BSCI 330 Lab Practical Questions With Correct Answers

BSCI 330 Lab Practical Questions With
Correct Answers

resolution - CORRECT ANSWER✔✔-the ability to distinguish spirit
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objects. The issue with compound light microscope
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Working distance - CORRECT ANSWER✔✔-The distance between the
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bottom of the objective lens and sample at optimal focus
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resolution depends on - CORRECT ANSWER✔✔-1. Numerical aperature
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2. wavelength of light
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NA - CORRECT ANSWER✔✔-measures amount of light the objective lens
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is capable of gathering
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NA = - CORRECT ANSWER✔✔-nsina
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n = refractive index
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a = 1/2 of angle of max cone of light that can enter objective lens
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R - CORRECT ANSWER✔✔-minimal distance objects can be apart in
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order to be resolved as separate
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, R = - CORRECT ANSWER✔✔-lambda/NA
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Lambda - CORRECT ANSWER✔✔-wavelength
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Higher magnification = - CORRECT ANSWER✔✔-longer lens = shorter
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working distance |




Higher resolution = - CORRECT ANSWER✔✔-smaller R
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Bright Field vs Phase Contrast - CORRECT ANSWER✔✔-Phase contrast
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utilized different refractive inde
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How to introduce contrast - CORRECT ANSWER✔✔-1) bright field
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microscopy
2) phase contrast microscopy
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3) fluorescence microscopy
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Fluorescence microscopy - CORRECT ANSWER✔✔-absorb specific
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wavelength (exitation wavelength) and emit another wavelength
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(emission wavelength) |