Microbiology Key Concepts | Microbes, Cell Structures, Stains & Culturing Techniques | Exam Prep
A microscopic organism, such as a bacterium, virus, or Microbe
fungus is?
What is the study of fungi called? Mycology
What is the study of protozoa and parasitic worms Parasitology
called?
What branch of microbiology specializes in the study of Virology
viruses?
Which term describes an organism capable of causing Pathogenic
disease?
What is the ecological role of organisms that break down Decomposers
dead organic matter?
Which term refers to the absence of contamination by Asepsis
unwanted organisms?
What is the treatment of disease using chemical Chemotherapy
substances?
Which scientist was the first to observe live Anton van Leeuwenhoek
microorganisms through a microscope?
What major discovery are Watson and Crick credited The double helix structure of DNA
with identifying?
Who disproved the theory of spontaneous generation Louis Pasteur
and developed pasteurization?
Which scientist established a sequence of steps to prove Robert Koch
a specific microbe causes a specific disease?
Who pioneered the use of chemical disinfectants to Joseph Lister
prevent surgical wound infections?
What is the central concept of the Germ Theory of Microorganisms are the cause of infectious diseases.
Disease?
How do Koch's Postulates relate to the Germ Theory of They provide the experimental framework to prove the Germ Theory for a
Disease? specific pathogen.
Which type of microscope produces a dark image Brightfield microscope
against a light background?
, Microbiology Key Concepts | Microbes, Cell Structures, Stains & Culturing Techniques | Exam Prep
Which microscope is best for observing pale objects that Darkfield microscope
are difficult to see in brightfield?
Which microscope utilizes differences in refractive index Phase-contrast microscope
to view living, unstained specimens?
Which type of microscope uses ultraviolet light to excite Fluorescence microscope
specimens?
Which electron microscope is used to view internal ultra- Transmission Electron Microscope (TEM)
structures of thin sections of cells?
Which electron microscope provides detailed three- Scanning Electron Microscope (SEM)
dimensional views of a specimen's surface?
What is the difference between a simple stain and a Simple stains use one dye for shape, while differential stains use multiple dyes to
differential stain? distinguish organisms.
What is the first step (primary stain) of the Gram stain Application of crystal violet
procedure?
What is the second step (mordant) of the Gram stain Application of Gram's iodine
procedure?
What is the third step (decolorizer) of the Gram stain Application of alcohol or acetone
procedure?
What is the fourth step (counterstain) of the Gram stain Application of safranin
procedure?
A laboratory culture containing only a single species of Pure culture
microorganism is called a _____.
What is the purpose of a smear in microbiology? To fix a thin film of microorganisms onto a slide for staining.
Which technique is used to prevent contamination of Aseptic technique
cultures and the environment?
What is the primary purpose of the streak plate method? To isolate individual colonies from a mixed sample.
What complex polysaccharide is used as a solidifying Agar
agent for culture media?
What is the process of introducing microbes into a Inoculation
culture medium called?
A microscopic organism, such as a bacterium, virus, or Microbe
fungus is?
What is the study of fungi called? Mycology
What is the study of protozoa and parasitic worms Parasitology
called?
What branch of microbiology specializes in the study of Virology
viruses?
Which term describes an organism capable of causing Pathogenic
disease?
What is the ecological role of organisms that break down Decomposers
dead organic matter?
Which term refers to the absence of contamination by Asepsis
unwanted organisms?
What is the treatment of disease using chemical Chemotherapy
substances?
Which scientist was the first to observe live Anton van Leeuwenhoek
microorganisms through a microscope?
What major discovery are Watson and Crick credited The double helix structure of DNA
with identifying?
Who disproved the theory of spontaneous generation Louis Pasteur
and developed pasteurization?
Which scientist established a sequence of steps to prove Robert Koch
a specific microbe causes a specific disease?
Who pioneered the use of chemical disinfectants to Joseph Lister
prevent surgical wound infections?
What is the central concept of the Germ Theory of Microorganisms are the cause of infectious diseases.
Disease?
How do Koch's Postulates relate to the Germ Theory of They provide the experimental framework to prove the Germ Theory for a
Disease? specific pathogen.
Which type of microscope produces a dark image Brightfield microscope
against a light background?
, Microbiology Key Concepts | Microbes, Cell Structures, Stains & Culturing Techniques | Exam Prep
Which microscope is best for observing pale objects that Darkfield microscope
are difficult to see in brightfield?
Which microscope utilizes differences in refractive index Phase-contrast microscope
to view living, unstained specimens?
Which type of microscope uses ultraviolet light to excite Fluorescence microscope
specimens?
Which electron microscope is used to view internal ultra- Transmission Electron Microscope (TEM)
structures of thin sections of cells?
Which electron microscope provides detailed three- Scanning Electron Microscope (SEM)
dimensional views of a specimen's surface?
What is the difference between a simple stain and a Simple stains use one dye for shape, while differential stains use multiple dyes to
differential stain? distinguish organisms.
What is the first step (primary stain) of the Gram stain Application of crystal violet
procedure?
What is the second step (mordant) of the Gram stain Application of Gram's iodine
procedure?
What is the third step (decolorizer) of the Gram stain Application of alcohol or acetone
procedure?
What is the fourth step (counterstain) of the Gram stain Application of safranin
procedure?
A laboratory culture containing only a single species of Pure culture
microorganism is called a _____.
What is the purpose of a smear in microbiology? To fix a thin film of microorganisms onto a slide for staining.
Which technique is used to prevent contamination of Aseptic technique
cultures and the environment?
What is the primary purpose of the streak plate method? To isolate individual colonies from a mixed sample.
What complex polysaccharide is used as a solidifying Agar
agent for culture media?
What is the process of introducing microbes into a Inoculation
culture medium called?
