MMBIO 441 EXAM 1 FINAL QUESTIONS
WITH CORRECT ANSWERS
What promoter element is weak in CAP-activated genes compared to
consensus core promoters? - ANSWER -35 box
Developing a drug to block lambda phage. You have reagents to inhibit the
activity of what protein to block the most transcription? - ANSWER N
What about the stages of transcription is true? - ANSWER Localized
melting at promoter happens during initiation
Synthesis of the transcript proceeds in 5' to 3' direction during elongation
What activities are associayed with prokaryotic sigma factor? - ANSWER
regulation of transcription after heat shock
assembly of core pol
binding of core promoter
protein's overall shape is determined by its - ANSWER primary structure
Which of the following is bound by the prokaryotic RNA polymerase
holoenzyme? - ANSWER -10 box
-35 box
UP element
You make mutant strain of E. coli. In presence of lactose and absence of
glucose, no B-galactosidase is made in the mutant. Core promoter is
functioning normally. Arabinose operon functions normally. What is the
mutation? - ANSWER A repressor mutation that prevents binding to
allolactose
"Four cutter" restriction endonuclease cuts every - ANSWER 256 bp
, What is not a type of chromatography? - ANSWER polyacrylamide
WHat DNA has strongest hybridization? - ANSWER A-form DNA:RNA
figure disccussed in calss depicts - ANSWER dna looping with integral and
non-integral number of turns between binding sites
nucleoside vs nucleotide - ANSWER nucleoside doesn't have phosphate
group
nuleotide (phosphate, sugar, and nitrogenous base)
Difference between deoxyadenosine-5'-triphosphate and adenosine-5'-
triphosphate. - ANSWER deoxyadenoisine lacks hydroxy group at 2'
position (H instead)
adenosine - 5- triphosphate has hydroxy group there
Alpha helix and Beta sheet properties - ANSWER Alpha- h bonding
between the same polypeptide.
Beta---H bonds of backbones instead of r groups
H bonds are responsible for formation
Problems with bacterial expression in eukaryotic genes - ANSWER 1.
Bacteria could recognize foreign DNA and destroy it (toxin)
2. forming insoluble protein aggregrates
3. difference in post translational modification
How do you keep a ligated insert in a plasmid from re-ligating? why
unnecessary with directional cloning? - ANSWER you add alkaline
phosphatase that cleaves the 5' phosphate
directional cloning uses two different enzymes so ends don't match and
won't come together
True or False: A protein will see a G-C bind differently if it approaches from
the major groove instead of minor - ANSWER TRUE
WITH CORRECT ANSWERS
What promoter element is weak in CAP-activated genes compared to
consensus core promoters? - ANSWER -35 box
Developing a drug to block lambda phage. You have reagents to inhibit the
activity of what protein to block the most transcription? - ANSWER N
What about the stages of transcription is true? - ANSWER Localized
melting at promoter happens during initiation
Synthesis of the transcript proceeds in 5' to 3' direction during elongation
What activities are associayed with prokaryotic sigma factor? - ANSWER
regulation of transcription after heat shock
assembly of core pol
binding of core promoter
protein's overall shape is determined by its - ANSWER primary structure
Which of the following is bound by the prokaryotic RNA polymerase
holoenzyme? - ANSWER -10 box
-35 box
UP element
You make mutant strain of E. coli. In presence of lactose and absence of
glucose, no B-galactosidase is made in the mutant. Core promoter is
functioning normally. Arabinose operon functions normally. What is the
mutation? - ANSWER A repressor mutation that prevents binding to
allolactose
"Four cutter" restriction endonuclease cuts every - ANSWER 256 bp
, What is not a type of chromatography? - ANSWER polyacrylamide
WHat DNA has strongest hybridization? - ANSWER A-form DNA:RNA
figure disccussed in calss depicts - ANSWER dna looping with integral and
non-integral number of turns between binding sites
nucleoside vs nucleotide - ANSWER nucleoside doesn't have phosphate
group
nuleotide (phosphate, sugar, and nitrogenous base)
Difference between deoxyadenosine-5'-triphosphate and adenosine-5'-
triphosphate. - ANSWER deoxyadenoisine lacks hydroxy group at 2'
position (H instead)
adenosine - 5- triphosphate has hydroxy group there
Alpha helix and Beta sheet properties - ANSWER Alpha- h bonding
between the same polypeptide.
Beta---H bonds of backbones instead of r groups
H bonds are responsible for formation
Problems with bacterial expression in eukaryotic genes - ANSWER 1.
Bacteria could recognize foreign DNA and destroy it (toxin)
2. forming insoluble protein aggregrates
3. difference in post translational modification
How do you keep a ligated insert in a plasmid from re-ligating? why
unnecessary with directional cloning? - ANSWER you add alkaline
phosphatase that cleaves the 5' phosphate
directional cloning uses two different enzymes so ends don't match and
won't come together
True or False: A protein will see a G-C bind differently if it approaches from
the major groove instead of minor - ANSWER TRUE
