ASCP MLT Exam Study Questions with
Verified Answers Graded A+ 2026
ACD/CPD/CPD2 -Correct Answer ✔-21 days
CPDA-1 -Correct Answer ✔-35 days
Infants
HDFN from ABO -Correct Answer ✔-Spherocytes
1st pregnancy
O mother
Permanent deferral -Correct Answer ✔-Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC -Correct Answer ✔-Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs -Correct Answer ✔-Less than 5 x 10^6 to prevent febrile non
hemolytic reactions
Cryoprecipitate -Correct Answer ✔-Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit -Correct Answer ✔-5000 10000
Irradiated blood -Correct Answer ✔-Prevent GVHD
Ulex europaeus -Correct Answer ✔-Anti H lectin
,Dolichos biflorus -Correct Answer ✔-Anti A1 lectin
Amount of H greatest to least -Correct Answer ✔-O
A2
B
A1
A1B
Most immunogenicity antigen -Correct Answer ✔-D
Weak D -Correct Answer ✔-Negative immediate spin and positive AHG
Weak D donor -Correct Answer ✔-Must be labeled Rh positive
Weak D patient -Correct Answer ✔-Receives D negative
D control -Correct Answer ✔-AB positive
Any D negative
IgM antibodies -Correct Answer ✔-I
H
MN
P1
Lewis
IgG antibodies -Correct Answer ✔-Rh group
K
Duffy
Jk
I, i antibody -Correct Answer ✔-i converts to I as infant matures
Destroyed by enzymes -Correct Answer ✔-MN
S
Duffy
,Enhanced by enzymes -Correct Answer ✔-Rh
Kids
Lewis
I
P1
Dosage -Correct Answer ✔-MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality -Correct Answer ✔-
TRALI, hemolytic transfusion reactions and TACO
IgE antibodies -Correct Answer ✔-Mild Allergic reactions
IgA antibodies -Correct Answer ✔-Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT -Correct Answer ✔-Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
Kernicterus -Correct Answer ✔-Excess bilirubin in newborn
HDFN from Rh -Correct Answer ✔-Retics
DAT positive
Immediate jaundice
After first baby
Rosette -Correct Answer ✔-Screening for fetal hemorrhage
Kleihauer Betke -Correct Answer ✔-Quantitative fetal maternal bleed
, Primary immune response -Correct Answer ✔-IgM
Secondary immune response -Correct Answer ✔-IgG
Type 1 hypersensitivity -Correct Answer ✔-Anaphylactic
Immediate
Type 2 hypersensitivity -Correct Answer ✔-Antibody depending cytotoxicity
Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity -Correct Answer ✔-Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity -Correct Answer ✔-Delayed
Monocytes and lymphocytes
Ouchterlony -Correct Answer ✔-Antibodies added to pre-cut wells in center of
agar plate and patient Sera and standards are alternated in wells surrounding the
center well
EIA/ELISA -Correct Answer ✔-Sandwich technique
HCG
Nephelometry -Correct Answer ✔-Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct -Correct Answer ✔-Add florescence labeled anti-body
to patient tissue wash and examined under fluorescent microscope
Verified Answers Graded A+ 2026
ACD/CPD/CPD2 -Correct Answer ✔-21 days
CPDA-1 -Correct Answer ✔-35 days
Infants
HDFN from ABO -Correct Answer ✔-Spherocytes
1st pregnancy
O mother
Permanent deferral -Correct Answer ✔-Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC -Correct Answer ✔-Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs -Correct Answer ✔-Less than 5 x 10^6 to prevent febrile non
hemolytic reactions
Cryoprecipitate -Correct Answer ✔-Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit -Correct Answer ✔-5000 10000
Irradiated blood -Correct Answer ✔-Prevent GVHD
Ulex europaeus -Correct Answer ✔-Anti H lectin
,Dolichos biflorus -Correct Answer ✔-Anti A1 lectin
Amount of H greatest to least -Correct Answer ✔-O
A2
B
A1
A1B
Most immunogenicity antigen -Correct Answer ✔-D
Weak D -Correct Answer ✔-Negative immediate spin and positive AHG
Weak D donor -Correct Answer ✔-Must be labeled Rh positive
Weak D patient -Correct Answer ✔-Receives D negative
D control -Correct Answer ✔-AB positive
Any D negative
IgM antibodies -Correct Answer ✔-I
H
MN
P1
Lewis
IgG antibodies -Correct Answer ✔-Rh group
K
Duffy
Jk
I, i antibody -Correct Answer ✔-i converts to I as infant matures
Destroyed by enzymes -Correct Answer ✔-MN
S
Duffy
,Enhanced by enzymes -Correct Answer ✔-Rh
Kids
Lewis
I
P1
Dosage -Correct Answer ✔-MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality -Correct Answer ✔-
TRALI, hemolytic transfusion reactions and TACO
IgE antibodies -Correct Answer ✔-Mild Allergic reactions
IgA antibodies -Correct Answer ✔-Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT -Correct Answer ✔-Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
Kernicterus -Correct Answer ✔-Excess bilirubin in newborn
HDFN from Rh -Correct Answer ✔-Retics
DAT positive
Immediate jaundice
After first baby
Rosette -Correct Answer ✔-Screening for fetal hemorrhage
Kleihauer Betke -Correct Answer ✔-Quantitative fetal maternal bleed
, Primary immune response -Correct Answer ✔-IgM
Secondary immune response -Correct Answer ✔-IgG
Type 1 hypersensitivity -Correct Answer ✔-Anaphylactic
Immediate
Type 2 hypersensitivity -Correct Answer ✔-Antibody depending cytotoxicity
Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity -Correct Answer ✔-Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity -Correct Answer ✔-Delayed
Monocytes and lymphocytes
Ouchterlony -Correct Answer ✔-Antibodies added to pre-cut wells in center of
agar plate and patient Sera and standards are alternated in wells surrounding the
center well
EIA/ELISA -Correct Answer ✔-Sandwich technique
HCG
Nephelometry -Correct Answer ✔-Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct -Correct Answer ✔-Add florescence labeled anti-body
to patient tissue wash and examined under fluorescent microscope
