BCH 5413 Biochemistry Test Questions
and Answers Verified Solutions Latest
Update 2026/2027
Answer:
Used to study bacterial metabolism and morphology without contamination from other microbes.
●● Growth Medium:
Answer:
Nutrient material used to grow bacteria; must be sterilized before inoculation.
●● Aseptic Technique:
Answer:
Procedures used to prevent contamination of people, materials, the environment, and cultures.
●● Streak Plating:
Answer:
Method used to isolate bacteria by dragging cells across an agar plate to dilute them.
●● Well-Isolated Colonies:
Answer:
Colonies separated enough to likely contain cells from only one bacterium type.
●● Mixed Culture:
Answer:
Culture containing more than one species of microorganism.
●● Colony Morphology:
Answer:
,The visible characteristics of bacterial colonies such as shape, size, color, and texture.
●● Spread Plating:
Answer:
Method where diluted bacteria are spread evenly across agar to isolate or count cells.
●● Serial Dilution:
Answer:
Stepwise dilution process used to reduce bacterial concentration gradually.
●● Confluent Growth (Lawn):
Answer:
Continuous bacterial growth covering the entire agar surface.
●● Direct Microscopic Count:
Answer:
Counting cells directly under a microscope using a counting chamber.
●● Advantage of Direct Microscopic Counts:
Answer:
Rapid way to estimate cell numbers.
●● Disadvantage of Direct Microscopic Counts:
Answer:
Cannot distinguish living cells from dead cells.
●● Counting by Spread Plating:
Answer:
Method of estimating viable bacteria by counting colonies formed on agar plates.
●● Valid Colony Count Range:
Answer:
, Plates with 30-300 colonies are considered accurate for counting.
●● Concentration:
Answer:
Amount of particles or cells per unit volume.
●● Concentration Formula:
Answer:
C = P / V (concentration = particles ÷ volume).
●● Dilution:
Answer:
Reduction in concentration by adding diluent.
●● Dilution Formula:
Answer:
Dilution = volume added ÷ total volume.
●● Diluent:
Answer:
Sterile liquid used to dilute a sample.
●● Microliter (µL):
Answer:
One-thousandth of a milliliter; 1 mL = 1,000 µL.
●● 2-Fold Serial Dilution:
Answer:
Serial dilution where each step is diluted by 1/2.
●● Total Dilution:
Answer:
and Answers Verified Solutions Latest
Update 2026/2027
Answer:
Used to study bacterial metabolism and morphology without contamination from other microbes.
●● Growth Medium:
Answer:
Nutrient material used to grow bacteria; must be sterilized before inoculation.
●● Aseptic Technique:
Answer:
Procedures used to prevent contamination of people, materials, the environment, and cultures.
●● Streak Plating:
Answer:
Method used to isolate bacteria by dragging cells across an agar plate to dilute them.
●● Well-Isolated Colonies:
Answer:
Colonies separated enough to likely contain cells from only one bacterium type.
●● Mixed Culture:
Answer:
Culture containing more than one species of microorganism.
●● Colony Morphology:
Answer:
,The visible characteristics of bacterial colonies such as shape, size, color, and texture.
●● Spread Plating:
Answer:
Method where diluted bacteria are spread evenly across agar to isolate or count cells.
●● Serial Dilution:
Answer:
Stepwise dilution process used to reduce bacterial concentration gradually.
●● Confluent Growth (Lawn):
Answer:
Continuous bacterial growth covering the entire agar surface.
●● Direct Microscopic Count:
Answer:
Counting cells directly under a microscope using a counting chamber.
●● Advantage of Direct Microscopic Counts:
Answer:
Rapid way to estimate cell numbers.
●● Disadvantage of Direct Microscopic Counts:
Answer:
Cannot distinguish living cells from dead cells.
●● Counting by Spread Plating:
Answer:
Method of estimating viable bacteria by counting colonies formed on agar plates.
●● Valid Colony Count Range:
Answer:
, Plates with 30-300 colonies are considered accurate for counting.
●● Concentration:
Answer:
Amount of particles or cells per unit volume.
●● Concentration Formula:
Answer:
C = P / V (concentration = particles ÷ volume).
●● Dilution:
Answer:
Reduction in concentration by adding diluent.
●● Dilution Formula:
Answer:
Dilution = volume added ÷ total volume.
●● Diluent:
Answer:
Sterile liquid used to dilute a sample.
●● Microliter (µL):
Answer:
One-thousandth of a milliliter; 1 mL = 1,000 µL.
●● 2-Fold Serial Dilution:
Answer:
Serial dilution where each step is diluted by 1/2.
●● Total Dilution:
Answer:
