Which immunoassay methodology is the emission of light by molecules
in an excited state with a limited amount of heat as the result of a
chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - Correct Answera. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited
state with a limited amount of heat (luminescence) as the result of a
chemical reaction. Chemiluminescent assays are ultrasensitive and are
widely used in automated immunoassays and DNA probe assay systems.
Chemiluminescence has excellent sensitivity and dynamic range.
Chemiluminescent labels can be attached to an antigen or an antibody.
Most chemiluminescent reagents and conjugates are stable and
relatively nontoxic.
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The inclusion of Auer rods in blast cells can help establish diagnosis of
which type of leukemia?
a.Acute lymphocytic leukemia
b.Acute myelocytic leukemia
c.Chronic lymphocytic leukemia
d.Myelodysplastic syndrome - Correct AnswerCorrect Answer: Acute
myelocytic leukemia
Auer rods can help establish the diagnosis of acute myelocytic leukemia
because these inclusions are not found in acute lymphocytic leukemia.
What instrument suspends cells in fluid flow one at a time through a
focused beam of light?
a.Flow cytometer
b.Gas chromatograph
c.HPLC
d.Mass spectrometer - Correct AnswerCorrect Answer: Flow cytometer
A flow cytometer is an instrument in which cells suspended in fluid flow
one at a time through a focused beam of light. The light is scattered in
patterns characteristic of the cells and their components. A sensor
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detecting the scattered light measures the volume and other
characteristics of individual cells.
Which cellular constituent of blood contains the vital protein
hemoglobin?
a.Erythrocytes
b.Granulocytes
c.Leukocytes
d.Platelets - Correct AnswerCorrect Answer: Erythrocytes
Erythrocytes are nonnucleated biconcave disk-shaped peripheral blood
cells containing hemoglobin. Its primary function is the transport and
delivery of oxygen to tissues.
Which mononuclear phagocytes are important in the defense against
microorganisms, including mycobacteria, fungi, bacteria, protozoa, and
viruses?
a.Electrolytes and monoblasts
b.Lymphocytes and thrombocytes
c.Monocytes and macrophages
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d.Platelets and granulocytes - Correct AnswerCorrect Answer:
Monocytes and macrophages
Monocytes and macrophages play a role in immune response,
phagocytic defense, and the inflammatory response. They secrete
cytokines, remove senescent blood cells, and have antitumor activity.
What is a white blood cell known as in human blood?
a.Erythrocyte
b.Leukocyte
c.Megakaryocyte
d.Thrombocyte - Correct AnswerCorrect Answer: Leukocyte
In human blood, the formed elements are erythrocytes (RBCs),
leukocytes (WBCs), and thrombocytes (platelets).
Which stain helps precipitate residual ribosomal RNA within
reticulocytes?
a.Giemsa stain
b.New methylene blue
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