BIOC 384: EXAM 1 EBQ, D2Q, AND
PLAYPOSIT QUESTIONS & ANSWERS
Which statement below most accurately describes the key result and primary conclusion
drawn from the cell fusion experiment illustrated in Figure 2.46 in the textbook?
a) Green proteins and pink proteins are present on mouse and human cells,
respectively, but when the two cells are fused together, the resulting heterokaryon is
brown rather than either green or pink.
b) Mouse cells and human cells were allowed to mate to form a zygote consisting of
nuclei from two different species, this result indicated that fusion of cells from two
different mammals is possible in the lab.
c) It was observed that differentially-labeled fluorescent proteins are evenly distributed
in fused heterokaryon cells, indicating that membrane proteins are able to diffuse
laterally in cell membranes.
d) The experiment used viruses to form mouse-human hybrid cells demonstrating that
Sendai virus can be used to create hybrid cells with equal num - ANSWERc) It was
observed that differentially-labeled fluorescent proteins are evenly distributed in fused
heterokaryon cells, indicating that membrane proteins are able to diffuse laterally in cell
membranes.
Put the following hierarchical levels in the correct order representing the structured
organization of life on Earth. The number 1 is the lowest hierarchical level (base of the
pyramid) and the number 7 if the highest hierarchical level (tip of the pyramid).
____phosphoryl group
____mitochondria
____adenine
____cellulose
____manta ray
____ketogenesis
____Great Barrier Reef - ANSWER1, 5, 2, 3, 6, 4, 7
Which three statements below accurately describe the structure and function of nucleic
acids in living cells?
a) DNA is the template for protein synthesis
b) All RNA molecules in cells encode proteins
c) All protein in cells encodes enzymes
d) Viral RNA cannot be the template for DNA synthesis
e) All DNA in a human cell is contained in the nucleus
f) DNA can be the template for DNA synthesis
g) RNA can be the template for protein synthesis
h) DNA can be the template for RNA synthesis - ANSWERf, g, h
,Match each description on the left with the most accurate thermodynamic term on the
right.
____ Thermogenesis in a hibernating bear results from oxidizing fat stores that are
required to keep the bear alive during winter.
____ The difference between the enthalpy (bond energy) and entropy (level or order) of
a reactant and product at a given reaction temperature.
____ A value greater than 1 indicates that the reaction as written is spontaneous in the
forward direction.
____ The ratio of the concentration of products divided by the concentration of reactants
when the rate of product formation is equal to the rate of reactant formation.
____ A positive value indicates that the reaction as written is spontaneous in the
reverse direction.
____ The amount of energy available from the oxidation of 1 gram of glucose is exactly
the same regardless of the physical or biological process required for oxidation to occur.
____ St - ANSWER1, 3, 4, 4, 3, 1, 2, 2
What is the approximate ratio of the base to acid forms of the amino acid glutamate
contained within a protein at pH 7.2 given that the pKa of the carboxyl R group is 4.0.
(Hint: see equation 2.20 on pg. 75). - ANSWERapprox. 1580
Given the energy charge equation below, if a biological system has an EC = 0.8, what is
true about the concentrations of ATP, ADP, and AMP in the system?
a) There is more AMP in the system than ATP and ADP.
b) The concentrations are all equal.
c) There is more ATP in the system than ADP or AMP.
d) There is more ADP in the system than ATP or AMP. - ANSWERc) There is more ATP
in the system than ADP or AMP.
Which two functional groups are involved in producing a peptide bond?
a) alcohol and amino
b) methyl and amino
c) amino and carboxyl
d) amino and thiol - ANSWERc) amino and carboxyl
Select the one TRUE statement below about membranes from the choices below.
a) Membranes form through covalent interactions between phospholipid tail groups.
b) All biological membranes in a cell have the same composition of phospholipids
c) Biological membranes form rigid impermeable barriers around cells.
d) Phospholipids can have both lateral and rotational movement within fluid
membranes. - ANSWERd) Phospholipids can have both lateral and rotational
movement within fluid membranes.
, Energy conversion in living systems is required for what three types of work?
a) osmotic work, chemical work, potential work
b) osmotic work, photosynthetic work, mechanical work
c) osmotic work, chemical work, mechanical work
d) kinetic work, chemical work, mechanical work - ANSWERc) osmotic work, chemical
work, mechanical work
If a cell is placed in a hypotonic solution, then _____________.
a) there will be no net change in flow of water into or out of the cell.
b) solutes in the cell will be pumped out.
c) solutes in the solution will enter the cell.
d) water will flow into the cell.
e) water will flow out of the cell. - ANSWERd) water will flow into the cell.
Identify the four building block biomolecules from the list to the right, and then match
them with the best description of their primary cellular function.
____ cell membranes
____ neurotransmission
____ enzyme catalysis
____ cell recognition
1. nucleotides
2. proteins
3. amino acids
4. glucose
5. pyruvate
6. simple sugars
7. lipids
8. ATP
9. carbohydrates
10. fatty acids
11. acetyl-CoA
12. carbon dioxide - ANSWER10, 3, 1, 6
Which THREE of the following statements about autotrophs and heterotrophs on Earth
are true?
a) Heterotrophs depend on autotrophs for conversion of light energy to chemical
energy.
b) The majority of autotrophs on Earth require aerobic respiration in order to survive.
c) Autotrophs depend on heterotrophs to oxidize sugar and release CO2 into the
atmosphere.
d) Heterotrophs evolved on Earth before autotrophs.
e) Heterotrophs that do not eat autotrophs every day will die in a short time.
PLAYPOSIT QUESTIONS & ANSWERS
Which statement below most accurately describes the key result and primary conclusion
drawn from the cell fusion experiment illustrated in Figure 2.46 in the textbook?
a) Green proteins and pink proteins are present on mouse and human cells,
respectively, but when the two cells are fused together, the resulting heterokaryon is
brown rather than either green or pink.
b) Mouse cells and human cells were allowed to mate to form a zygote consisting of
nuclei from two different species, this result indicated that fusion of cells from two
different mammals is possible in the lab.
c) It was observed that differentially-labeled fluorescent proteins are evenly distributed
in fused heterokaryon cells, indicating that membrane proteins are able to diffuse
laterally in cell membranes.
d) The experiment used viruses to form mouse-human hybrid cells demonstrating that
Sendai virus can be used to create hybrid cells with equal num - ANSWERc) It was
observed that differentially-labeled fluorescent proteins are evenly distributed in fused
heterokaryon cells, indicating that membrane proteins are able to diffuse laterally in cell
membranes.
Put the following hierarchical levels in the correct order representing the structured
organization of life on Earth. The number 1 is the lowest hierarchical level (base of the
pyramid) and the number 7 if the highest hierarchical level (tip of the pyramid).
____phosphoryl group
____mitochondria
____adenine
____cellulose
____manta ray
____ketogenesis
____Great Barrier Reef - ANSWER1, 5, 2, 3, 6, 4, 7
Which three statements below accurately describe the structure and function of nucleic
acids in living cells?
a) DNA is the template for protein synthesis
b) All RNA molecules in cells encode proteins
c) All protein in cells encodes enzymes
d) Viral RNA cannot be the template for DNA synthesis
e) All DNA in a human cell is contained in the nucleus
f) DNA can be the template for DNA synthesis
g) RNA can be the template for protein synthesis
h) DNA can be the template for RNA synthesis - ANSWERf, g, h
,Match each description on the left with the most accurate thermodynamic term on the
right.
____ Thermogenesis in a hibernating bear results from oxidizing fat stores that are
required to keep the bear alive during winter.
____ The difference between the enthalpy (bond energy) and entropy (level or order) of
a reactant and product at a given reaction temperature.
____ A value greater than 1 indicates that the reaction as written is spontaneous in the
forward direction.
____ The ratio of the concentration of products divided by the concentration of reactants
when the rate of product formation is equal to the rate of reactant formation.
____ A positive value indicates that the reaction as written is spontaneous in the
reverse direction.
____ The amount of energy available from the oxidation of 1 gram of glucose is exactly
the same regardless of the physical or biological process required for oxidation to occur.
____ St - ANSWER1, 3, 4, 4, 3, 1, 2, 2
What is the approximate ratio of the base to acid forms of the amino acid glutamate
contained within a protein at pH 7.2 given that the pKa of the carboxyl R group is 4.0.
(Hint: see equation 2.20 on pg. 75). - ANSWERapprox. 1580
Given the energy charge equation below, if a biological system has an EC = 0.8, what is
true about the concentrations of ATP, ADP, and AMP in the system?
a) There is more AMP in the system than ATP and ADP.
b) The concentrations are all equal.
c) There is more ATP in the system than ADP or AMP.
d) There is more ADP in the system than ATP or AMP. - ANSWERc) There is more ATP
in the system than ADP or AMP.
Which two functional groups are involved in producing a peptide bond?
a) alcohol and amino
b) methyl and amino
c) amino and carboxyl
d) amino and thiol - ANSWERc) amino and carboxyl
Select the one TRUE statement below about membranes from the choices below.
a) Membranes form through covalent interactions between phospholipid tail groups.
b) All biological membranes in a cell have the same composition of phospholipids
c) Biological membranes form rigid impermeable barriers around cells.
d) Phospholipids can have both lateral and rotational movement within fluid
membranes. - ANSWERd) Phospholipids can have both lateral and rotational
movement within fluid membranes.
, Energy conversion in living systems is required for what three types of work?
a) osmotic work, chemical work, potential work
b) osmotic work, photosynthetic work, mechanical work
c) osmotic work, chemical work, mechanical work
d) kinetic work, chemical work, mechanical work - ANSWERc) osmotic work, chemical
work, mechanical work
If a cell is placed in a hypotonic solution, then _____________.
a) there will be no net change in flow of water into or out of the cell.
b) solutes in the cell will be pumped out.
c) solutes in the solution will enter the cell.
d) water will flow into the cell.
e) water will flow out of the cell. - ANSWERd) water will flow into the cell.
Identify the four building block biomolecules from the list to the right, and then match
them with the best description of their primary cellular function.
____ cell membranes
____ neurotransmission
____ enzyme catalysis
____ cell recognition
1. nucleotides
2. proteins
3. amino acids
4. glucose
5. pyruvate
6. simple sugars
7. lipids
8. ATP
9. carbohydrates
10. fatty acids
11. acetyl-CoA
12. carbon dioxide - ANSWER10, 3, 1, 6
Which THREE of the following statements about autotrophs and heterotrophs on Earth
are true?
a) Heterotrophs depend on autotrophs for conversion of light energy to chemical
energy.
b) The majority of autotrophs on Earth require aerobic respiration in order to survive.
c) Autotrophs depend on heterotrophs to oxidize sugar and release CO2 into the
atmosphere.
d) Heterotrophs evolved on Earth before autotrophs.
e) Heterotrophs that do not eat autotrophs every day will die in a short time.
