BIO 204 FINAL EXAM UPDATED ACTUAL QUESTIONS
AND CORRECT ANSWERS
Question:
1. Lab 1 Learning Objectives
Answer:
-Learn how to apply a SALI to a new piece of lab equipment.
-Describe how to make solutions from either solid chemicals or liquid stock
solutions. Include proper equipment and accurate quantities.
-Demonstrate proper use of micropipettes.
-Demonstrate proper use of the compound microscope.
-List criteria for using the dissecting microscope versus the compound
microscope.
-Define inversion, reversal, field of view, and depth of field.
Question:
2. What does SALI stand for?
Answer:
Strategic Approach to Learning Instrumentation
Question:
3. Three steps of SALI
Answer:
1) Function - what is the tool used for: components
2) Safety - can this tool hurt me? how can i prevent damage to tool and myself:
danger, electrical, breakable
3) Procedure - what are the ordered steps to properly use this tool: set up,training
Question:
4. How would you make 100ml of a 5M stock solution using
the Sodium Chloride above?
NaCl molecular weight: 58.44
Answer:
100ml = 0.1 L
5M=xg/(58.4*0.1) = 29.22g
5M= xg/ (molecular mass*L)
solve for x
Weigh out 29.22g of NaCl and add to beaker of 100m
,Question:
5. Now that you have this 5M stock solution, how would you
dilute it to make 100 ml of a 1M solution?
Answer:
M1V1=M2v2
5M(L)=0.1L*1M
0.02L
Question:
6. How would you use the Sodium Chloride (figure above)
to refill a 500ml IV (intravenous) bag of normal saline
(0.9% solution)?
Answer:
Since it is a 0.9% solution
0.9% = xg/500mL
x= 45g solute
Question:
7. If normal saline is 1X solution, then calculate and describe
how to make a 5X solution? You may select any volume
that you wish.
Answer:
multiply values by 5
29.22g * 5= 146.1g NaCl
then add 146.1g NaCl to 500 mL water
Question:
8. What is the conversion factor for μl to mls? ml to ul?
Place your answers in scientific notation.
Answer:
1 μl is 1 × 10-3 ml
1 ml is 1 × 10+3 μl
ex. 0.0057ml= 5.7ul
2ul = .002ml
Question:
9. Micropipette volume ranges
Answer:
p10 - 0.5-10ul
p100- 11-100ul
p1000- 101-1000ul
, Question:
10. Inversion and reversal
Answer:
image is reflected and inverted by microscope
Question:
11. field of view
Answer:
the diameter of the circle of light seen through a microscope
Question:
12. depth of field
Answer:
vertical range within which the species can be focused
Question:
13. dissecting microscope vs compound microscope
Answer:
- low magnifications and larger objects, surface of specimen
- light underneath
Question:
14. Lab 2 Learning Objectives
Answer:
-Demonstrate the ability to use the proper tool(s) to measure turbidity or
concentration, hydrogen ion concentration, mass, size of an object or organism to
0.01 cm, volume of a liquid, separate mixtures based on density, magnify and view
objects or organisms for structural details or to measure other parameters in BIO
204.
--Demonstrate the ability to make solutions from solid or stock either by % w/v, %
v/v, by parts (ppt, ppm, etc.), molarity, X, or other concentrations normally used in
biology laboratories, or state in writing the procedure for making such a solution.
-Be able to use information from an applied SALI to troubleshoot a tool. For
example, if the Spec absorbance falls below "0," what adjustments might you
make to the spectrophotometer.
Question:
15. how to measure turbidity
Answer:
spectrophotometer
AND CORRECT ANSWERS
Question:
1. Lab 1 Learning Objectives
Answer:
-Learn how to apply a SALI to a new piece of lab equipment.
-Describe how to make solutions from either solid chemicals or liquid stock
solutions. Include proper equipment and accurate quantities.
-Demonstrate proper use of micropipettes.
-Demonstrate proper use of the compound microscope.
-List criteria for using the dissecting microscope versus the compound
microscope.
-Define inversion, reversal, field of view, and depth of field.
Question:
2. What does SALI stand for?
Answer:
Strategic Approach to Learning Instrumentation
Question:
3. Three steps of SALI
Answer:
1) Function - what is the tool used for: components
2) Safety - can this tool hurt me? how can i prevent damage to tool and myself:
danger, electrical, breakable
3) Procedure - what are the ordered steps to properly use this tool: set up,training
Question:
4. How would you make 100ml of a 5M stock solution using
the Sodium Chloride above?
NaCl molecular weight: 58.44
Answer:
100ml = 0.1 L
5M=xg/(58.4*0.1) = 29.22g
5M= xg/ (molecular mass*L)
solve for x
Weigh out 29.22g of NaCl and add to beaker of 100m
,Question:
5. Now that you have this 5M stock solution, how would you
dilute it to make 100 ml of a 1M solution?
Answer:
M1V1=M2v2
5M(L)=0.1L*1M
0.02L
Question:
6. How would you use the Sodium Chloride (figure above)
to refill a 500ml IV (intravenous) bag of normal saline
(0.9% solution)?
Answer:
Since it is a 0.9% solution
0.9% = xg/500mL
x= 45g solute
Question:
7. If normal saline is 1X solution, then calculate and describe
how to make a 5X solution? You may select any volume
that you wish.
Answer:
multiply values by 5
29.22g * 5= 146.1g NaCl
then add 146.1g NaCl to 500 mL water
Question:
8. What is the conversion factor for μl to mls? ml to ul?
Place your answers in scientific notation.
Answer:
1 μl is 1 × 10-3 ml
1 ml is 1 × 10+3 μl
ex. 0.0057ml= 5.7ul
2ul = .002ml
Question:
9. Micropipette volume ranges
Answer:
p10 - 0.5-10ul
p100- 11-100ul
p1000- 101-1000ul
, Question:
10. Inversion and reversal
Answer:
image is reflected and inverted by microscope
Question:
11. field of view
Answer:
the diameter of the circle of light seen through a microscope
Question:
12. depth of field
Answer:
vertical range within which the species can be focused
Question:
13. dissecting microscope vs compound microscope
Answer:
- low magnifications and larger objects, surface of specimen
- light underneath
Question:
14. Lab 2 Learning Objectives
Answer:
-Demonstrate the ability to use the proper tool(s) to measure turbidity or
concentration, hydrogen ion concentration, mass, size of an object or organism to
0.01 cm, volume of a liquid, separate mixtures based on density, magnify and view
objects or organisms for structural details or to measure other parameters in BIO
204.
--Demonstrate the ability to make solutions from solid or stock either by % w/v, %
v/v, by parts (ppt, ppm, etc.), molarity, X, or other concentrations normally used in
biology laboratories, or state in writing the procedure for making such a solution.
-Be able to use information from an applied SALI to troubleshoot a tool. For
example, if the Spec absorbance falls below "0," what adjustments might you
make to the spectrophotometer.
Question:
15. how to measure turbidity
Answer:
spectrophotometer
