Synthesis-Questions and Answers.
All amino acids have at least one - Answer nitrogen atom
an important question to ask as we begin to look at amino acid synthesis is where does the
nitrogen come from, and how is it incorporated into amino acids?
the enzyme glutamine synthetase plays a central role in - Answer overall nitrogen metabolism
and in the synthesis of many nitrogen-containing biomolecules
Amino acid synthesis obviously require other types of reactions to - Answer incorporate the
carbon and oxygen atoms needed
intermediates of other metabolic pathways are often used to - Answer synthesize amino acids
synthetic pathways of amino acids and their regulation serve as important examples of - Answer
different types of feedback inhibition
While the air we breathe is rich in nitrogen gas (N2) and is the major component in air at 78%,
this nitrogen is unavailable to us since___ - Answer nitrogen gas is very inert chemically
nitrogen assimilation refers to - Answer the formation of organic nitrogen molecules such as
amino acids from inorganic nitrogen compounds in the environment
there are ___ steps involved in nitrogen assimilation - Answer 3
what is involved in step 1 of nitrogen assimilation - Answer the fixation of nitrogen by bacteria
that reside in soil and legume root nodules into usable forms
step 1
what are usable forms of nitrogen - Answer ammonium, nitrites, and nitrates
step 1
the fixation of nitrogen by bacteria that resides in soil and legume root nodules into usable
forms of nitrogen are done through - Answer the Nitrogen Cycle
, step 1
what is the endpoint of nitrogen fixation - Answer ammonium
step 1
what is reduced to ammonium - Answer nitrates and nitrites
step 1
it is ____ that is used in step 2 of nitrogen assimilation - Answer ammonium
step 1
what is the overall reaction of nitrogen fixation - Answer N2 + 10 H+ + 16 ATP + 8 e- → 2 NH4+
+ H2 + 16 ADP + 16 Pi
what is a requirement for the fixation of nitrogen - Answer energy requirement
it does not come cheap but it is an absolute necessity so that plants and subsequently animals
can obtain the nitrogen in usable form
where does step 2 of nitrogen assimilation into biomolecules occur - Answer plants and
microorganisms
step 2 of nitrogen assimilation involves the incorporation of - Answer ammonium into
glutamate and glutamine
step 2
with the incorporation of ammonium into glutamate and glutamine, there are ____ enzyme-
catalyzed reactions involved - Answer 3
step 2
the first reaction is catalyzed by what - Answer glutamate dehydrogenase
this was a potentially reversible reaction and that either NADH or NADPH could be used
step 2
The glutamate can then be used in a subsequent reaction to - Answer assimilate even more
nitrogen; this is catalyzed by glutamine synthetase