UAMS HEME LAB PRACTICAL 3
SCRIPT TESTED QUESTIONS
◉ Reticulocyte: Answer: RNA; associated with decreased RBC
survival/hemorrhage, hyperplastic marrow
◉ Pappenheimer body: Answer: Iron; seen in sideroblastic anemia,
thalassemia
◉ • Heinz Body: Answer: Body: Precipitated Hemoglobin; seen in
G6PD deficiency Can only be seen with supravital stain
◉ Ringed Sideroblast: Answer: Iron
◉ Basophilic stippling: Answer: RNA aggregates; seen in thalassemia
and lead poisoning
◉ NRBC: Answer: DNA
◉ Agglutination will affect Answer: MCV
,◉ Increased WWBC will affect Answer: RBC count
◉ Lipemia will affect Hemoglobin, NOT Answer: HCT
◉ Megaloblastic Anemia Answer: Defective DNA synthesis from B12
or Folate poor diet
◉ Megaloblastic Anemia Bone marrow will show Answer:
asynchrony, karryohexis, giant metamyelocytes and bands
Hypersegmented Neutrophils
◉ Megaloblastic Anemia Lab results Answer: Pancytopenia Treat
with replacement vitamin therapy
• Decrease Retic
• Increase Direct and indirect bilirubin and LD
◉ Inherited defect in spectrin Answer: Hereditary Spherocytosis
◉ Hereditary Spherocytosis Answer: Decreased cell surface
Peripheral smear: Spherocytes (MCHC >36%) Polychromasia • 1
Osmotic fragility with hemolysis beginning at a NaCl concentration
◉ Auto Immune Hemolytic Anemia Answer: Spherocytes
Polychromasia +DAT
,◉ Sex-linked, decrease in enzyme Answer: G-6-PD
◉ G-6-PD Oxidant drugs cause Answer: hemoglobin to denature and
precipitate
◉ G-6-PD Most often occurs Answer: hemoglobin in black males
◉ • Heinz bodies seen with Methylene Blue Answer: G-6-PD
◉ Pyruvate Kinase Answer: causing potassium loss as well as
sodium Reduced ATP production results in alterations of RBC
membrane, failure of cation pumps loss, which leads to cell
dehydration
• Peripheral Smear: • Echinocytes
◉ Beta Chain substitution (B6Glu->Val) Answer: Sickle Cell
◉ Sickle Cell Answer: Sickle Solubility + • Confirm with Hb
electrophoresis • Sickle Cells Target Cells
◉ Beta Chain substitute Answer: Hgb C • Hgb E
, ◉ • C crystals • Target cells Answer: Hgb C
◉ Differentiate from C on cellulose and A on citrate Answer: • Hgb E
◉ • Beta Chain substitute Mild Hemolytic Anemia Answer: • Hgb E
◉ RETIC Increased Answer: after treatment for IDA, folate, B12
◉ RETIC VALUES Increased in Answer: spherocytosis
◉ RETIC VALUES aplastic anemia Answer: Normal or decreased
◉ RETIC VALUES Increased after treatment Answer: for IDA, folate,
B12
◉ RETIC VALUES Decreased in Answer: untreated Iron, B12, Folate
deficiency
◉ Calculate the indices of a Hgb of 8.0 g/dL. Answer: Cannot
determine.
◉ When is basophilic stippling observed? Answer: Thalassemia, lead
poisoning, megaloblastic anemia, sickle cell
SCRIPT TESTED QUESTIONS
◉ Reticulocyte: Answer: RNA; associated with decreased RBC
survival/hemorrhage, hyperplastic marrow
◉ Pappenheimer body: Answer: Iron; seen in sideroblastic anemia,
thalassemia
◉ • Heinz Body: Answer: Body: Precipitated Hemoglobin; seen in
G6PD deficiency Can only be seen with supravital stain
◉ Ringed Sideroblast: Answer: Iron
◉ Basophilic stippling: Answer: RNA aggregates; seen in thalassemia
and lead poisoning
◉ NRBC: Answer: DNA
◉ Agglutination will affect Answer: MCV
,◉ Increased WWBC will affect Answer: RBC count
◉ Lipemia will affect Hemoglobin, NOT Answer: HCT
◉ Megaloblastic Anemia Answer: Defective DNA synthesis from B12
or Folate poor diet
◉ Megaloblastic Anemia Bone marrow will show Answer:
asynchrony, karryohexis, giant metamyelocytes and bands
Hypersegmented Neutrophils
◉ Megaloblastic Anemia Lab results Answer: Pancytopenia Treat
with replacement vitamin therapy
• Decrease Retic
• Increase Direct and indirect bilirubin and LD
◉ Inherited defect in spectrin Answer: Hereditary Spherocytosis
◉ Hereditary Spherocytosis Answer: Decreased cell surface
Peripheral smear: Spherocytes (MCHC >36%) Polychromasia • 1
Osmotic fragility with hemolysis beginning at a NaCl concentration
◉ Auto Immune Hemolytic Anemia Answer: Spherocytes
Polychromasia +DAT
,◉ Sex-linked, decrease in enzyme Answer: G-6-PD
◉ G-6-PD Oxidant drugs cause Answer: hemoglobin to denature and
precipitate
◉ G-6-PD Most often occurs Answer: hemoglobin in black males
◉ • Heinz bodies seen with Methylene Blue Answer: G-6-PD
◉ Pyruvate Kinase Answer: causing potassium loss as well as
sodium Reduced ATP production results in alterations of RBC
membrane, failure of cation pumps loss, which leads to cell
dehydration
• Peripheral Smear: • Echinocytes
◉ Beta Chain substitution (B6Glu->Val) Answer: Sickle Cell
◉ Sickle Cell Answer: Sickle Solubility + • Confirm with Hb
electrophoresis • Sickle Cells Target Cells
◉ Beta Chain substitute Answer: Hgb C • Hgb E
, ◉ • C crystals • Target cells Answer: Hgb C
◉ Differentiate from C on cellulose and A on citrate Answer: • Hgb E
◉ • Beta Chain substitute Mild Hemolytic Anemia Answer: • Hgb E
◉ RETIC Increased Answer: after treatment for IDA, folate, B12
◉ RETIC VALUES Increased in Answer: spherocytosis
◉ RETIC VALUES aplastic anemia Answer: Normal or decreased
◉ RETIC VALUES Increased after treatment Answer: for IDA, folate,
B12
◉ RETIC VALUES Decreased in Answer: untreated Iron, B12, Folate
deficiency
◉ Calculate the indices of a Hgb of 8.0 g/dL. Answer: Cannot
determine.
◉ When is basophilic stippling observed? Answer: Thalassemia, lead
poisoning, megaloblastic anemia, sickle cell
