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Institution: Portage Learning
Course Code: BIOL 271
Course Title: Microbiology Lab
Exam: Lab 4 Exams
Topic: Microbial Cultivation And Types Of Growth Media
Edition: Updated Version
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Instructions:
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➢ True Or False:
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,Microbial Cultivation And Types Of Growth Media
Question 1.
In Which Phase Of Microbial Growth Does The Number Of Dead Microbial Cells
Exceed The Number Of Live Cells?
A. Lag
B. Log
C. Stationary
D. Death
ANS. D.
Explanation: During The Death Phase, The Number Of Dead Microbial Cells Exceeds
The Number Of Live Cells.
Question 2.
Colonies That Arise From Different Types Of Organisms Often Exhibit Different
Morphologies, Which Can Be Used To Help Identify The Microorganism Type. (True Or
False)
A. True
B. False
ANS. A.
Explanation: Different Microorganisms Can Produce Colonies With Distinct
Morphologies That Help With Identification.
Question 3.
There Is Only One Way To Properly Plate A Microbial Sample. (True Or False)
A. True
B. False.
ANS. B.
Explanation: There Are Multiple Methods For Properly Plating Microbial Samples.
,Question 4.
Agar Is A Type Of:
A. Solid Media
B. Liquid Media
C. Selective Media
D. Differential Media
ANS. A.
Explanation: Agar Is Commonly Used As A Solidifying Agent To Create Solid Media
For Microbial Cultivation.
Question 5.
Colonies That Arise From Different Types Of Microorganisms Often Exhibit
Unique____ That Can Aid In Identifying The Microorganisms.
A. Morphologies
B. Nutrients
C. Enzymes
D. Pigments
ANS. A.
Explanation: Colony Morphology Refers To Observable Characteristics Of Colonies
That Can Help Identify Microorganisms.
Question 6.
Who Discovered Penicillin?
A. Louis Pasteur
B. Alexander Fleming
C. Robert Koch
D. Joseph Lister
ANS. B.
Explanation: Alexander Fleming Discovered Penicillin In 1928 After Observing That A
Mold Inhibited Bacterial Growth.
,Question 7.
Select All The Materials Needed To Complete The Spread/ Patch Method For Screening
Antibiotic Production:
A. ESKAPE Relative Tester Bacteria In Liquid Media
B. Master Plate With Bacteria Isolates
C. Sterile Toothpick Or Loop For Transfer
D. Media Plates
E. All Of The Above
ANS. E.
Explanation: The Spread/Patch Method Requires All Of The Listed Materials To Screen
For Antibiotic Production.
Question 8.
The Spread-Patch Protocol Test For Antibiotic Production Assumes Microbe-Microbe
Interactions Will Trigger The Expression Of The Production Of Antibiotics. (True Or
False)
A. True
B. False
ANS. A.
Explanation: The Protocol Is Based On The Premise That Interactions Between
Microorganisms Can Stimulate Antibiotic Production.
Question 9.
Growth Media Must Provide A Nitrogen Source For Energy Production. (True Or False)
A. True
B. False
ANS. B.
Explanation: Nitrogen Is Primarily Required For Biosynthesis Of Cellular Components
Such As Proteins And Nucleic Acids, Rather Than Directly Serving As The Primary
Energy Source.
,Question 10.
In Which Phase Of Microbial Growth Are Microorganisms Intensely Metabolic With
Little Change In The Number Of Cells?
A. Lag
B. Log
C. Stationary
D. Death
ANS. A.
Explanation: During The Lag Phase, Microorganisms Are Metabolically Active And
Adapt To The Growth Environment, But There Is Little Increase In Cell Number.
Question 11.
Microorganisms In Growth Media Demonstrate Consistent Exponential Growth. (True Or
False)
A. True
B. False
ANS. B.
Explanation: Microbial Growth Typically Progresses Through Distinct Phases Rather
Than Remaining In Consistent Exponential Growth.
Question 12.
Identify The Type Of Media Shown In The Image Below. These Media Are Designed To
Grow A Broad Spectrum Of Microbes That Do Not Have Special Growth Requirements.
, A. General Purpose
B. Enriched
C. Specimen Transport
D. Anaerobic Growth
E. Selective
F. Differential
G. Enumeration
H. Assay
ANS. A.
Explanation: General-Purpose Media Are Formulated To Support The Growth Of A
Wide Variety Of Microorganisms That Do Not Require Specialized Nutrients Or Growth
Conditions.
Question 13
Identify The Type Of Media Shown In The Image Below. These Media Contain Complex
Organic Substances, Such As Growth Factors, That Are Required For Microbes To
Grow.
A. General Purpose
B. Enriched
C. Specimen Transport
D. Anaerobic Growth
E. Selective
F. Differential