BIOL 2321: UNIT 3 || Questions and Answers.
The process of transcription and translation is carried out in a test tube. To a test tube is added:
1. from a zebra: transfer RNA's (tRNA), ribosomes
2. from a dragon: messenger RNA (mRNA); RNA polymerase + other necessary enzymes;
ribonucleotides & amino acids.
3. from a dog: DNA
Assume some new protein is synthesized by this cell-free system in the test tube. The proteins of
which animal(s) will be expressed?
Zebra
Dragon
Dragon and Dog
Zebra and Dog
Dog correct answers Dragon and Dog
plasmid correct answers some are transferable to cell
promoter strength correct answers related to the extent amount of the gene expression
sigma factor correct answers in bacteria, it helps RNA polymerase bind to the promoter
regulon correct answers combind of many operon
Lactose-fermenting bacteria metabolize lactose using two proteins, one binds external lactose
and transports it into the cell, the other breaks down the lactose that comes into the cell. The two
proteins are coded for by two genes that are under the control of a single promoter. You carry out
a lactose fermentation test on an unknown bacterium and find that the result is positive. All of the
following among A - E are applicable to this bacterium growing in the lactose broth, EXCEPT:
,A. An operon specific to lactose metabolism is being expressed.
B. The proteins involved in lactose metabolism represent part of the bacterium's transcriptome.
C. The fact that the bacterium possesses the genes for lactose metabolism means that they are
part of its genome.
D. Sigma factor would have been involved in initiating expression of the lactose metabolizing
genes.
E. The lactose metabolism genes would likely have been expressed a correct answers B. The
proteins involved in lactose metabolism represent part of the bacterium's transcriptome.
Transformation:
A. is the process by which a bacterium converts genetic information into a protein
B. may involve the formation and accumulation of competence factors
C. requires cell-to-cell contact
D. is a process initiated by the detection of DNA in the external environment of a bacterium.
E. two of A-D are correct
F. three of A-D are correct correct answers E. two of A-D are correct ( B & D )
Of the choices given below, which would be required to move an F factor from one cell to
another?
A. a sex pilus.
B. competence factors
C. DNA fragments in the environment.
, D. a virus
E. an F- cell
F. two of the above correct answers F. two of the above ( A & E )
Which of the following is in the correct sequence (left to right) of largest to smallest in terms of
the amount of information in a bacterial cell?
1. 1 nucleotide 2. regulon 3. gene 4. genome 5. operon
2, 4, 3, 5, 1
2, 5, 4, 1, 3
4, 2, 3, 5, 1
4, 2, 5, 3, 1
5, 4, 2, 3, 1 correct answers 4, 2, 5, 3,1
genome > regulon > operon > gene > nucleotide
The recipient of Hfr conjugation __________ becomes an F+ cell.
usually
rarely
always
frequently correct answers rarely
Which statement(s) among A-D is/are not applicable to plasmids?
The process of transcription and translation is carried out in a test tube. To a test tube is added:
1. from a zebra: transfer RNA's (tRNA), ribosomes
2. from a dragon: messenger RNA (mRNA); RNA polymerase + other necessary enzymes;
ribonucleotides & amino acids.
3. from a dog: DNA
Assume some new protein is synthesized by this cell-free system in the test tube. The proteins of
which animal(s) will be expressed?
Zebra
Dragon
Dragon and Dog
Zebra and Dog
Dog correct answers Dragon and Dog
plasmid correct answers some are transferable to cell
promoter strength correct answers related to the extent amount of the gene expression
sigma factor correct answers in bacteria, it helps RNA polymerase bind to the promoter
regulon correct answers combind of many operon
Lactose-fermenting bacteria metabolize lactose using two proteins, one binds external lactose
and transports it into the cell, the other breaks down the lactose that comes into the cell. The two
proteins are coded for by two genes that are under the control of a single promoter. You carry out
a lactose fermentation test on an unknown bacterium and find that the result is positive. All of the
following among A - E are applicable to this bacterium growing in the lactose broth, EXCEPT:
,A. An operon specific to lactose metabolism is being expressed.
B. The proteins involved in lactose metabolism represent part of the bacterium's transcriptome.
C. The fact that the bacterium possesses the genes for lactose metabolism means that they are
part of its genome.
D. Sigma factor would have been involved in initiating expression of the lactose metabolizing
genes.
E. The lactose metabolism genes would likely have been expressed a correct answers B. The
proteins involved in lactose metabolism represent part of the bacterium's transcriptome.
Transformation:
A. is the process by which a bacterium converts genetic information into a protein
B. may involve the formation and accumulation of competence factors
C. requires cell-to-cell contact
D. is a process initiated by the detection of DNA in the external environment of a bacterium.
E. two of A-D are correct
F. three of A-D are correct correct answers E. two of A-D are correct ( B & D )
Of the choices given below, which would be required to move an F factor from one cell to
another?
A. a sex pilus.
B. competence factors
C. DNA fragments in the environment.
, D. a virus
E. an F- cell
F. two of the above correct answers F. two of the above ( A & E )
Which of the following is in the correct sequence (left to right) of largest to smallest in terms of
the amount of information in a bacterial cell?
1. 1 nucleotide 2. regulon 3. gene 4. genome 5. operon
2, 4, 3, 5, 1
2, 5, 4, 1, 3
4, 2, 3, 5, 1
4, 2, 5, 3, 1
5, 4, 2, 3, 1 correct answers 4, 2, 5, 3,1
genome > regulon > operon > gene > nucleotide
The recipient of Hfr conjugation __________ becomes an F+ cell.
usually
rarely
always
frequently correct answers rarely
Which statement(s) among A-D is/are not applicable to plasmids?
