Solved Solutions Graded A+.
Which of the following distinguish the field of microbiology from other fields of biology?
A) The techniques used to study organisms regardless of their size.
B) Neither the size of the organism studied nor the techniques employed in the study of
organisms regardless of their size.
C) Both the size of the organism studied and the techniques employed in the study of
organisms.
D)The size of the organism studied. - Answer C
The concept that living organisms arise from nonliving material is called ________.
A) biogenesis
B) germ theory
C) spontaneous generation
D) cell theory - Answer C
In a search for new antibiotics, a previously unknown organism has been recovered from the
soil.It is nonmotile and is composed of long threadlike structures formed from nucleated cells.It
is non-photosynthetic and absorbs its nutrients.This organism will most likely be classified
among the _______.
A) eukaryotes (fungi)
B) bacteria
C) eukaryotes (protozoa)
D) Archaea
E) eukaryotes (algae) - Answer A
Old cultures of bacteria that have lost their ability to cause disease are said to be ________.
A)pathogenic
B) virulent
C) attenuated
D) impotent - Answer C
Antiseptic surgery was pioneered by _______.
A) Kitasato
,B) Jenner
C) Pasteur
D) Lister - Answer D
Three of the SSU rRNA sequences of three organisms have been compared. For organisms 1 and
2, two of the twelve nucleotides in the sequence are different. For organisms 1 and 3, six of the
twelve nucleotides are different. Which organism has greater evolutionary distance from
organism 1?
A) Organism 2
B) The evolutionary distance is the same.
C) Organism 3
D) Evolutionary distance cannot be predicted from this data. - Answer C
Who of the following first discovered that some blood leukocytes could engulf disease-causing
bacteria?
A) Metchnikoff
B) Ivanowski
C) von Behring
D) Meister - Answer A
A student is observing microorganisms in a sample of pond water. One organism of interest has
an obvious nucleus,small oval structures containing a green pigment, and does not appear to be
motile. In which of the following groups would this microbe most likely be classified?
A) Eukaryotes (Algae)
B) Eukaryotes (Fungi)
C) Bacteria
D) Eukaryotes (Protozoa)
E) Archaea - Answer A
A new microbe has been discovered in the rumen of sheep. Microscopy shows no evidence of a
nuclear membrane and biochemical studies of the cell wall demonstrate the lack of
peptidoglycan. Metabolic studies show that this microbe generates methane. This microbe
would most likely be classified in ______.
A) domain Eukarya, Protists
B) domain Archaea
C) domain Eukarya, Kingdom Fungi
D) domain Bacteria - Answer B
,Which group of microbes contains organisms necessary for production of wine and bread?
A) Bacteria
B) Algae
C) Fungi
D) Archaea - Answer C
If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome
would you expect?
A) Gram-negative bacteria would stain purple.
B) Gram-positive bacteria would stain pink.
C) Gram-negative bacteria would be unstained.
D) Gram-negative and Gram-positive bacteria would be unstained. - Answer C
Prior to staining, smears of microorganisms must be heat-fixed in order to __________.
A) attach them firmly to the slide
B) ensure removal of dust particles from the slide surface
C) create small pores in cells that facilitates binding of stain to cell structures
D) allow eventual visualization of internal structures - Answer A
After the primary stain has been added but before the decolorizer has been used, Gram-positive
organisms are stained __________ and Gram-negative organisms are stained __________.
A) purple; pink
B) purple; colorless
C) purple; purple
D) pink; pink - Answer C
Atomic force microscopes use a scanning probe that maintains a fixed distance from the surface
of the specimen. It is useful for specimens that _________.
A) have extremely uneven surfaces
B) neither do not conduct electricity well nor have extremely uneven surfaces is correct
C) both do not conduct electricity well and have extremely uneven surfaces are correct
D) do not conduct electricity well - Answer D
After the mordant has been added, Gram-positive organisms are stained __________ and
Gram-negative organisms are stained __________.
A) purple; colorless
, B) pink; pink
C) purple; purple
D) purple; pink - Answer C
If the objective lenses of a microscope can be changed without losing focus on the specimen,
they are said to be _________.
A) parfocal
B) optifocal
C) equifocal
D) totifocal - Answer A
If the decolorizer is left on too long in the Gram-staining procedure, Gram-positive organisms
will be stained __________ and Gram-negative organisms will be stained __________.
A) purple; colorless
B) purple; blue
C) pink; pink
D) purple; pink - Answer C
If the strength of a lens is the reciprocal of its focal length ( 1/ f ), which of the following lenses
will have the greatest strength?
A) A lens with a focal length of 100 mm
B) A lens with a focal length of 0.1 mm
C) A lens with a focal length of 1cm
D) A lens with a focal length of 1 mm - Answer A
A 45× objective and a 10× ocular produce a total magnification of ________.
A) 900×
B) 55×
C) 145×
D) 450× - Answer D
Which type of microscopy would be preferred for showing fine internal detail of the eukaryotic
organelles?
A) Transmission electron microscopy
B) Bright-field microscopy
C) Scanning electron microscopy