PORTAGE LEARNING BIOCHEM MODULE 2
ACTUAL EXAM SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
◉ A particular pathway breaks down proteins into amino acids. Is
this an anabolic or a catabolic pathway?
Answer: Catabolic. This is degradation of a molecule.
◉ The acetyl group is connected to CoA to make Acetyl-CoA. A) What
specific atom of CoA connects to the carbon atom on the acetyl
group? B) What nucleotide base is part of CoA?
Answer: A) Sulfur B) Adenine
◉ Both carbohydrates and lipids are broken down into Acetyl-CoA
for further processing. What pathway does Acetyl-CoA go to next?
Answer: Citric Acid Cycle (CAC)
◉ What is the "ETC" and what happens there?
Answer: Electron transport chain. NADH is converted into ATP at the
ETC.
◉ What is the sign (+ or -) of ΔG for the breakdown of ATP to ADP?
Why is the sign important?
,Answer: (-). A negative means spontaneous, or favorable, and is able
to power other processes.
◉ What molecule (FADH2, ATP, ADP, or CoA) is shown below?
Answer: ADP
◉ What are the two phases of glycolysis? How many reactions in
each?
Answer: The preparatory phase and payoff phase. Five reactions in
each.
◉ How many ATPs are invested in glycolysis?
Answer: 2
◉ What is the net number of ATP gained from glycolysis?
Answer: 2 (2 invested, 4 gained, for a net of 2)
◉ How many reactions of glycolysis have a single arrow "→" in their
reaction equation?
Answer: 3
◉ What is the fate of pyruvate produced in glycolysis?
, Answer: Under anaerobic conditions, it goes to lactate or sometimes
ethanol or other products to regenerate NAD+ for glycolysis. Under
aerobic conditions, it proceeds to the citric acid cycle.
◉ For one molecule of glyceraldehyde-3-phosphate, how many ATP
or NADH are produced (or required) in the glyceraldehyde-3-
phosphate to 1,3-bisphosphoglycerate step in glycolysis?
1 NADH required
1 NADH produced
2 ATP produced
You Answered 1 ATP required
None of the above
Answer: 1 NADH produced
◉ Fill in the appropriate blanks in the following sentence using the
word bank. All words will not be used; words can be used more than
once.
Word (phrase) bank:
Reaction 3
Reaction 6
Reaction 7
ACTUAL EXAM SCRIPT 2026 COMPLETE
QUESTIONS AND SOLUTIONS GRADED A+
◉ A particular pathway breaks down proteins into amino acids. Is
this an anabolic or a catabolic pathway?
Answer: Catabolic. This is degradation of a molecule.
◉ The acetyl group is connected to CoA to make Acetyl-CoA. A) What
specific atom of CoA connects to the carbon atom on the acetyl
group? B) What nucleotide base is part of CoA?
Answer: A) Sulfur B) Adenine
◉ Both carbohydrates and lipids are broken down into Acetyl-CoA
for further processing. What pathway does Acetyl-CoA go to next?
Answer: Citric Acid Cycle (CAC)
◉ What is the "ETC" and what happens there?
Answer: Electron transport chain. NADH is converted into ATP at the
ETC.
◉ What is the sign (+ or -) of ΔG for the breakdown of ATP to ADP?
Why is the sign important?
,Answer: (-). A negative means spontaneous, or favorable, and is able
to power other processes.
◉ What molecule (FADH2, ATP, ADP, or CoA) is shown below?
Answer: ADP
◉ What are the two phases of glycolysis? How many reactions in
each?
Answer: The preparatory phase and payoff phase. Five reactions in
each.
◉ How many ATPs are invested in glycolysis?
Answer: 2
◉ What is the net number of ATP gained from glycolysis?
Answer: 2 (2 invested, 4 gained, for a net of 2)
◉ How many reactions of glycolysis have a single arrow "→" in their
reaction equation?
Answer: 3
◉ What is the fate of pyruvate produced in glycolysis?
, Answer: Under anaerobic conditions, it goes to lactate or sometimes
ethanol or other products to regenerate NAD+ for glycolysis. Under
aerobic conditions, it proceeds to the citric acid cycle.
◉ For one molecule of glyceraldehyde-3-phosphate, how many ATP
or NADH are produced (or required) in the glyceraldehyde-3-
phosphate to 1,3-bisphosphoglycerate step in glycolysis?
1 NADH required
1 NADH produced
2 ATP produced
You Answered 1 ATP required
None of the above
Answer: 1 NADH produced
◉ Fill in the appropriate blanks in the following sentence using the
word bank. All words will not be used; words can be used more than
once.
Word (phrase) bank:
Reaction 3
Reaction 6
Reaction 7
