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BMSC 210 -Module 3-Questions And Answers.

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Antisepsis - Answer Removing pathogens from living tissue Acidophiles - Answer grow at extreme acid pH How do bacteria archaea replicate? - Answer binary fission budding form filaments - all must replicate and segregate the genome prior to divison bacterial cell cycle - Answer cell cycle is sequence of events from formation of new cell through the next cell division consists of 3 phases: 1. period of growth after the cell is born chromosome replication and partitioning cytokinesis origin of replication - Answer bacteria have a single origin of replication which is the site at which replication begins terminus - Answer site at which replication is terminated, located opposite of the origin Replisome - Answer group of proteins needed for DNA synthesis DNA replication proceeds in both directions from the origin, this is termed bi-directional replication Septation - Answer formation of cross walls between daughter cells several steps: - selection of site for septum formation - assembly of Z ring (composed of protein FtsZ) - assembly of cell wall synthesizing machinery - constriction of cell and septum formation

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BMSC 210 -Module 3-Questions And
Answers.
Antisepsis - Answer Removing pathogens from living tissue



Acidophiles - Answer grow at extreme acid pH



How do bacteria archaea replicate? - Answer binary fission

budding

form filaments

- all must replicate and segregate the genome prior to divison



bacterial cell cycle - Answer cell cycle is sequence of events from formation of new cell
through the next cell division

consists of 3 phases:

1. period of growth after the cell is born

chromosome replication and partitioning

cytokinesis



origin of replication - Answer bacteria have a single origin of replication which is the site at
which replication begins



terminus - Answer site at which replication is terminated, located opposite of the origin



Replisome - Answer group of proteins needed for DNA synthesis

DNA replication proceeds in both directions from the origin, this is termed bi-directional
replication



Septation - Answer formation of cross walls between daughter cells

several steps:

- selection of site for septum formation

- assembly of Z ring (composed of protein FtsZ)

- assembly of cell wall synthesizing machinery

- constriction of cell and septum formation

, cellular growth is determined by peptidoglycan synthesis in bacteria - Answer - penicillin
binding proteins (PBP's) link peptidoglycan strands and catalyze controlled degradation for new
growth

- Autolysins - PBP enzymes that degrade peptidoglycan at site where new units are added



Cocci divisome - Answer new peptidoglycan forms only at the central septum

- FtsZ determines site of cell wall growth

- Ftsz may recruit PBPs for synthesis of septum



Rods divisome - Answer elongate prior to septation

- MreB determines cell diameter/elongation as Z ring forms



FtsZ - Answer forms Z ring



MreB - Answer helical polymerization throughout cell



Crescentin - Answer intermediate filament homologue

shape-determining protein

localizes to short, curved side of cell (forms curve)



Z ring - Answer structure composed of FtsZ needed for bacterial cell division



Archaeal cell cycle - Answer resembles eukaryal cell cycle

- difference occurs in segregation of chromosomes where they use SegA/SegB protein system



Growth - Answer increase in population



lag phase - Answer "flat" period of adjustment, enlargement; little growth

- adapt to new medium and synthesis new enzymes (maintenance)



exponential phase - Answer -also called log phase

-rate of growth is constant

-population is most uniform in terms of chemical and physical properties during this phase



stationary phase - Answer period of equilibrium; microbial deaths balance production of new
cells

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