MOLECULAR DIAGNOSTICS 3RD EDITION
FINAL PAPER SOLVED QUESTIONS WITH
COMPLETE ANSWERS
◉ Responsible for transportation of information - DNA.
Answer: All of the following statements are correctly matched with
their corresponding nucleic acid, EXCEPT:
a. Responsible for amino acid transport - tRNA
b. Location of protein synthesis - rRNA
c. Carries genetic information from the cell nucleus to ribosomes in
the cytoplasm, where it serves as a template for protein synthesis -
mRNA
d. Responsible for transportation of information - DNA
◉ During nucleic acid amplification.
Answer: When are data collected in a real-time PCR procedure?
a. Prior to the addition of fluorescent dyes or probes
b. At a single endpoint
c. During nucleic acid amplification
d. During the cooling stage
,◉ Target amplification and signal amplification.
Answer: What are the two general categories of nucleic acid
amplification (NAA) techniques?
a. Polymerase chain reaction (PCR) and branched-chain DNA (bDNA)
b. Fluorescence in situ hybridization (FISH) and strand displacement
amplification (SDA)
c. Target amplification and signal amplification
d. PCR and reverse transcriptase PCR (RT-PCR)
◉ HSV.
Answer: Which of the following organisms is least likely to be part of
a multiplex PCR respiratory panel?
a. RSV
b. Influenza
c. HSV
d. SARS-CoV-2
◉ A single-stranded polymer that incorporates uracil instead of
thymine.
,Answer: Which of the following definitions describes ribonucleic
acid (RNA)?
a. A double-stranded polymer that houses genetic information
b. A single-stranded polymer that incorporates uracil instead of
thymine
c. All the genes in an organism
d. The genome is organized into discrete elements
◉ Recovery phase of HBV infection.
Answer: The disappearance of HBsAg and HBeAg, the persistence of
Total anti-HBc, the appearance of anti-HBs, and often the presence of
anti-HBe indicates what type of Hepatitis infection?
a. Acute HBV infection
b. Chronic HBV infection
c. Recovery phase of HBV infection
d. Acute HCV infection
◉ The temperature at which DNA denatures into two strands and is
dependent on the nucleotide composition of the molecule.
Answer: Some real-time PCR instruments have the ability to perform
melting curve analysis. What is the melting temperature (Tm) for
primers?
, a. The temperature at which DNA denatures into two strands and is
dependent on the nucleotide composition of the molecule
b. The temperature at which DNA denatures into four strands and is
dependent on the nucleotide composition of the molecule
c. The temperature at which DNA denatures into two strands and is
independent of the nucleotide composition of the molecule
d. The temperature at which RNA denatures into multiple amino
acid strands and is dependent on the nucleotide composition of the
molecule
◉ Nucleic acid testing for HCV.
Answer: Which test is NOT used as a screen for retroviruses in
donated blood?
a. Antibody to HIV type 1 or type 2
b. Nucleic acid testing to detect HIV-1 RNA
c. Antibody to HTLV types I and II
d. Nucleic acid testing for HCV
◉ Giardia duodenalis.
Answer: When parasites are observed in clinical stool specimens,
antigen detection is the preferred method for identification,
providing increased sensitivity over more common microscopy
FINAL PAPER SOLVED QUESTIONS WITH
COMPLETE ANSWERS
◉ Responsible for transportation of information - DNA.
Answer: All of the following statements are correctly matched with
their corresponding nucleic acid, EXCEPT:
a. Responsible for amino acid transport - tRNA
b. Location of protein synthesis - rRNA
c. Carries genetic information from the cell nucleus to ribosomes in
the cytoplasm, where it serves as a template for protein synthesis -
mRNA
d. Responsible for transportation of information - DNA
◉ During nucleic acid amplification.
Answer: When are data collected in a real-time PCR procedure?
a. Prior to the addition of fluorescent dyes or probes
b. At a single endpoint
c. During nucleic acid amplification
d. During the cooling stage
,◉ Target amplification and signal amplification.
Answer: What are the two general categories of nucleic acid
amplification (NAA) techniques?
a. Polymerase chain reaction (PCR) and branched-chain DNA (bDNA)
b. Fluorescence in situ hybridization (FISH) and strand displacement
amplification (SDA)
c. Target amplification and signal amplification
d. PCR and reverse transcriptase PCR (RT-PCR)
◉ HSV.
Answer: Which of the following organisms is least likely to be part of
a multiplex PCR respiratory panel?
a. RSV
b. Influenza
c. HSV
d. SARS-CoV-2
◉ A single-stranded polymer that incorporates uracil instead of
thymine.
,Answer: Which of the following definitions describes ribonucleic
acid (RNA)?
a. A double-stranded polymer that houses genetic information
b. A single-stranded polymer that incorporates uracil instead of
thymine
c. All the genes in an organism
d. The genome is organized into discrete elements
◉ Recovery phase of HBV infection.
Answer: The disappearance of HBsAg and HBeAg, the persistence of
Total anti-HBc, the appearance of anti-HBs, and often the presence of
anti-HBe indicates what type of Hepatitis infection?
a. Acute HBV infection
b. Chronic HBV infection
c. Recovery phase of HBV infection
d. Acute HCV infection
◉ The temperature at which DNA denatures into two strands and is
dependent on the nucleotide composition of the molecule.
Answer: Some real-time PCR instruments have the ability to perform
melting curve analysis. What is the melting temperature (Tm) for
primers?
, a. The temperature at which DNA denatures into two strands and is
dependent on the nucleotide composition of the molecule
b. The temperature at which DNA denatures into four strands and is
dependent on the nucleotide composition of the molecule
c. The temperature at which DNA denatures into two strands and is
independent of the nucleotide composition of the molecule
d. The temperature at which RNA denatures into multiple amino
acid strands and is dependent on the nucleotide composition of the
molecule
◉ Nucleic acid testing for HCV.
Answer: Which test is NOT used as a screen for retroviruses in
donated blood?
a. Antibody to HIV type 1 or type 2
b. Nucleic acid testing to detect HIV-1 RNA
c. Antibody to HTLV types I and II
d. Nucleic acid testing for HCV
◉ Giardia duodenalis.
Answer: When parasites are observed in clinical stool specimens,
antigen detection is the preferred method for identification,
providing increased sensitivity over more common microscopy
