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Due No due date Points 100 Questions 24
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Questions and Answers:
# Question Points Answer
1 a. True or False: Growth media is best 2 a. False (Growth media is designed to simply
suited for distinguishing between two support (and not restrict) microbial growth).
similar species of bacteria.
b. True (Growth media does not contain
b. True or False: Differential media is best restrictive factors, while selective media is best
suited for distinguishing between two used to encourage the growth of one microbe
similar species of bacteria. while simultaneously discouraging the growth
of the other. Since two similar species of
microbes are being studied they must be
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differentiated under similar but just slightly
different conditions (differential media)).
2 a. A researcher is asked to determine 2 a. Differential media
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which of two vials contains E coli and
which contains salmonella. Knowing both b. Selective media
are Gram-negative while only one of Selective media. If additional foreign microbes
them is capable of fermenting lactose, may be present in your sample and you know
which type of media would be best Neisseria meningitides is slow growing, you
on
suited? must restrict the unwanted microbes while
- Growth media supporting the growth of Neisseria
- Differential media meningitides. Thus, you are selectively
- Non-selective and non-differential media encouraging one microbes growth, while
- Selective media selectively restricting others.
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b. A researcher is asked to determine if a
sample contains Neisseria meningitides.
Knowing Neisseria meningitides is slow
growing and other foreign microbes may
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also be present in the culture, which type
of media would be best suited?
- Growth media
- Selective media
- Differential media
- Selective and Differential media
3 a. What are the requirements of a 10 a. A fastidious microbe is an organism with
fastidious microbe? complex growth requirements such that if
absent it will not grow. Enriched medias thus
contain these specific and essential nutrients
required for the growth of a particular subset
of microorganisms.
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Due No due date Points 100 Questions 24
Time Limit 60 Minutes Requires Respondus LockDown Browser
# Question Points Answer
4 a. True or False: LB agar is classified as a 2 a. True (LB agar is the most basic type of agar
non-selective, non-differential media. and like LB media supports the growth of
virtually all microbes without restriction).
b. True or False: LB agar is classified as a
selective, non-differential media. b. False
5 a. The polysaccharide-based hardening 5 a. Seaweed (algae) extract.
agent added to LB media to produce LB
agar is derived from what? b. Agar is used to create a solid, smooth surface
on which microbes can grow.
b. What is agar used for in microbiology?
6 a. Blood agar is which type of medium?
Select all that apply.
- Selective and Differential
2
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a.
Enriched
Differential
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- Enriched (Blood agar can be used to differential between
- Differential species based on its hemolytic activity).
- Selective
7 a. Match the following hemolytic class 5 a.
with its description of activity. 1=B
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2=C
1. Alpha hemolysis 3=A
2. Beta hemolysis
3. Gamma hemolysis
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A. No change.
B. Greenish-brown color
C. Distinct zone of clearing
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8 a. Columbia CNA agar is most closely 2 a. Blood agar (CNA agar is similar to BAP as it
related to which media: is also enriched with blood and allows for
- EMB agar differentiation based on hemolytic patterns).
- MacConkey Agar
- Blood agar b. Gram-Positive (CNA agar restricts Gram-
- Trypticase Soy Agar Negative microbes therefore only Gram-
Positive microbes will grow).
b. Columbia CNA agar is used to isolate:
- Mycobacteria
- Gram-Positive and Gram-Negative
- Gram-Positive
- Gram-Negative
9 a. True or False: Chocolate agar gets its 2 a. False (Chocolate (cocoa) is never added to
brown color from cocoa to produce an the media. The name is derived simply based
enriched media. on the color that actually comes from the