, and alkaline phosphatase
One extracellular enzyme whose serum activity is measured clinically is - Answers -
amylase
If you were asked by your laboratory director to set up an enzyme profile (group of
tests) specifically to assess hepatobiliary (biliary obstruction) disease, which one of the
following sets of enzymes would be most appropriate? - Answers - NTP, ATLP, GGT
Activators that are required for normal CK activity include: - Answers - magnesium only
, which of the following enzymes detects very early changes in liver cell damage? -
Answers - GGT
Which of the following enzyme tests is most affected by "hemolysis"? - Answers -
lactate dehydrogenase
heart tissue contains which of the following CK isoenzymes - Answers - MM and MB
Why is heparin the only acceptable anticoagulant to use when obtaining blood samples
for ALP analysis? - Answers - Other anticoagulants contain inhibitors of magnesium
which is a required cofactor for ALP activation
which of the following enzymes requires chloride (Cl-) as an activator? - Answers -
amylase
The enzyme that is inhibited by hemoglobin is - Answers - lipase
Of the following the only clinically analyzed enzyme in the urine is - Answers - amylase
Which one of the following enzymes catalyzes the transfer of an amino group to alpha-
ketoglutarate with the formation of oxaloacetate and glutamate? - Answers - aspartate
aminotransferase
A reaction rate assay measures enzyme ___; an endpoint assay measures enzyme ___
- Answers - activity; activity
Calculate the enzyme activity in IU/L, given the following information
Change in absorbance over 7 minutes = 0.350
Molar absorptivity = 6.22 x 103 L/mol*cm
Light path = 1 cm
Total assay volume = 3.0 mL
Total sample volume = 0.5 mL - Answers - 48 IU/L
enzyme activity calculation - Answers -
which of the following factors that influence enzymatic activity demonstrate able shaped
curve when relating the factor to enzyme activity - Answers - ph and temp
Which type of enzyme inhibitor binds to the active site and is considered to be a
reversible inhibitor? - Answers - competitive
The principal source of serum cholinesterase (CHE) in blood is - Answers - liver
Tartrate-resistant acid phosphatase (TR-ACP) that is present in unhemolyzed serum is
synthesized mainly in - Answers - osteoclasts
