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Institution: Portage Learning
Course Code: BIOL 322
Course Title: Neuroscience
Exam: Module 3 Exams
Topic: Neuroscience Research Methods
Edition: Latests Version
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, Neuroscience Research Methods
1. T/F: Some Staining Methods Reveal Only A Small Fraction Of The Cells In A
Population, While Other Methods Stain Only Portions Of Cells.
A. True
B. False
ANS. A
Explanation: Different Histological Staining Methods Reveal Different Components Of
Nervous Tissue, And Some Stains Label Only A Subset Or Portion Of Cells.
2. By Using Stains That Reveal _____, The Pathways And Connections Between Cells
Can Be Seen. Either The ____ Itself Or The ______ ____ Surrounding It May Be
Stained.
A. Axons; Axon; Myelin Sheath
B. Nuclei; Nucleus; Cell Membrane
C. Dendrites; Dendrite; Cytoplasm
D. Cell Bodies; Soma; Nucleus
ANS. A.
Explanation: Fiber Staining Can Reveal Axons And Their Pathways. Either The Axon
Itself Or The Myelin Sheath Surrounding It May Be Stained.
3. _____________ Staining Methods Can Be Used To Localize Specific Compounds
Anywhere Is The Nervous System.
A. Nissl
B. Immunocytochemical
C. Myelin
D. Hematoxylin
ANS. B.
,Explanation: Immunocytochemical Staining Uses Antibodies To Localize Specific
Compounds In Nervous Tissue.
4. T/F: A Single Histological Stain Can Reveal All The Features Of Nervous Tissue.
A. True
B. False
ANS. B
Explanation: A Single Stain Is Not Enough To Reveal All Features Of Nervous Tissue.
Several Sections Of The Same Tissue May Need To Be Examined Using Multiple Stains.
5. _____ Stains Reveal Cell Nuclei And Cell Bodies Because These Stains Bind DNA To
RNA In The Nucleus And Nucleolus As Well As To RNA In The Ribosomes Of The
Rough ER; They Don't Stain The Axons And Dendrites Because The Processes Have
Little Or No ER.
A. Golgi
B. Myelin
C. Nissl
D. Fiber
ANS. C.
Explanation: Nissl Stains Bind To Nucleic Acids And Therefore Prominently Reveal
Neuronal Cell Bodies, Nuclei, Nucleoli, And Rough ER-Containing Nissl Substance.
,6. _______ Stains Reveal The Axons And Dendrites Of Nerve Stains. These Stains
Primarily Stain Fibers, But May Also Stain The Cytoskeleton Of Some Cell Bodies.
A. Fiber
B. Nissl
C. Myelin
D. H&E
ANS. A.
Explanation: Fiber Stains Primarily Reveal Neuronal Processes Such As Axons And
Dendrites.
7. _____ Stains Stain The Myelin Sheaths Surrounding Myelinated Axons, And Thus Act
As _____ Stains. These Stains Don't Stain Cell Bodies At All.
A. Nissl; Cell
B. Myelin; Fiber
C. Golgi; Neuronal
D. H&E; Nuclear
ANS. B.
Explanation: Myelin Stains Specifically Reveal Myelin Sheaths Surrounding Myelinated
Axons And Therefore Function As Fiber Stains.
, 8. (Picture) Myelin Stain: Not That In Sections Stained With This Stain, White Matter
(Nerve Fibers) Appears ______, While Gray Matter (Cell Bodies) Appears ________.
This Is The ________ Of Unstained Tissue.
A. Dark; Light; Opposite
B. Light; Dark; Same
C. Blue; Red; Same
D. Red; Blue; Opposite
ANS. A.
Explanation: In A Myelin Stain, Myelinated Fibers Appear Dark While Cell-Body-Rich
Gray Matter Appears Light, Producing The Opposite Appearance From Unstained
Tissue.
9. T/F: The Golgi Technique And Its Variations Are Amongst The Oldest Methods For
Studying Neurons And Glia, Yet They Are Still Widely Used And Are Irreplaceable In
Many Instances; However It's Mechanism Of Action Is Not Understood.
A. True
B. False
ANS. A