ALAT COMPREHENSIVE EXAMS QUESTIONS AND
ANSWERS SET A+
✔✔What does PPE stand for? - ✔✔Personal Protective Equipment
✔✔An area is considered a noise hazard at what decible? - ✔✔85 or above
✔✔When protecting against airborne pathogens or allergens, you should wear what
kind of mask? - ✔✔N95 type respirator or a PAPR
✔✔What are 3 key factors for personal protection against radiation? - ✔✔Shielding:
lead aprons
Time: minimize time spent near radioactive materials
Distance: Stay far away from radioactive material
✔✔What kind of injury is most common in the workplace? - ✔✔Ergonomic
✔✔What kind of injuries does ergonomic hazards cause? - ✔✔Musculoskeletal injuries
✔✔What can help avoid repetitive motion injuries? - ✔✔-Alternating tasks to use
different muscle groups
-Take small breaks inbetween standing to put less stress on body
✔✔What are the common allergens people can develope when working with lab
animals? - ✔✔rat/mouse urine and saliva, dander and fur
✔✔What is top priority if an accident results in injury? - ✔✔First Aid
✔✔What does HEPA stand for? - ✔✔high-efficiency partculate air
✔✔How many HEPA filters are on a laminar flow cabinet? - ✔✔2
,✔✔What class of BSCs are used in enclose equipment that has potential of generating
aerosols or dust and for changing small animal cages. What is the con to this BSC? -
✔✔Class 1 BSC, due to pulling unfiltered air it provides no protection to what is inside
the cabinet
✔✔What class of BSCs pulls air into cabinet to filter it and recirculate it.? how many
types of this class of BSC is there? - ✔✔Class 11 BSC. 4 types A1, A2, B1, B2
✔✔What class of BSCs is also known as glove boxes. They have airtight cabinets
designed for high risk biological agents. It uses long heavy rubber gloves attached to
gas-tight ports to reach inside the cabinet. - ✔✔Class 111
✔✔What is another name for animal housing? - ✔✔Primary enclosure
✔✔What kind of caging is meant for rodents? - ✔✔microisolation cages on ventilated
racks
✔✔What kind of cage is meant for larger animals? - ✔✔front-opening cages with solid
bottoms or floor grates
✔✔What cage is used to house nonhuman primates inside? - ✔✔squeeze cages
✔✔Outdoor nonhuman primates and agricultural animals can be housed in what? -
✔✔runs or corrals
✔✔Why is environmental enrichment good? Give 4 reasons. - ✔✔reduce boredom
through mental stimulation, provide sense of security, offer exercise oppertunity, permit
social interaction
✔✔Which regulation mandates that dogs recieve exercise and non human primates be
provided a psychological enrichment program? - ✔✔Animal Welfare and Regulations
Act
✔✔What kind of plastic caging is most common and why? - ✔✔Polysulfone, can
withstand extreme autoclave temperatures
✔✔Which plastic caging is impact and temperature resistant, but is not commonly used
anymore? - ✔✔Polycarbonate
✔✔Which plastic caging is non-durable plastic used for disposable single-use caging
application? - ✔✔Polystyrene
,✔✔Which plastic caging is used for high risk infectious agents and most resistant to
extreme autoclave temperatures and high number of autoclave cycles? -
✔✔Polyphenylsulfone
✔✔Which plastic caging is a single used dispoable caging? - ✔✔Polyethylene (PET)
✔✔Which plastic caging is used for suspended rodent cages, aquatic tanks, and rabbit
cgaes? This material is shatter resistant, but opaque so it is not recommended for
rodent use. - ✔✔Polypropylene
✔✔What is the most common metal used in animal caging? - ✔✔Stainless steel
✔✔Why is aluminum metal not used anymore in animal caging? Why was it liked? -
✔✔Not used due to not being able to stand up to repeated washing and chemical
exposures. It was liked because it is light wieght
✔✔Why is galvanized metal rarely used now in animal caging? What problems did it
have? - ✔✔Problems with rust and corrosion
✔✔Which guideline and regulations defines minimum cage sizes and charcteristics? -
✔✔The Guide and standards of Animal Welfare Regulations
✔✔What kind of cage is used for rodents with a solid bottom and a wire lid on top tightly
closed? - ✔✔Shoebox caging
✔✔What kind of cage is a shoebox cage with a plastic top that has a filter? -
✔✔Microisolation cage
✔✔What kind of microisolation cage is not mechanically ventilated and allows passive
air exchange into and out of the cage? - ✔✔Static microisolation cage
✔✔What is an IVCs ( individually ventillated cage) cage? - ✔✔A microisolation cage
that has a fan on the rack that actively moves air in and out through air flow ports.
✔✔What kind of microisolation cages house specialized animals? - ✔✔biocontainment
cages
✔✔What is a suspended cage? - ✔✔A cage attached to stainless steel runners that
slide forward like a drawer.
✔✔What kind of bottom caging is not recommended for rodents? - ✔✔Wire-bottom
because it can cause foot injuries
, ✔✔What are modular cages? - ✔✔Cages fixed in the rack instead of being suspended.
✔✔For outdoor NHP enclosures what does USDA require? - ✔✔perimter fence at least
6ft high and 3 ft away from any primary enclosures
✔✔Aqautic caging systems must have what? - ✔✔Water source and drain
✔✔Cubicales are designed for what? - ✔✔mini animal rooms within larger room
✔✔What kind of cage monitors feed & water intake along with urine & feces output?
A) Transfer cages
B) Cubicales
C)Metabolism Cages
D) Modular Cages - ✔✔C) Metabolism Cages
✔✔What kind of caging is used for temporary housing and used to move animals within
facilities? - ✔✔Transfer or tranport cages
✔✔Where can you find space recommenations for animal housing? - ✔✔The Guide
✔✔What is microenvironment? - ✔✔Environment conditions within the animals cage
✔✔What is macroenvironment? - ✔✔environment conditions in the room at large
✔✔What temperature should rodent rooms be kept at? - ✔✔72 degrees
✔✔What is the acceptable humidity range in animal rooms? - ✔✔30%-70%
✔✔More air is being pumped into the room than is pumped out. Which pressure is this?
Negaitve or Positive? - ✔✔Positive
✔✔More air is being pumped out of the room than being pumped in. Which pressure is
this? Negative or Positive? - ✔✔Negative
✔✔What does a barrier-maintained animal room protect against? - ✔✔prevents entry of
all infectious agents form getting into a room
✔✔Proper ventilation helps prevent the accumulation of what gases? - ✔✔ammonia
and carbon dioxide
✔✔What are the 3 processes used to kill or remove microorganisms? - ✔✔Sanitization,
disinfection, sterilization
ANSWERS SET A+
✔✔What does PPE stand for? - ✔✔Personal Protective Equipment
✔✔An area is considered a noise hazard at what decible? - ✔✔85 or above
✔✔When protecting against airborne pathogens or allergens, you should wear what
kind of mask? - ✔✔N95 type respirator or a PAPR
✔✔What are 3 key factors for personal protection against radiation? - ✔✔Shielding:
lead aprons
Time: minimize time spent near radioactive materials
Distance: Stay far away from radioactive material
✔✔What kind of injury is most common in the workplace? - ✔✔Ergonomic
✔✔What kind of injuries does ergonomic hazards cause? - ✔✔Musculoskeletal injuries
✔✔What can help avoid repetitive motion injuries? - ✔✔-Alternating tasks to use
different muscle groups
-Take small breaks inbetween standing to put less stress on body
✔✔What are the common allergens people can develope when working with lab
animals? - ✔✔rat/mouse urine and saliva, dander and fur
✔✔What is top priority if an accident results in injury? - ✔✔First Aid
✔✔What does HEPA stand for? - ✔✔high-efficiency partculate air
✔✔How many HEPA filters are on a laminar flow cabinet? - ✔✔2
,✔✔What class of BSCs are used in enclose equipment that has potential of generating
aerosols or dust and for changing small animal cages. What is the con to this BSC? -
✔✔Class 1 BSC, due to pulling unfiltered air it provides no protection to what is inside
the cabinet
✔✔What class of BSCs pulls air into cabinet to filter it and recirculate it.? how many
types of this class of BSC is there? - ✔✔Class 11 BSC. 4 types A1, A2, B1, B2
✔✔What class of BSCs is also known as glove boxes. They have airtight cabinets
designed for high risk biological agents. It uses long heavy rubber gloves attached to
gas-tight ports to reach inside the cabinet. - ✔✔Class 111
✔✔What is another name for animal housing? - ✔✔Primary enclosure
✔✔What kind of caging is meant for rodents? - ✔✔microisolation cages on ventilated
racks
✔✔What kind of cage is meant for larger animals? - ✔✔front-opening cages with solid
bottoms or floor grates
✔✔What cage is used to house nonhuman primates inside? - ✔✔squeeze cages
✔✔Outdoor nonhuman primates and agricultural animals can be housed in what? -
✔✔runs or corrals
✔✔Why is environmental enrichment good? Give 4 reasons. - ✔✔reduce boredom
through mental stimulation, provide sense of security, offer exercise oppertunity, permit
social interaction
✔✔Which regulation mandates that dogs recieve exercise and non human primates be
provided a psychological enrichment program? - ✔✔Animal Welfare and Regulations
Act
✔✔What kind of plastic caging is most common and why? - ✔✔Polysulfone, can
withstand extreme autoclave temperatures
✔✔Which plastic caging is impact and temperature resistant, but is not commonly used
anymore? - ✔✔Polycarbonate
✔✔Which plastic caging is non-durable plastic used for disposable single-use caging
application? - ✔✔Polystyrene
,✔✔Which plastic caging is used for high risk infectious agents and most resistant to
extreme autoclave temperatures and high number of autoclave cycles? -
✔✔Polyphenylsulfone
✔✔Which plastic caging is a single used dispoable caging? - ✔✔Polyethylene (PET)
✔✔Which plastic caging is used for suspended rodent cages, aquatic tanks, and rabbit
cgaes? This material is shatter resistant, but opaque so it is not recommended for
rodent use. - ✔✔Polypropylene
✔✔What is the most common metal used in animal caging? - ✔✔Stainless steel
✔✔Why is aluminum metal not used anymore in animal caging? Why was it liked? -
✔✔Not used due to not being able to stand up to repeated washing and chemical
exposures. It was liked because it is light wieght
✔✔Why is galvanized metal rarely used now in animal caging? What problems did it
have? - ✔✔Problems with rust and corrosion
✔✔Which guideline and regulations defines minimum cage sizes and charcteristics? -
✔✔The Guide and standards of Animal Welfare Regulations
✔✔What kind of cage is used for rodents with a solid bottom and a wire lid on top tightly
closed? - ✔✔Shoebox caging
✔✔What kind of cage is a shoebox cage with a plastic top that has a filter? -
✔✔Microisolation cage
✔✔What kind of microisolation cage is not mechanically ventilated and allows passive
air exchange into and out of the cage? - ✔✔Static microisolation cage
✔✔What is an IVCs ( individually ventillated cage) cage? - ✔✔A microisolation cage
that has a fan on the rack that actively moves air in and out through air flow ports.
✔✔What kind of microisolation cages house specialized animals? - ✔✔biocontainment
cages
✔✔What is a suspended cage? - ✔✔A cage attached to stainless steel runners that
slide forward like a drawer.
✔✔What kind of bottom caging is not recommended for rodents? - ✔✔Wire-bottom
because it can cause foot injuries
, ✔✔What are modular cages? - ✔✔Cages fixed in the rack instead of being suspended.
✔✔For outdoor NHP enclosures what does USDA require? - ✔✔perimter fence at least
6ft high and 3 ft away from any primary enclosures
✔✔Aqautic caging systems must have what? - ✔✔Water source and drain
✔✔Cubicales are designed for what? - ✔✔mini animal rooms within larger room
✔✔What kind of cage monitors feed & water intake along with urine & feces output?
A) Transfer cages
B) Cubicales
C)Metabolism Cages
D) Modular Cages - ✔✔C) Metabolism Cages
✔✔What kind of caging is used for temporary housing and used to move animals within
facilities? - ✔✔Transfer or tranport cages
✔✔Where can you find space recommenations for animal housing? - ✔✔The Guide
✔✔What is microenvironment? - ✔✔Environment conditions within the animals cage
✔✔What is macroenvironment? - ✔✔environment conditions in the room at large
✔✔What temperature should rodent rooms be kept at? - ✔✔72 degrees
✔✔What is the acceptable humidity range in animal rooms? - ✔✔30%-70%
✔✔More air is being pumped into the room than is pumped out. Which pressure is this?
Negaitve or Positive? - ✔✔Positive
✔✔More air is being pumped out of the room than being pumped in. Which pressure is
this? Negative or Positive? - ✔✔Negative
✔✔What does a barrier-maintained animal room protect against? - ✔✔prevents entry of
all infectious agents form getting into a room
✔✔Proper ventilation helps prevent the accumulation of what gases? - ✔✔ammonia
and carbon dioxide
✔✔What are the 3 processes used to kill or remove microorganisms? - ✔✔Sanitization,
disinfection, sterilization
